Приказ основних података о документу
Detection and quantification of leucyl arninopeptidase after native electrophoresis using leucine-p-nitroanilide
dc.creator | Božić, Nataša | |
dc.creator | Vujčić, Zoran | |
dc.date.accessioned | 2018-11-22T00:08:29Z | |
dc.date.available | 2018-11-22T00:08:29Z | |
dc.date.issued | 2005 | |
dc.identifier.issn | 0173-0835 | |
dc.identifier.uri | https://cherry.chem.bg.ac.rs/handle/123456789/714 | |
dc.description.abstract | A general method for detecting leucyl aminopeptidase activity after native polyacrylamide gel electrophoresis (PAGE) in situ is described. The method is based on diazotization of p-nitroaniline, liberated in the polyacrylamide gel by leucyl aminopeptidase action on leucine-p-nitroanilide (LpNA) and subsequent coupling with a chromogen, 1-naphthylamine, until a pink azo dye product at the position of enzyme activity is obtained. A possible use of this technique for leucyl aminopeptidase detection and quantification is indicated. This method was found to be reproducible with the coefficient of variation below 15% for a 32-fold range, while the colored area of enzyme activity was in linear dependence to enzyme activity. Applications of this method with some other aminoacyl-p-nitroanilides and for detection of kidney bean leucyl aminopeptidase isoforms are demonstrated. | en |
dc.publisher | Wiley-V C H Verlag Gmbh, Weinheim | |
dc.rights | restrictedAccess | |
dc.source | Electrophoresis | |
dc.subject | isoenzymes | en |
dc.subject | leucyl aminopeptidase | en |
dc.subject | Phaseolus vulgaris | en |
dc.subject | p-nitroanilide | en |
dc.subject | zymogram | en |
dc.title | Detection and quantification of leucyl arninopeptidase after native electrophoresis using leucine-p-nitroanilide | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Бозиц, НA; Вујчић, Зоран; | |
dc.citation.volume | 26 | |
dc.citation.issue | 12 | |
dc.citation.spage | 2476 | |
dc.citation.epage | 2480 | |
dc.identifier.wos | 000230239900022 | |
dc.identifier.doi | 10.1002/elps.200500047 | |
dc.citation.other | 26(12): 2476-2480 | |
dc.citation.rank | aM21 | |
dc.identifier.pmid | 15920779 | |
dc.type.version | publishedVersion | en |
dc.identifier.scopus | 2-s2.0-21644465014 |