Tlc-Ms Versus Tlc-Lc-Ms Fingerprints of Herbal Extracts. Part Ii. Phenolic Acids and Flavonoids
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Sajewicz, MieczyslawStaszek, Dorota
Natić, Maja
Wojtal, Lukasz
Waksmundzka-Hajnos, Monika
Kowalska, Teresa
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In the previous paper from this series, we proposed mass spectrometric fingerprinting of a complex and volatile botanical sample upon an example of the essential oil derived from Salvia lavandulifolia. In that paper, we compared two variants of fractionation of such a mixture. A simpler one-dimensional variant consisted of the low-temperature thin-layer chromatographic fractionation coupled with mass spectrometric fingerprinting of each separated fraction (1D LT TLC-MS). A more sophisticated variant was the two-dimensional liquid chromatographic system composed of the low-temperature thin-layer chromatography, high-performance liquid chromatography, and mass spectrometric detection (2D LT TLC-LC-MS). In this study, we present an analogous approach to the non-volatile botanical mixtures upon an example of the pharmacologically important phenolic acids and flavonoids selectively extracted from Salvia lavandulifolia. With these non-volatile fractions, the thin-layer chromatographic separa...tions were carried out at ambient temperature (21 +/- 0.5 degrees C). Once again, we compared two variants of fractionation. A simpler one-dimensional variant consisted of the thin-layer chromatographic mode coupled with mass spectrometric fingerprinting of each separated fraction (1D TLC-MS). A more sophisticated variant was the two-dimensional liquid chromatographic system composed of the thin-layer chromatography and high-performance liquid chromatography, with mass spectrometric detection (2D TLC-LC-MS). As expected, the two-dimensional mode proved better performing than the one-dimensional mode (1D TLC-MS). It was concluded that thin-layer chromatography directly or indirectly coupled with mass spectrometric detection can prove very useful in the analysis of the phenolic acid and flavonoid fraction selectively extracted from botanical material.
Keywords:
flavonoids / mass spectrometric fingerprinting / phenolic acids / Salvia lavandulifolia / TLC-LC-MS / TLC-MSSource:
Journal of Liquid Chromatography and Related Technologies, 2011, 34, 10-11, 864-887Publisher:
- Taylor & Francis Inc, Philadelphia
Funding / projects:
- Sinteza, analiza i aktivnost novih organskih polidentatnih liganada i njihovih kompleksa sa d-metalima (RS-MESTD-MPN2006-2010-142062)
- European Social Fund (EFS) of the European Union
DOI: 10.1080/10826076.2011.571131
ISSN: 1082-6076
WoS: 000290991600009
Scopus: 2-s2.0-79957500585
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Sajewicz, Mieczyslaw AU - Staszek, Dorota AU - Natić, Maja AU - Wojtal, Lukasz AU - Waksmundzka-Hajnos, Monika AU - Kowalska, Teresa PY - 2011 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1348 AB - In the previous paper from this series, we proposed mass spectrometric fingerprinting of a complex and volatile botanical sample upon an example of the essential oil derived from Salvia lavandulifolia. In that paper, we compared two variants of fractionation of such a mixture. A simpler one-dimensional variant consisted of the low-temperature thin-layer chromatographic fractionation coupled with mass spectrometric fingerprinting of each separated fraction (1D LT TLC-MS). A more sophisticated variant was the two-dimensional liquid chromatographic system composed of the low-temperature thin-layer chromatography, high-performance liquid chromatography, and mass spectrometric detection (2D LT TLC-LC-MS). In this study, we present an analogous approach to the non-volatile botanical mixtures upon an example of the pharmacologically important phenolic acids and flavonoids selectively extracted from Salvia lavandulifolia. With these non-volatile fractions, the thin-layer chromatographic separations were carried out at ambient temperature (21 +/- 0.5 degrees C). Once again, we compared two variants of fractionation. A simpler one-dimensional variant consisted of the thin-layer chromatographic mode coupled with mass spectrometric fingerprinting of each separated fraction (1D TLC-MS). A more sophisticated variant was the two-dimensional liquid chromatographic system composed of the thin-layer chromatography and high-performance liquid chromatography, with mass spectrometric detection (2D TLC-LC-MS). As expected, the two-dimensional mode proved better performing than the one-dimensional mode (1D TLC-MS). It was concluded that thin-layer chromatography directly or indirectly coupled with mass spectrometric detection can prove very useful in the analysis of the phenolic acid and flavonoid fraction selectively extracted from botanical material. PB - Taylor & Francis Inc, Philadelphia T2 - Journal of Liquid Chromatography and Related Technologies T1 - Tlc-Ms Versus Tlc-Lc-Ms Fingerprints of Herbal Extracts. Part Ii. Phenolic Acids and Flavonoids VL - 34 IS - 10-11 SP - 864 EP - 887 DO - 10.1080/10826076.2011.571131 ER -
@article{ author = "Sajewicz, Mieczyslaw and Staszek, Dorota and Natić, Maja and Wojtal, Lukasz and Waksmundzka-Hajnos, Monika and Kowalska, Teresa", year = "2011", abstract = "In the previous paper from this series, we proposed mass spectrometric fingerprinting of a complex and volatile botanical sample upon an example of the essential oil derived from Salvia lavandulifolia. In that paper, we compared two variants of fractionation of such a mixture. A simpler one-dimensional variant consisted of the low-temperature thin-layer chromatographic fractionation coupled with mass spectrometric fingerprinting of each separated fraction (1D LT TLC-MS). A more sophisticated variant was the two-dimensional liquid chromatographic system composed of the low-temperature thin-layer chromatography, high-performance liquid chromatography, and mass spectrometric detection (2D LT TLC-LC-MS). In this study, we present an analogous approach to the non-volatile botanical mixtures upon an example of the pharmacologically important phenolic acids and flavonoids selectively extracted from Salvia lavandulifolia. With these non-volatile fractions, the thin-layer chromatographic separations were carried out at ambient temperature (21 +/- 0.5 degrees C). Once again, we compared two variants of fractionation. A simpler one-dimensional variant consisted of the thin-layer chromatographic mode coupled with mass spectrometric fingerprinting of each separated fraction (1D TLC-MS). A more sophisticated variant was the two-dimensional liquid chromatographic system composed of the thin-layer chromatography and high-performance liquid chromatography, with mass spectrometric detection (2D TLC-LC-MS). As expected, the two-dimensional mode proved better performing than the one-dimensional mode (1D TLC-MS). It was concluded that thin-layer chromatography directly or indirectly coupled with mass spectrometric detection can prove very useful in the analysis of the phenolic acid and flavonoid fraction selectively extracted from botanical material.", publisher = "Taylor & Francis Inc, Philadelphia", journal = "Journal of Liquid Chromatography and Related Technologies", title = "Tlc-Ms Versus Tlc-Lc-Ms Fingerprints of Herbal Extracts. Part Ii. Phenolic Acids and Flavonoids", volume = "34", number = "10-11", pages = "864-887", doi = "10.1080/10826076.2011.571131" }
Sajewicz, M., Staszek, D., Natić, M., Wojtal, L., Waksmundzka-Hajnos, M.,& Kowalska, T.. (2011). Tlc-Ms Versus Tlc-Lc-Ms Fingerprints of Herbal Extracts. Part Ii. Phenolic Acids and Flavonoids. in Journal of Liquid Chromatography and Related Technologies Taylor & Francis Inc, Philadelphia., 34(10-11), 864-887. https://doi.org/10.1080/10826076.2011.571131
Sajewicz M, Staszek D, Natić M, Wojtal L, Waksmundzka-Hajnos M, Kowalska T. Tlc-Ms Versus Tlc-Lc-Ms Fingerprints of Herbal Extracts. Part Ii. Phenolic Acids and Flavonoids. in Journal of Liquid Chromatography and Related Technologies. 2011;34(10-11):864-887. doi:10.1080/10826076.2011.571131 .
Sajewicz, Mieczyslaw, Staszek, Dorota, Natić, Maja, Wojtal, Lukasz, Waksmundzka-Hajnos, Monika, Kowalska, Teresa, "Tlc-Ms Versus Tlc-Lc-Ms Fingerprints of Herbal Extracts. Part Ii. Phenolic Acids and Flavonoids" in Journal of Liquid Chromatography and Related Technologies, 34, no. 10-11 (2011):864-887, https://doi.org/10.1080/10826076.2011.571131 . .