A modification of the kinetic determination of pancuronium bromide based on its inhibitory effect on cholinesterase
Само за регистроване кориснике
2007
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A modification of the existing spectrophotometric kinetic method for the determination of pancuronium bromide (PCBr), based on pooled human serum cholinesterase (ChE, EC 3.1.1.8 acylcholine acylhydrolase) inhibition, was developed. Butyrylthiocholine iodide (concentration 1.667 mmol/L) was used as substrate and determination was performed at pH 7.6. Essential basic kinetic parameters were also determined: Michaelis-Menten's constant K-M=0.33 mmol/L, maximal reaction rate V-max=42.29 mu mol/ L min, inhibition constant K-I = 0.34 mu mol/L, and IC50=0.235 mu mol/L. Linear dependence between the reaction rate and the inhibitor concentration exists in PCBr concentration range 8.29-265.28 nmol/L, which corresponds to the real sample concentrations from 0.166 to 5.306 mu mol/L. The method detection limit was established to be 1.86 nmol/L and the quantification limit was 6.18 nmol/L. Precision of the method was tested for three pancuronium concentrations (16.58, 99.48, and 198.96 nmol/L). The ...relative standard deviation (RSD) was in the range 0.78-5.13%. Accuracy was examined by the standard addition method. The influence of substances usually present in serum and urine on the reaction rate was determined. The method developed was applied for PCBr determination in spiked serum and urine samples and in the urine taken during surgery. The method was proven to have good sensitivity, accuracy, and precision and can be considered suitable for clinical practice.
Кључне речи:
cholinesterase inhibition / pancuronium bromide / buthyrylthiocholine / kinetic determinationИзвор:
Journal of Clinical Laboratory Analysis, 2007, 21, 2, 124-131Издавач:
- Wiley-Liss, Hoboken
DOI: 10.1002/jcla.20162
ISSN: 0887-8013
PubMed: 17385680
WoS: 000245369000013
Scopus: 2-s2.0-34047230734
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Stankov-Jovanović, Vesna P. AU - Nikolić-Mandić, Snežana D. AU - Mandić, Ljuba M. AU - Mitić, Violeta D. PY - 2007 UR - https://cherry.chem.bg.ac.rs/handle/123456789/825 AB - A modification of the existing spectrophotometric kinetic method for the determination of pancuronium bromide (PCBr), based on pooled human serum cholinesterase (ChE, EC 3.1.1.8 acylcholine acylhydrolase) inhibition, was developed. Butyrylthiocholine iodide (concentration 1.667 mmol/L) was used as substrate and determination was performed at pH 7.6. Essential basic kinetic parameters were also determined: Michaelis-Menten's constant K-M=0.33 mmol/L, maximal reaction rate V-max=42.29 mu mol/ L min, inhibition constant K-I = 0.34 mu mol/L, and IC50=0.235 mu mol/L. Linear dependence between the reaction rate and the inhibitor concentration exists in PCBr concentration range 8.29-265.28 nmol/L, which corresponds to the real sample concentrations from 0.166 to 5.306 mu mol/L. The method detection limit was established to be 1.86 nmol/L and the quantification limit was 6.18 nmol/L. Precision of the method was tested for three pancuronium concentrations (16.58, 99.48, and 198.96 nmol/L). The relative standard deviation (RSD) was in the range 0.78-5.13%. Accuracy was examined by the standard addition method. The influence of substances usually present in serum and urine on the reaction rate was determined. The method developed was applied for PCBr determination in spiked serum and urine samples and in the urine taken during surgery. The method was proven to have good sensitivity, accuracy, and precision and can be considered suitable for clinical practice. PB - Wiley-Liss, Hoboken T2 - Journal of Clinical Laboratory Analysis T1 - A modification of the kinetic determination of pancuronium bromide based on its inhibitory effect on cholinesterase VL - 21 IS - 2 SP - 124 EP - 131 DO - 10.1002/jcla.20162 ER -
@article{ author = "Stankov-Jovanović, Vesna P. and Nikolić-Mandić, Snežana D. and Mandić, Ljuba M. and Mitić, Violeta D.", year = "2007", abstract = "A modification of the existing spectrophotometric kinetic method for the determination of pancuronium bromide (PCBr), based on pooled human serum cholinesterase (ChE, EC 3.1.1.8 acylcholine acylhydrolase) inhibition, was developed. Butyrylthiocholine iodide (concentration 1.667 mmol/L) was used as substrate and determination was performed at pH 7.6. Essential basic kinetic parameters were also determined: Michaelis-Menten's constant K-M=0.33 mmol/L, maximal reaction rate V-max=42.29 mu mol/ L min, inhibition constant K-I = 0.34 mu mol/L, and IC50=0.235 mu mol/L. Linear dependence between the reaction rate and the inhibitor concentration exists in PCBr concentration range 8.29-265.28 nmol/L, which corresponds to the real sample concentrations from 0.166 to 5.306 mu mol/L. The method detection limit was established to be 1.86 nmol/L and the quantification limit was 6.18 nmol/L. Precision of the method was tested for three pancuronium concentrations (16.58, 99.48, and 198.96 nmol/L). The relative standard deviation (RSD) was in the range 0.78-5.13%. Accuracy was examined by the standard addition method. The influence of substances usually present in serum and urine on the reaction rate was determined. The method developed was applied for PCBr determination in spiked serum and urine samples and in the urine taken during surgery. The method was proven to have good sensitivity, accuracy, and precision and can be considered suitable for clinical practice.", publisher = "Wiley-Liss, Hoboken", journal = "Journal of Clinical Laboratory Analysis", title = "A modification of the kinetic determination of pancuronium bromide based on its inhibitory effect on cholinesterase", volume = "21", number = "2", pages = "124-131", doi = "10.1002/jcla.20162" }
Stankov-Jovanović, V. P., Nikolić-Mandić, S. D., Mandić, L. M.,& Mitić, V. D.. (2007). A modification of the kinetic determination of pancuronium bromide based on its inhibitory effect on cholinesterase. in Journal of Clinical Laboratory Analysis Wiley-Liss, Hoboken., 21(2), 124-131. https://doi.org/10.1002/jcla.20162
Stankov-Jovanović VP, Nikolić-Mandić SD, Mandić LM, Mitić VD. A modification of the kinetic determination of pancuronium bromide based on its inhibitory effect on cholinesterase. in Journal of Clinical Laboratory Analysis. 2007;21(2):124-131. doi:10.1002/jcla.20162 .
Stankov-Jovanović, Vesna P., Nikolić-Mandić, Snežana D., Mandić, Ljuba M., Mitić, Violeta D., "A modification of the kinetic determination of pancuronium bromide based on its inhibitory effect on cholinesterase" in Journal of Clinical Laboratory Analysis, 21, no. 2 (2007):124-131, https://doi.org/10.1002/jcla.20162 . .