Screening of laccase, manganese peroxidase, and versatile peroxidase activities of the genus Pleurotus in media with some raw plant materials as carbon sources
Само за регистроване кориснике
2004
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Species of the genus Pleurotus are among the most efficient natural species in lignin degradation belonging to the subclass of ligninolytic organisms that produce laccase (Lac), Mn-dependent peroxidase (MnP), versatile peroxidase (VP), and the H2O2-generating enzyme aryl-alcohol oxidase, but not lignin peroxidases. Production of Lac and oxidation of 2,6-dimethoxyphenol (DMP) in the presence and absence of Mn2+ were detected both in submerged fermentation (SF) of dry ground mandarine peels and in solid-state fermentation (SSF) of grapevine sawdust in all investigated Pleurotus species and strains. Evidence of cultivation methods having a distinct influence on the level of enzyme activities has been demonstrated. Most of the species and strains had higher Lac activity under SSF conditions than under SF conditions. DMP oxidation in the presence and absence of Mn2+ was detected in all investigated species and strains, but was lower under SF conditions than under SSF conditions for most of ...them. However, relative activities of DMP oxidation in the absence of Mn2+, as percentages of activity against DMP in the presence of Mn2+, were higher under conditions of SF than in SSF cultures in most of the investigated species and strains. The obtained results showed that strains of different origins have different efficiently ligninolytic systems and that conditions of SSF are more favorable for ligninolytic activity than those in SF owing to their similarity to natural conditions on wood substrates.
Кључне речи:
Pleurotus / laccase / manganese peroxidase / versatile peroxidase / submerged fermentation / solid-state fermentationИзвор:
Applied Biochemistry and Biotechnology, 2004, 117, 3, 155-164Издавач:
- Humana Press Inc, Totowa
DOI: 10.1385/ABAB:117:3:155
ISSN: 0273-2289
PubMed: 15304767
WoS: 000223321000003
Scopus: 2-s2.0-4344686846
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Stajić, Mirjana AU - Persky, L AU - Cohen, E AU - Hadar, Y AU - Brčeski, Ilija AU - Wasser, SP AU - Nevo, E PY - 2004 UR - https://cherry.chem.bg.ac.rs/handle/123456789/653 AB - Species of the genus Pleurotus are among the most efficient natural species in lignin degradation belonging to the subclass of ligninolytic organisms that produce laccase (Lac), Mn-dependent peroxidase (MnP), versatile peroxidase (VP), and the H2O2-generating enzyme aryl-alcohol oxidase, but not lignin peroxidases. Production of Lac and oxidation of 2,6-dimethoxyphenol (DMP) in the presence and absence of Mn2+ were detected both in submerged fermentation (SF) of dry ground mandarine peels and in solid-state fermentation (SSF) of grapevine sawdust in all investigated Pleurotus species and strains. Evidence of cultivation methods having a distinct influence on the level of enzyme activities has been demonstrated. Most of the species and strains had higher Lac activity under SSF conditions than under SF conditions. DMP oxidation in the presence and absence of Mn2+ was detected in all investigated species and strains, but was lower under SF conditions than under SSF conditions for most of them. However, relative activities of DMP oxidation in the absence of Mn2+, as percentages of activity against DMP in the presence of Mn2+, were higher under conditions of SF than in SSF cultures in most of the investigated species and strains. The obtained results showed that strains of different origins have different efficiently ligninolytic systems and that conditions of SSF are more favorable for ligninolytic activity than those in SF owing to their similarity to natural conditions on wood substrates. PB - Humana Press Inc, Totowa T2 - Applied Biochemistry and Biotechnology T1 - Screening of laccase, manganese peroxidase, and versatile peroxidase activities of the genus Pleurotus in media with some raw plant materials as carbon sources VL - 117 IS - 3 SP - 155 EP - 164 DO - 10.1385/ABAB:117:3:155 ER -
@article{ author = "Stajić, Mirjana and Persky, L and Cohen, E and Hadar, Y and Brčeski, Ilija and Wasser, SP and Nevo, E", year = "2004", abstract = "Species of the genus Pleurotus are among the most efficient natural species in lignin degradation belonging to the subclass of ligninolytic organisms that produce laccase (Lac), Mn-dependent peroxidase (MnP), versatile peroxidase (VP), and the H2O2-generating enzyme aryl-alcohol oxidase, but not lignin peroxidases. Production of Lac and oxidation of 2,6-dimethoxyphenol (DMP) in the presence and absence of Mn2+ were detected both in submerged fermentation (SF) of dry ground mandarine peels and in solid-state fermentation (SSF) of grapevine sawdust in all investigated Pleurotus species and strains. Evidence of cultivation methods having a distinct influence on the level of enzyme activities has been demonstrated. Most of the species and strains had higher Lac activity under SSF conditions than under SF conditions. DMP oxidation in the presence and absence of Mn2+ was detected in all investigated species and strains, but was lower under SF conditions than under SSF conditions for most of them. However, relative activities of DMP oxidation in the absence of Mn2+, as percentages of activity against DMP in the presence of Mn2+, were higher under conditions of SF than in SSF cultures in most of the investigated species and strains. The obtained results showed that strains of different origins have different efficiently ligninolytic systems and that conditions of SSF are more favorable for ligninolytic activity than those in SF owing to their similarity to natural conditions on wood substrates.", publisher = "Humana Press Inc, Totowa", journal = "Applied Biochemistry and Biotechnology", title = "Screening of laccase, manganese peroxidase, and versatile peroxidase activities of the genus Pleurotus in media with some raw plant materials as carbon sources", volume = "117", number = "3", pages = "155-164", doi = "10.1385/ABAB:117:3:155" }
Stajić, M., Persky, L., Cohen, E., Hadar, Y., Brčeski, I., Wasser, S.,& Nevo, E.. (2004). Screening of laccase, manganese peroxidase, and versatile peroxidase activities of the genus Pleurotus in media with some raw plant materials as carbon sources. in Applied Biochemistry and Biotechnology Humana Press Inc, Totowa., 117(3), 155-164. https://doi.org/10.1385/ABAB:117:3:155
Stajić M, Persky L, Cohen E, Hadar Y, Brčeski I, Wasser S, Nevo E. Screening of laccase, manganese peroxidase, and versatile peroxidase activities of the genus Pleurotus in media with some raw plant materials as carbon sources. in Applied Biochemistry and Biotechnology. 2004;117(3):155-164. doi:10.1385/ABAB:117:3:155 .
Stajić, Mirjana, Persky, L, Cohen, E, Hadar, Y, Brčeski, Ilija, Wasser, SP, Nevo, E, "Screening of laccase, manganese peroxidase, and versatile peroxidase activities of the genus Pleurotus in media with some raw plant materials as carbon sources" in Applied Biochemistry and Biotechnology, 117, no. 3 (2004):155-164, https://doi.org/10.1385/ABAB:117:3:155 . .