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dc.creatorStankov-Jovanović, Vesna P.
dc.creatorNikolić-Mandić, Snezana D.
dc.creatorMandić, Ljuba M.
dc.creatorMitic, Violeta D.
dc.date.accessioned2018-11-22T00:09:56Z
dc.date.available2018-11-22T00:09:56Z
dc.date.issued2006
dc.identifier.issn1618-2642
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/794
dc.description.abstractPancuronium bromide (PCBr) inhibition effect on enzyme cholinesterase from pooled human serum (Che, EC 3.1.1.8 acylcholine acylhydrolase) was used for development of a spectrophotometric kinetic method for PCBr determination in human serum and urine. Optimal conditions for the basic and inhibitor reactions were established: pH=7.7 and substrate concentration c(benzoylcholine chloride)=1.33 mmol/L. Kinetic parameters were also determined: Michaelis-Menten's constant K-M=0.40 mmol/L, maximal reaction rate V-max=52.2 mu mol/L min, inhibition constant K-i=0,56 mu mol/L and IC50=1.31 mu mol/L. Linear dependence between the reaction rate and inhibitor concentration exists in PCBr concentration range 8.20-68.25 nmol/L, which corresponds to the real sample concentrations from 0.328 to 2.730 mu mol/L. The method detection and quantification limits were 2.01 nmol/L and 6.67 nmol/L, respectively. Precision of the method was tested for three pancuronium concentrations (10.70, 29.35 and 51.25 nmol/L). Relative standard deviation (RSD) was in the range 0.15-7.45%. Accuracy was examined by standard addition method. Influence of the substances usually present in serum and urine on the reaction rate was tested. The developed method was applied for PCBr content determination in serum model samples, urine model samples and in urine taken during surgery. The method has good sensitivity, accuracy, precision and it is suitable for clinical practice.en
dc.publisherSpringer Heidelberg, Heidelberg
dc.rightsrestrictedAccess
dc.sourceAnalytical and Bioanalytical Chemistry
dc.subjectpancuronium bromideen
dc.subjectcholinesterase inhibitionen
dc.subjectbenzoylcholineen
dc.subjectspectrophotometric kinetic determinationen
dc.titleCholinesterase inhibition based determination of pancuronium bromide in biological samplesen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractСтанков-Јовановиц, Весна П.; Николиц-Мандиц, Снезана Д.; Мандић, Љуба М.; Митиц, Виолета Д.;
dc.citation.volume385
dc.citation.issue8
dc.citation.spage1462
dc.citation.epage1469
dc.identifier.wos000239644200017
dc.identifier.doi10.1007/s00216-006-0556-5
dc.citation.other385(8): 1462-1469
dc.citation.rankM21
dc.identifier.pmid16804673
dc.identifier.rcubKon_1747


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Приказ основних података о документу