Characterization of melanin-overproducing transposon mutants of Pseudomonas putida F6
Само за регистроване кориснике
2009
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Two melanin-overproducing Pseudomonas putida F6 mutants were generated using transposon (Tn5) mutagenesis. Mutants were disrupted in a transcriptional regulator (TR) and a homogentisate 1,2-dioxygenase (HDO) gene. Colonies of mutant F6-TR overproduced a black pigment on solid medium. The same mutant (F6-TR) had a 3.7-fold higher tyrosinase activity compared with the wild-type strain when induced with ferulic acid. However in tyrosine uptake assays whole cells of the mutant strain F6-TR consumed eight times less tyrosine compared with the wild-type strain. Mutant F6-HDO produced a diffusible red pigment into the growth medium. Pigment production by mutant F6-HDO is sixfold higher than the wild-type strain. The biomass yield of mutant F6-HDO grown on tyrosine as the sole source of carbon and energy was 1.2-fold lower than the wild-type strain. While the growth of the wild-type strain was completely inhibited by 5 min of exposure to UV light (254 nm) both mutant strains showed survival ra...tes gt 30%. Mutant F6-HDO was able to tolerate higher concentrations of hydrogen peroxide (H(2)O(2)) exhibiting 1.5 times smaller zones of inhibition at 10 mM H(2)O(2) compared with mutant F6-TR and the wild-type strain. The pigments produced by all strains were purified and confirmed to be melanins.
Кључне речи:
melanin / tyrosinase / Pseudomonas putida F6Извор:
FEMS Microbiology Letters / Federation of European Microbiological Societies, 2009, 298, 2, 174-183Издавач:
- Wiley-Blackwell Publishing, Inc, Malden
Напомена:
- Free full text: https://doi.org/10.1111/j.1574-6968.2009.01716.x
DOI: 10.1111/j.1574-6968.2009.01716.x
ISSN: 0378-1097
PubMed: 19624744
WoS: 000269056800006
Scopus: 2-s2.0-68949211949
Колекције
Институција/група
Inovacioni centar / Innovation CentreTY - JOUR AU - Nikodinović-Runić, Jasmina AU - Martin, Leona B. AU - Babu, Ramesh P. AU - Blau, Werner AU - O'Connor, Kevin E. PY - 2009 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1008 AB - Two melanin-overproducing Pseudomonas putida F6 mutants were generated using transposon (Tn5) mutagenesis. Mutants were disrupted in a transcriptional regulator (TR) and a homogentisate 1,2-dioxygenase (HDO) gene. Colonies of mutant F6-TR overproduced a black pigment on solid medium. The same mutant (F6-TR) had a 3.7-fold higher tyrosinase activity compared with the wild-type strain when induced with ferulic acid. However in tyrosine uptake assays whole cells of the mutant strain F6-TR consumed eight times less tyrosine compared with the wild-type strain. Mutant F6-HDO produced a diffusible red pigment into the growth medium. Pigment production by mutant F6-HDO is sixfold higher than the wild-type strain. The biomass yield of mutant F6-HDO grown on tyrosine as the sole source of carbon and energy was 1.2-fold lower than the wild-type strain. While the growth of the wild-type strain was completely inhibited by 5 min of exposure to UV light (254 nm) both mutant strains showed survival rates gt 30%. Mutant F6-HDO was able to tolerate higher concentrations of hydrogen peroxide (H(2)O(2)) exhibiting 1.5 times smaller zones of inhibition at 10 mM H(2)O(2) compared with mutant F6-TR and the wild-type strain. The pigments produced by all strains were purified and confirmed to be melanins. PB - Wiley-Blackwell Publishing, Inc, Malden T2 - FEMS Microbiology Letters / Federation of European Microbiological Societies T1 - Characterization of melanin-overproducing transposon mutants of Pseudomonas putida F6 VL - 298 IS - 2 SP - 174 EP - 183 DO - 10.1111/j.1574-6968.2009.01716.x ER -
@article{ author = "Nikodinović-Runić, Jasmina and Martin, Leona B. and Babu, Ramesh P. and Blau, Werner and O'Connor, Kevin E.", year = "2009", abstract = "Two melanin-overproducing Pseudomonas putida F6 mutants were generated using transposon (Tn5) mutagenesis. Mutants were disrupted in a transcriptional regulator (TR) and a homogentisate 1,2-dioxygenase (HDO) gene. Colonies of mutant F6-TR overproduced a black pigment on solid medium. The same mutant (F6-TR) had a 3.7-fold higher tyrosinase activity compared with the wild-type strain when induced with ferulic acid. However in tyrosine uptake assays whole cells of the mutant strain F6-TR consumed eight times less tyrosine compared with the wild-type strain. Mutant F6-HDO produced a diffusible red pigment into the growth medium. Pigment production by mutant F6-HDO is sixfold higher than the wild-type strain. The biomass yield of mutant F6-HDO grown on tyrosine as the sole source of carbon and energy was 1.2-fold lower than the wild-type strain. While the growth of the wild-type strain was completely inhibited by 5 min of exposure to UV light (254 nm) both mutant strains showed survival rates gt 30%. Mutant F6-HDO was able to tolerate higher concentrations of hydrogen peroxide (H(2)O(2)) exhibiting 1.5 times smaller zones of inhibition at 10 mM H(2)O(2) compared with mutant F6-TR and the wild-type strain. The pigments produced by all strains were purified and confirmed to be melanins.", publisher = "Wiley-Blackwell Publishing, Inc, Malden", journal = "FEMS Microbiology Letters / Federation of European Microbiological Societies", title = "Characterization of melanin-overproducing transposon mutants of Pseudomonas putida F6", volume = "298", number = "2", pages = "174-183", doi = "10.1111/j.1574-6968.2009.01716.x" }
Nikodinović-Runić, J., Martin, L. B., Babu, R. P., Blau, W.,& O'Connor, K. E.. (2009). Characterization of melanin-overproducing transposon mutants of Pseudomonas putida F6. in FEMS Microbiology Letters / Federation of European Microbiological Societies Wiley-Blackwell Publishing, Inc, Malden., 298(2), 174-183. https://doi.org/10.1111/j.1574-6968.2009.01716.x
Nikodinović-Runić J, Martin LB, Babu RP, Blau W, O'Connor KE. Characterization of melanin-overproducing transposon mutants of Pseudomonas putida F6. in FEMS Microbiology Letters / Federation of European Microbiological Societies. 2009;298(2):174-183. doi:10.1111/j.1574-6968.2009.01716.x .
Nikodinović-Runić, Jasmina, Martin, Leona B., Babu, Ramesh P., Blau, Werner, O'Connor, Kevin E., "Characterization of melanin-overproducing transposon mutants of Pseudomonas putida F6" in FEMS Microbiology Letters / Federation of European Microbiological Societies, 298, no. 2 (2009):174-183, https://doi.org/10.1111/j.1574-6968.2009.01716.x . .