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Da li holesterol vezan za hemoglobin utiče na anti-oksidativni enzimski sistem u humanim eritrocitima?

dc.creatorNikolić, Milan
dc.creatorKokic, Aleksandra Nikolic
dc.creatorStanic, Dragana
dc.creatorBlagojević, Duško P.
dc.creatorVranic, Danijela
dc.creatorJones, David R.
dc.creatorNiketic, Vesna
dc.creatorSpasic, Mihajlo B.
dc.date.accessioned2018-11-22T00:10:56Z
dc.date.available2018-11-22T00:10:56Z
dc.date.issued2007
dc.identifier.issn0352-5139
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/837
dc.description.abstractIn a previous study, it was shown that the lipid fraction, which is occasionally observed in red blood cell hemolysates, represents cholesterol (Ch) associated with phospholipid firmly bound to haemoglobin (termed Hb-Ch). The current study was conducted to investigate whether Hb-Ch could affect the primary anti-oxidant enzyme defence system in human erythrocytes. Sixty healthy volunteers were used for the current study. Group 1 consisted of 28 subjects without or with a low level of Hb-Ch. Group 2 comprised 32 subjects with a considerably higher level of Hb-Ch. The activities of erythrocyte superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase, as well as the content of methaemoglobin (metHb) were measured in both groups. The results indicated that the amount of Hb-Ch neither influenced the activities of the erythrocyte anti-oxidant enzymes nor altered the level of metHb. However, a higher amount of Hb-Ch changed the correlations in the part of the anti-oxidant defence system relating to glutathione, suggesting increased peroxidative pressure from plasma lipids. Group 2 also had significantly increased concentrations of total plasma Ch and triglycerides. Together, these facts are strong indications that the anti-oxidant defence system in human erythrocytes finely retunes its composition according to plasma oxidative demands.en
dc.description.abstractU prethodnom radu pokazano je da lipidna frakcija koja se javlja u hemolizatu zdravih ljudi predstavlja holesterol (asosovan sa fosfolipidima) čvrsto vezan za hemoglobin (Hb-Ch). U ovom radu ispitivan je uticaj Hb-Ch na anti-oksidativni enzimski sistem u humanim eritrocitima. Određena je aktivnost superoksid-dizmutaze, katalaze, glutation-peroksidaze i glutation-reduktaze, kao i sadržaj met-hemoglobina (metHb) u eritrocitima 60 ljudi, podeljenih u dve grupe na osnovu količine Hb-Ch. Rezultati pokazuju da količina prisutnog Hb-Ch ne menja aktivnost merenih enzima, niti nivo metHb. Međutim, u grupi ispitanika sa povećanim sadržajem Hb-Ch zapažene su korelativne promene u delu anti-oksidativnog enzimskog sistema povezanog sa glutationom. U istoj grupi detektovane su i veće koncentracije ukupnog holesterola i triglicerida u plazmi, što zajedno ukazuje na povećani peroksidativni pritisak iz plazme. Ovi rezultati ukazuju da odbrambeni anti-oksidativni enzimski sistem u humanim eritrocitima prilagođava svoju organizaciju prema zahtevima iz svog okruženja. .sr
dc.publisherSerbian Chemical Soc, Belgrade
dc.rightsopenAccess
dc.sourceJournal of the Serbian Chemical Society
dc.subjecterythrocytesen
dc.subjecthaemoglobinen
dc.subjecthaemoglobinen
dc.subjectcholesterolen
dc.subjectcholesterolen
dc.subjectanti-oxidant defence enzymesen
dc.subjectanti-oxidant defence enzymesen
dc.titleDoes cholesterol bound to haemoglobin affect the anti-oxidant enzyme defence system in human erythrocytes?en
dc.titleDa li holesterol vezan za hemoglobin utiče na anti-oksidativni enzimski sistem u humanim eritrocitima?sr
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractНиколић, Милан; Благојевиц, Дуско П.; Спасиц, Михајло Б.; Јонес, Давид Р.; Кокиц, Aлександра Николиц; Никетиц, Весна; Враниц, Данијела; Станиц, Драгана; Да ли холестерол везан за хемоглобин утиче на анти-оксидативни ензимски систем у хуманим еритроцитима?; Да ли холестерол везан за хемоглобин утиче на анти-оксидативни ензимски систем у хуманим еритроцитима?;
dc.citation.volume72
dc.citation.issue4
dc.citation.spage339
dc.citation.epage345
dc.identifier.wos000247047000004
dc.identifier.doi10.2298/JSC0704339N
dc.citation.other72(4): 339-345
dc.citation.rankM23
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-34249823961
dc.identifier.rcubKon_1790


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