The non-proteolytic house dust mite allergen Der p 2 induce NF-kappa B and MAPK dependent activation of bronchial epithelial cells
Само за регистроване кориснике
2009
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
P gt Background House dust mites (HDM) are well-known as a source of indoor aeroallergens and for causing allergic airway diseases. Some proteolytic HDM allergens are known to activate respiratory epithelial cells to produce pro-inflammatory mediators, while there is limited knowledge regarding such activity among non-proteolytic HDM allergens. Objective To investigate whether Der p 2, a major non-proteolytic allergen of Dermatophagoides pteronyssinus, activates respiratory epithelial cells to produce mediators involved in asthma pathogenesis and to elucidate the mechanism of such activation. Methods The human bronchial epithelial cell line BEAS-2B, normal human bronchial epithelial (NHBE) cells and the alveolar epithelial cell line A549 were exposed to recombinant Der p 2. Following exposure, we analysed a panel of soluble mediators and cell adhesion receptors involved in asthma pathogenesis by promoting recruitment, survival and binding of inflammatory cells. The involvement of nucle...ar factor (NF)-kappa B and mitogen-activated protein kinases (MAPKs) was studied using specific inhibitors. Results Der p 2 activated bronchial BEAS-2B and NHBE cells, but not alveolar A549 cells. In BEAS-2B cells Der p 2 induced dose-dependent up-regulation in both mRNA level and protein secretion of granulocyte-macrophage colony-stimulating factor, IL-6, IL-8, monocyte-chemotactic protein-1 and macrophage inflammatory protein-3 alpha. Secretion as well as surface expression of intercellular adhesion molecule (ICAM)-1 was also up-regulated, which was associated with increased adhesion of monocytes to the epithelial cells. The release of cytokines and chemokines was regulated by NF-kappa B and MAPK activation in different ways, while expression of ICAM-1 was solely dependent on NF-kappa B activation. Conclusion These results show that Der p 2 activates respiratory epithelial cells, indicating that this non-proteolytic allergen, in addition to its immunogenic properties, can aggravate respiratory airway disease by adjuvant-like activation of the lung epithelium.
Кључне речи:
Der p 2 / epithelium / house dust mite allergen / inflammation / non-proteolytic / signal transductionИзвор:
Clinical and Experimental Allergy, 2009, 39, 8, 1199-1208Издавач:
- Wiley-Blackwell Publishing, Inc, Malden
Финансирање / пројекти:
- Kempe Foundation
- Swedish Asthma and Allergy Foundation, Vardal Foundation
DOI: 10.1111/j.1365-2222.2009.03284.x
ISSN: 0954-7894
PubMed: 19486032
WoS: 000267880500013
Scopus: 2-s2.0-67650450510
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Osterlund, C. AU - Gronlund, Hans AU - Polović, Natalija AU - Sundstrom, S. AU - Gafvelin, Guro AU - Bucht, A. PY - 2009 UR - https://cherry.chem.bg.ac.rs/handle/123456789/996 AB - P gt Background House dust mites (HDM) are well-known as a source of indoor aeroallergens and for causing allergic airway diseases. Some proteolytic HDM allergens are known to activate respiratory epithelial cells to produce pro-inflammatory mediators, while there is limited knowledge regarding such activity among non-proteolytic HDM allergens. Objective To investigate whether Der p 2, a major non-proteolytic allergen of Dermatophagoides pteronyssinus, activates respiratory epithelial cells to produce mediators involved in asthma pathogenesis and to elucidate the mechanism of such activation. Methods The human bronchial epithelial cell line BEAS-2B, normal human bronchial epithelial (NHBE) cells and the alveolar epithelial cell line A549 were exposed to recombinant Der p 2. Following exposure, we analysed a panel of soluble mediators and cell adhesion receptors involved in asthma pathogenesis by promoting recruitment, survival and binding of inflammatory cells. The involvement of nuclear factor (NF)-kappa B and mitogen-activated protein kinases (MAPKs) was studied using specific inhibitors. Results Der p 2 activated bronchial BEAS-2B and NHBE cells, but not alveolar A549 cells. In BEAS-2B cells Der p 2 induced dose-dependent up-regulation in both mRNA level and protein secretion of granulocyte-macrophage colony-stimulating factor, IL-6, IL-8, monocyte-chemotactic protein-1 and macrophage inflammatory protein-3 alpha. Secretion as well as surface expression of intercellular adhesion molecule (ICAM)-1 was also up-regulated, which was associated with increased adhesion of monocytes to the epithelial cells. The release of cytokines and chemokines was regulated by NF-kappa B and MAPK activation in different ways, while expression of ICAM-1 was solely dependent on NF-kappa B activation. Conclusion These results show that Der p 2 activates respiratory epithelial cells, indicating that this non-proteolytic allergen, in addition to its immunogenic properties, can aggravate respiratory airway disease by adjuvant-like activation of the lung epithelium. PB - Wiley-Blackwell Publishing, Inc, Malden T2 - Clinical and Experimental Allergy T1 - The non-proteolytic house dust mite allergen Der p 2 induce NF-kappa B and MAPK dependent activation of bronchial epithelial cells VL - 39 IS - 8 SP - 1199 EP - 1208 DO - 10.1111/j.1365-2222.2009.03284.x ER -
@article{ author = "Osterlund, C. and Gronlund, Hans and Polović, Natalija and Sundstrom, S. and Gafvelin, Guro and Bucht, A.", year = "2009", abstract = "P gt Background House dust mites (HDM) are well-known as a source of indoor aeroallergens and for causing allergic airway diseases. Some proteolytic HDM allergens are known to activate respiratory epithelial cells to produce pro-inflammatory mediators, while there is limited knowledge regarding such activity among non-proteolytic HDM allergens. Objective To investigate whether Der p 2, a major non-proteolytic allergen of Dermatophagoides pteronyssinus, activates respiratory epithelial cells to produce mediators involved in asthma pathogenesis and to elucidate the mechanism of such activation. Methods The human bronchial epithelial cell line BEAS-2B, normal human bronchial epithelial (NHBE) cells and the alveolar epithelial cell line A549 were exposed to recombinant Der p 2. Following exposure, we analysed a panel of soluble mediators and cell adhesion receptors involved in asthma pathogenesis by promoting recruitment, survival and binding of inflammatory cells. The involvement of nuclear factor (NF)-kappa B and mitogen-activated protein kinases (MAPKs) was studied using specific inhibitors. Results Der p 2 activated bronchial BEAS-2B and NHBE cells, but not alveolar A549 cells. In BEAS-2B cells Der p 2 induced dose-dependent up-regulation in both mRNA level and protein secretion of granulocyte-macrophage colony-stimulating factor, IL-6, IL-8, monocyte-chemotactic protein-1 and macrophage inflammatory protein-3 alpha. Secretion as well as surface expression of intercellular adhesion molecule (ICAM)-1 was also up-regulated, which was associated with increased adhesion of monocytes to the epithelial cells. The release of cytokines and chemokines was regulated by NF-kappa B and MAPK activation in different ways, while expression of ICAM-1 was solely dependent on NF-kappa B activation. Conclusion These results show that Der p 2 activates respiratory epithelial cells, indicating that this non-proteolytic allergen, in addition to its immunogenic properties, can aggravate respiratory airway disease by adjuvant-like activation of the lung epithelium.", publisher = "Wiley-Blackwell Publishing, Inc, Malden", journal = "Clinical and Experimental Allergy", title = "The non-proteolytic house dust mite allergen Der p 2 induce NF-kappa B and MAPK dependent activation of bronchial epithelial cells", volume = "39", number = "8", pages = "1199-1208", doi = "10.1111/j.1365-2222.2009.03284.x" }
Osterlund, C., Gronlund, H., Polović, N., Sundstrom, S., Gafvelin, G.,& Bucht, A.. (2009). The non-proteolytic house dust mite allergen Der p 2 induce NF-kappa B and MAPK dependent activation of bronchial epithelial cells. in Clinical and Experimental Allergy Wiley-Blackwell Publishing, Inc, Malden., 39(8), 1199-1208. https://doi.org/10.1111/j.1365-2222.2009.03284.x
Osterlund C, Gronlund H, Polović N, Sundstrom S, Gafvelin G, Bucht A. The non-proteolytic house dust mite allergen Der p 2 induce NF-kappa B and MAPK dependent activation of bronchial epithelial cells. in Clinical and Experimental Allergy. 2009;39(8):1199-1208. doi:10.1111/j.1365-2222.2009.03284.x .
Osterlund, C., Gronlund, Hans, Polović, Natalija, Sundstrom, S., Gafvelin, Guro, Bucht, A., "The non-proteolytic house dust mite allergen Der p 2 induce NF-kappa B and MAPK dependent activation of bronchial epithelial cells" in Clinical and Experimental Allergy, 39, no. 8 (2009):1199-1208, https://doi.org/10.1111/j.1365-2222.2009.03284.x . .