Medium-chain-length polyhydroxyalkanoate production by newly isolated Pseudomonas sp TN301 from a wide range of polyaromatic and monoaromatic hydrocarbons
Само за регистроване кориснике
2012
Аутори
Narancic, T.Kenny, S. T.
Đokić, Lidija
Vasiljević, Branka
O'Connor, K. E.
Nikodinović-Runić, Jasmina
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Aims The aim of this study was to convert numerous polyaromatic and monoaromatic hydrocarbons into biodegradable polymer medium-chain-length polyhydroxyalkanoate (mcl-PHA). Methods and Results Using naphthalene enrichment cultivation method, we have isolated seven bacterial strains from the river sediment exposed to petrochemical industry effluents. In addition to naphthalene, all seven strains could utilize between 12 and 17 different aromatic substrates, including toluene, benzene and biphenyl. Only one isolate that was identified as Pseudomonas sp. TN301 could accumulate mcl-PHA from naphthalene to 23% of cell dry weight. Owing to poor solubility, a method of supplying highly hydrophobic polyaromatic hydrocarbons to a culture medium was developed. The best biomass and mcl-PHA yields were achieved with the addition of synthetic surfactant Tween 80 (0.5 g l-1). We have shown that Pseudomonas sp. TN301 can accumulate mcl-PHA from a wide range of polyaromatic and monoaromatic hydrocarbo...ns, and mixtures thereof, while it could also accumulate polyphosphates and was tolerant to the presence of heavy metal (100 mmol l-1 cadmium and 20 mmol l-1 nickel). Conclusions A new Pseudomonas strain was isolated and identified with the ability to accumulate mcl-PHA from a variety of aromatic hydrocarbons. Significance and Impact of the Study This study is the first report on the ability of a bacterial strain to convert a range of polyaromatic hydrocarbon compounds to the biodegradable polymer (mcl-PHA). Mcl-PHA is gaining importance as a promising biodegradable thermoelastomer, and therefore, isolation of new producing strains is highly significant. Furthermore, this strain has the ability to utilize a range of hydrocarbons, which often occur as mixtures and could potentially be employed in the recently described efforts to convert waste materials to PHA.
Кључне речи:
biopolymers / hazardous waste / pseudomonadsИзвор:
Journal of Applied Microbiology, 2012, 113, 3, 508-520Издавач:
- Wiley-Blackwell, Hoboken
Финансирање / пројекти:
- Изучавање микробиолошког диверзитета и карактеризација корисних срединских микроорганизама (RS-MESTD-Basic Research (BR or ON)-173048)
Напомена:
- Free full text: https://doi.org/10.1111/j.1365-2672.2012.05353.x
DOI: 10.1111/j.1365-2672.2012.05353.x
ISSN: 1364-5072
PubMed: 22642403
WoS: 000307558800004
Scopus: 2-s2.0-84865304278
Колекције
Институција/група
Inovacioni centar / Innovation CentreTY - JOUR AU - Narancic, T. AU - Kenny, S. T. AU - Đokić, Lidija AU - Vasiljević, Branka AU - O'Connor, K. E. AU - Nikodinović-Runić, Jasmina PY - 2012 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1327 AB - Aims The aim of this study was to convert numerous polyaromatic and monoaromatic hydrocarbons into biodegradable polymer medium-chain-length polyhydroxyalkanoate (mcl-PHA). Methods and Results Using naphthalene enrichment cultivation method, we have isolated seven bacterial strains from the river sediment exposed to petrochemical industry effluents. In addition to naphthalene, all seven strains could utilize between 12 and 17 different aromatic substrates, including toluene, benzene and biphenyl. Only one isolate that was identified as Pseudomonas sp. TN301 could accumulate mcl-PHA from naphthalene to 23% of cell dry weight. Owing to poor solubility, a method of supplying highly hydrophobic polyaromatic hydrocarbons to a culture medium was developed. The best biomass and mcl-PHA yields were achieved with the addition of synthetic surfactant Tween 80 (0.5 g l-1). We have shown that Pseudomonas sp. TN301 can accumulate mcl-PHA from a wide range of polyaromatic and monoaromatic hydrocarbons, and mixtures thereof, while it could also accumulate polyphosphates and was tolerant to the presence of heavy metal (100 mmol l-1 cadmium and 20 mmol l-1 nickel). Conclusions A new Pseudomonas strain was isolated and identified with the ability to accumulate mcl-PHA from a variety of aromatic hydrocarbons. Significance and Impact of the Study This study is the first report on the ability of a bacterial strain to convert a range of polyaromatic hydrocarbon compounds to the biodegradable polymer (mcl-PHA). Mcl-PHA is gaining importance as a promising biodegradable thermoelastomer, and therefore, isolation of new producing strains is highly significant. Furthermore, this strain has the ability to utilize a range of hydrocarbons, which often occur as mixtures and could potentially be employed in the recently described efforts to convert waste materials to PHA. PB - Wiley-Blackwell, Hoboken T2 - Journal of Applied Microbiology T1 - Medium-chain-length polyhydroxyalkanoate production by newly isolated Pseudomonas sp TN301 from a wide range of polyaromatic and monoaromatic hydrocarbons VL - 113 IS - 3 SP - 508 EP - 520 DO - 10.1111/j.1365-2672.2012.05353.x ER -
@article{ author = "Narancic, T. and Kenny, S. T. and Đokić, Lidija and Vasiljević, Branka and O'Connor, K. E. and Nikodinović-Runić, Jasmina", year = "2012", abstract = "Aims The aim of this study was to convert numerous polyaromatic and monoaromatic hydrocarbons into biodegradable polymer medium-chain-length polyhydroxyalkanoate (mcl-PHA). Methods and Results Using naphthalene enrichment cultivation method, we have isolated seven bacterial strains from the river sediment exposed to petrochemical industry effluents. In addition to naphthalene, all seven strains could utilize between 12 and 17 different aromatic substrates, including toluene, benzene and biphenyl. Only one isolate that was identified as Pseudomonas sp. TN301 could accumulate mcl-PHA from naphthalene to 23% of cell dry weight. Owing to poor solubility, a method of supplying highly hydrophobic polyaromatic hydrocarbons to a culture medium was developed. The best biomass and mcl-PHA yields were achieved with the addition of synthetic surfactant Tween 80 (0.5 g l-1). We have shown that Pseudomonas sp. TN301 can accumulate mcl-PHA from a wide range of polyaromatic and monoaromatic hydrocarbons, and mixtures thereof, while it could also accumulate polyphosphates and was tolerant to the presence of heavy metal (100 mmol l-1 cadmium and 20 mmol l-1 nickel). Conclusions A new Pseudomonas strain was isolated and identified with the ability to accumulate mcl-PHA from a variety of aromatic hydrocarbons. Significance and Impact of the Study This study is the first report on the ability of a bacterial strain to convert a range of polyaromatic hydrocarbon compounds to the biodegradable polymer (mcl-PHA). Mcl-PHA is gaining importance as a promising biodegradable thermoelastomer, and therefore, isolation of new producing strains is highly significant. Furthermore, this strain has the ability to utilize a range of hydrocarbons, which often occur as mixtures and could potentially be employed in the recently described efforts to convert waste materials to PHA.", publisher = "Wiley-Blackwell, Hoboken", journal = "Journal of Applied Microbiology", title = "Medium-chain-length polyhydroxyalkanoate production by newly isolated Pseudomonas sp TN301 from a wide range of polyaromatic and monoaromatic hydrocarbons", volume = "113", number = "3", pages = "508-520", doi = "10.1111/j.1365-2672.2012.05353.x" }
Narancic, T., Kenny, S. T., Đokić, L., Vasiljević, B., O'Connor, K. E.,& Nikodinović-Runić, J.. (2012). Medium-chain-length polyhydroxyalkanoate production by newly isolated Pseudomonas sp TN301 from a wide range of polyaromatic and monoaromatic hydrocarbons. in Journal of Applied Microbiology Wiley-Blackwell, Hoboken., 113(3), 508-520. https://doi.org/10.1111/j.1365-2672.2012.05353.x
Narancic T, Kenny ST, Đokić L, Vasiljević B, O'Connor KE, Nikodinović-Runić J. Medium-chain-length polyhydroxyalkanoate production by newly isolated Pseudomonas sp TN301 from a wide range of polyaromatic and monoaromatic hydrocarbons. in Journal of Applied Microbiology. 2012;113(3):508-520. doi:10.1111/j.1365-2672.2012.05353.x .
Narancic, T., Kenny, S. T., Đokić, Lidija, Vasiljević, Branka, O'Connor, K. E., Nikodinović-Runić, Jasmina, "Medium-chain-length polyhydroxyalkanoate production by newly isolated Pseudomonas sp TN301 from a wide range of polyaromatic and monoaromatic hydrocarbons" in Journal of Applied Microbiology, 113, no. 3 (2012):508-520, https://doi.org/10.1111/j.1365-2672.2012.05353.x . .