Show simple item record

dc.creatorMandić, Ljuba M.
dc.creatorAćimović, Jelena M.
dc.creatorJovanović, Vesna B.
dc.date.accessioned2018-11-22T00:08:08Z
dc.date.available2018-11-22T00:08:08Z
dc.date.issued2005
dc.identifier.issn0009-9120
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/692
dc.description.abstractObjectives: To have a reliable diagnostic test, the influence of urine pH on the determination of the total activity of N-acetyl-beta-D-glucosarninidase (NAG) and NAG isoenzyme activities was studied. Design and methods: After ultrafiltration and dialysis of the acidic and alkaline urines, the B, A, and A(2) forms of NAG were separated by ion-exchange chromatography on DEAE cellulose. Results: A significant decrease in the total activity of NAG in alkaline urines (pH around 8 or higher) was found, which makes this determination unreliable. Analysis of the isoenzymic profiles obtained for weakly acidic and alkaline urines (in the pH range from 5.5. to 10.0) showed that the percent fractions of the individual isoenzyme activities in the total NAG activity and their ratios changed only at pH values above 9.5. Conclusions: The determination of the denoted isoenzymes of urinary NAG after ultrafiltration, dialysis, and chromatographic separation on DEAE cellulose is reliable in a wide range of alkaline pH values Of urine. (c) 2005 The Canadian Society of Clinical Chemists. All rights reserved.en
dc.publisherPergamon-Elsevier Science Ltd, Oxford
dc.rightsrestrictedAccess
dc.sourceClinical Biochemistry
dc.subjectN-acetyl-beta-D-glucosaminidase (NAG)en
dc.subjectisoenzymes A, B, and A(2)en
dc.subjectisoenzyme stabilitiesen
dc.subjectalkaline conditionsen
dc.subjectdeterminationen
dc.titleThe possibility of determining N-acetyl-beta-D-glucosaminidase isoenzymes under alkaline conditionsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЈовановић, Весна; Мандић, Љуба М.; Aћимовић, Јелена;
dc.citation.volume38
dc.citation.issue4
dc.citation.spage384
dc.citation.epage389
dc.identifier.wos000228116600011
dc.identifier.doi10.1016/j.clinbiochem.2005.01.002
dc.citation.other38(4): 384-389
dc.citation.rankM21
dc.identifier.pmid15766740
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-14844328058
dc.identifier.rcubKon_1645


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record