Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate
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2012
Authors
Kenny, Shane T.Nikodinović-Runić, Jasmina
Kaminsky, Walter
Woods, Trevor
Babu, Ramesh P.
O'Connor, Kevin E.
Article (Published version)
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Sodium terephthalate (TA) produced from a PET pyrolysis product and waste glycerol (WG) from biodiesel manufacture were supplied to Pseudomonas putida GO16 in a fed-batch bioreactor. Six feeding strategies were employed by altering the sequence of TA and WG feeding. P. putida GO16 reached 8.70 g/l cell dry weight (CDW) and 2.61 g/l PHA in 48 h when grown on TA alone. When TA and WG were supplied in combination, biomass productivity (g/l/h) was increased between 1.3- and 1.7-fold and PHA productivity (g/l/h) was increased 1.8- to 2.2-fold compared to TA supplied alone. The monomer composition of the PHA accumulated from TA or WG was predominantly composed of 3-hydroxydecanoic acid. PHA monomers 3-hydroxytetradeeanoic acid and 3-hydroxytetradecenoic acid were not present in PHA accumulated from TA alone but were present when WG was supplied to the fermentation. When WG was either the sole carbon source or the predominant carbon source supplied to the fermentation the molecular weight of ...PHA accumulated was lower compared to PHA accumulated when TA was supplied as the sole substrate. Despite similarities in data for the properties of the polymers, PHAs produced with WG present in the PHA accumulation phase were tacky while PHA produced where TA was the sole carbon substrate in the polymer accumulation phase exhibited little or no tackiness at room temperature. The co-feeding of WG to fermentations allows for increased utilisation of TA. The order of feeding of WG and TA has an effect on TA utilisation and polymer properties.
Keywords:
Waste / Glycerol / Polyhydroxyalkanoate / Terephthalic acid / Polyethylene terephthalateSource:
Applied Microbiology and Biotechnology, 2012, 95, 3, 623-633Publisher:
- Springer, New York
DOI: 10.1007/s00253-012-4058-4
ISSN: 0175-7598
PubMed: 22581066
WoS: 000306421500007
Scopus: 2-s2.0-84864609421
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Inovacioni centar / Innovation CentreTY - JOUR AU - Kenny, Shane T. AU - Nikodinović-Runić, Jasmina AU - Kaminsky, Walter AU - Woods, Trevor AU - Babu, Ramesh P. AU - O'Connor, Kevin E. PY - 2012 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1319 AB - Sodium terephthalate (TA) produced from a PET pyrolysis product and waste glycerol (WG) from biodiesel manufacture were supplied to Pseudomonas putida GO16 in a fed-batch bioreactor. Six feeding strategies were employed by altering the sequence of TA and WG feeding. P. putida GO16 reached 8.70 g/l cell dry weight (CDW) and 2.61 g/l PHA in 48 h when grown on TA alone. When TA and WG were supplied in combination, biomass productivity (g/l/h) was increased between 1.3- and 1.7-fold and PHA productivity (g/l/h) was increased 1.8- to 2.2-fold compared to TA supplied alone. The monomer composition of the PHA accumulated from TA or WG was predominantly composed of 3-hydroxydecanoic acid. PHA monomers 3-hydroxytetradeeanoic acid and 3-hydroxytetradecenoic acid were not present in PHA accumulated from TA alone but were present when WG was supplied to the fermentation. When WG was either the sole carbon source or the predominant carbon source supplied to the fermentation the molecular weight of PHA accumulated was lower compared to PHA accumulated when TA was supplied as the sole substrate. Despite similarities in data for the properties of the polymers, PHAs produced with WG present in the PHA accumulation phase were tacky while PHA produced where TA was the sole carbon substrate in the polymer accumulation phase exhibited little or no tackiness at room temperature. The co-feeding of WG to fermentations allows for increased utilisation of TA. The order of feeding of WG and TA has an effect on TA utilisation and polymer properties. PB - Springer, New York T2 - Applied Microbiology and Biotechnology T1 - Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate VL - 95 IS - 3 SP - 623 EP - 633 DO - 10.1007/s00253-012-4058-4 ER -
@article{ author = "Kenny, Shane T. and Nikodinović-Runić, Jasmina and Kaminsky, Walter and Woods, Trevor and Babu, Ramesh P. and O'Connor, Kevin E.", year = "2012", abstract = "Sodium terephthalate (TA) produced from a PET pyrolysis product and waste glycerol (WG) from biodiesel manufacture were supplied to Pseudomonas putida GO16 in a fed-batch bioreactor. Six feeding strategies were employed by altering the sequence of TA and WG feeding. P. putida GO16 reached 8.70 g/l cell dry weight (CDW) and 2.61 g/l PHA in 48 h when grown on TA alone. When TA and WG were supplied in combination, biomass productivity (g/l/h) was increased between 1.3- and 1.7-fold and PHA productivity (g/l/h) was increased 1.8- to 2.2-fold compared to TA supplied alone. The monomer composition of the PHA accumulated from TA or WG was predominantly composed of 3-hydroxydecanoic acid. PHA monomers 3-hydroxytetradeeanoic acid and 3-hydroxytetradecenoic acid were not present in PHA accumulated from TA alone but were present when WG was supplied to the fermentation. When WG was either the sole carbon source or the predominant carbon source supplied to the fermentation the molecular weight of PHA accumulated was lower compared to PHA accumulated when TA was supplied as the sole substrate. Despite similarities in data for the properties of the polymers, PHAs produced with WG present in the PHA accumulation phase were tacky while PHA produced where TA was the sole carbon substrate in the polymer accumulation phase exhibited little or no tackiness at room temperature. The co-feeding of WG to fermentations allows for increased utilisation of TA. The order of feeding of WG and TA has an effect on TA utilisation and polymer properties.", publisher = "Springer, New York", journal = "Applied Microbiology and Biotechnology", title = "Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate", volume = "95", number = "3", pages = "623-633", doi = "10.1007/s00253-012-4058-4" }
Kenny, S. T., Nikodinović-Runić, J., Kaminsky, W., Woods, T., Babu, R. P.,& O'Connor, K. E.. (2012). Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate. in Applied Microbiology and Biotechnology Springer, New York., 95(3), 623-633. https://doi.org/10.1007/s00253-012-4058-4
Kenny ST, Nikodinović-Runić J, Kaminsky W, Woods T, Babu RP, O'Connor KE. Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate. in Applied Microbiology and Biotechnology. 2012;95(3):623-633. doi:10.1007/s00253-012-4058-4 .
Kenny, Shane T., Nikodinović-Runić, Jasmina, Kaminsky, Walter, Woods, Trevor, Babu, Ramesh P., O'Connor, Kevin E., "Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate" in Applied Microbiology and Biotechnology, 95, no. 3 (2012):623-633, https://doi.org/10.1007/s00253-012-4058-4 . .