The possibility of determining N-acetyl-beta-D-glucosaminidase isoenzymes under alkaline conditions
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Objectives: To have a reliable diagnostic test, the influence of urine pH on the determination of the total activity of N-acetyl-beta-D-glucosarninidase (NAG) and NAG isoenzyme activities was studied. Design and methods: After ultrafiltration and dialysis of the acidic and alkaline urines, the B, A, and A(2) forms of NAG were separated by ion-exchange chromatography on DEAE cellulose. Results: A significant decrease in the total activity of NAG in alkaline urines (pH around 8 or higher) was found, which makes this determination unreliable. Analysis of the isoenzymic profiles obtained for weakly acidic and alkaline urines (in the pH range from 5.5. to 10.0) showed that the percent fractions of the individual isoenzyme activities in the total NAG activity and their ratios changed only at pH values above 9.5. Conclusions: The determination of the denoted isoenzymes of urinary NAG after ultrafiltration, dialysis, and chromatographic separation on DEAE cellulose is reliable in a wide range ...of alkaline pH values Of urine. (c) 2005 The Canadian Society of Clinical Chemists. All rights reserved.
Keywords:N-acetyl-beta-D-glucosaminidase (NAG) / isoenzymes A, B, and A(2) / isoenzyme stabilities / alkaline conditions / determination
Source:Clinical Biochemistry, 2005, 38, 4, 384-389
- Pergamon-Elsevier Science Ltd, Oxford