Limited Aromatic Pathway Genes Diversity Amongst Aromatic Compound Degrading Soil Bacterial Isolates
2013
Authors
Minovska, GordanaNarančić, Tanja
Mandić, Mina
Šenerović, Lidija
Vasiljević, Branka
Nikodinović-Runić, Jasmina
Article (Published version)
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Identification and characterization of novel genes belonging to microbial aromatic biodegradation pathway is of great importance as they have been proven versatile biocatalysts. In this study, the selection of 19 environmental bacterial isolates capable to degrade a wide range of aromatic compounds has been screened for the presence of five genes from the lower and the upper aromatic biodegradation pathway using PCR methodology. In the case of 4-oxalocrotonate tautomerase and toluene dioxygenases, although present in the most of environmental isolates, very limited diversity of the genes has been encountered. Highly conserved sequences of these genes in environmental samples revealed high homology with gene sequences of the characterised corresponding genes from Pseudomonas putida species. The screen using degenerate primers based on known catechol-and naphthalene dioxygenases sequences resulted in a limited number of amplified fragments. Only two catechol 2,3-dioxygenase from two Baci...llus isolates were amplified and showed no significant similarities with dioxygenases from characterized organisms, but 80-90% identities with partial catechol 2,3-dioxygenase sequences from uncultured organisms. Potentially three novel catechol 1,2-dioxygenases were identified from Bacillus sp. TN102, Gordonia sp. TN103 and Rhodococcus sp. TN112. Highly homologous tautomerase and toluene dioxygenases amongst environmental samples isolated from the contaminated environment suggested horizontal gene transfer while limited success in PCR detection of the other three genes indicates that these isolates may still be a source of novel genes.
Keywords:
Biodegradation / aromatic compounds / 4-oxalocrotonate tautomeraseSource:
Genetika (Beograd), 2013, 45, 3, 703-716Publisher:
- Serbian Genetics Soc, Belgrade
Funding / projects:
- Microbial diversity study and characterization of beneficial environmental microorganisms (RS-MESTD-Basic Research (BR or ON)-173048)
DOI: 10.2298/GENSR1303703M
ISSN: 0534-0012
WoS: 000330186600008
Scopus: 2-s2.0-84894584979
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Inovacioni centar / Innovation CentreTY - JOUR AU - Minovska, Gordana AU - Narančić, Tanja AU - Mandić, Mina AU - Šenerović, Lidija AU - Vasiljević, Branka AU - Nikodinović-Runić, Jasmina PY - 2013 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1493 AB - Identification and characterization of novel genes belonging to microbial aromatic biodegradation pathway is of great importance as they have been proven versatile biocatalysts. In this study, the selection of 19 environmental bacterial isolates capable to degrade a wide range of aromatic compounds has been screened for the presence of five genes from the lower and the upper aromatic biodegradation pathway using PCR methodology. In the case of 4-oxalocrotonate tautomerase and toluene dioxygenases, although present in the most of environmental isolates, very limited diversity of the genes has been encountered. Highly conserved sequences of these genes in environmental samples revealed high homology with gene sequences of the characterised corresponding genes from Pseudomonas putida species. The screen using degenerate primers based on known catechol-and naphthalene dioxygenases sequences resulted in a limited number of amplified fragments. Only two catechol 2,3-dioxygenase from two Bacillus isolates were amplified and showed no significant similarities with dioxygenases from characterized organisms, but 80-90% identities with partial catechol 2,3-dioxygenase sequences from uncultured organisms. Potentially three novel catechol 1,2-dioxygenases were identified from Bacillus sp. TN102, Gordonia sp. TN103 and Rhodococcus sp. TN112. Highly homologous tautomerase and toluene dioxygenases amongst environmental samples isolated from the contaminated environment suggested horizontal gene transfer while limited success in PCR detection of the other three genes indicates that these isolates may still be a source of novel genes. PB - Serbian Genetics Soc, Belgrade T2 - Genetika (Beograd) T1 - Limited Aromatic Pathway Genes Diversity Amongst Aromatic Compound Degrading Soil Bacterial Isolates VL - 45 IS - 3 SP - 703 EP - 716 DO - 10.2298/GENSR1303703M ER -
@article{ author = "Minovska, Gordana and Narančić, Tanja and Mandić, Mina and Šenerović, Lidija and Vasiljević, Branka and Nikodinović-Runić, Jasmina", year = "2013", abstract = "Identification and characterization of novel genes belonging to microbial aromatic biodegradation pathway is of great importance as they have been proven versatile biocatalysts. In this study, the selection of 19 environmental bacterial isolates capable to degrade a wide range of aromatic compounds has been screened for the presence of five genes from the lower and the upper aromatic biodegradation pathway using PCR methodology. In the case of 4-oxalocrotonate tautomerase and toluene dioxygenases, although present in the most of environmental isolates, very limited diversity of the genes has been encountered. Highly conserved sequences of these genes in environmental samples revealed high homology with gene sequences of the characterised corresponding genes from Pseudomonas putida species. The screen using degenerate primers based on known catechol-and naphthalene dioxygenases sequences resulted in a limited number of amplified fragments. Only two catechol 2,3-dioxygenase from two Bacillus isolates were amplified and showed no significant similarities with dioxygenases from characterized organisms, but 80-90% identities with partial catechol 2,3-dioxygenase sequences from uncultured organisms. Potentially three novel catechol 1,2-dioxygenases were identified from Bacillus sp. TN102, Gordonia sp. TN103 and Rhodococcus sp. TN112. Highly homologous tautomerase and toluene dioxygenases amongst environmental samples isolated from the contaminated environment suggested horizontal gene transfer while limited success in PCR detection of the other three genes indicates that these isolates may still be a source of novel genes.", publisher = "Serbian Genetics Soc, Belgrade", journal = "Genetika (Beograd)", title = "Limited Aromatic Pathway Genes Diversity Amongst Aromatic Compound Degrading Soil Bacterial Isolates", volume = "45", number = "3", pages = "703-716", doi = "10.2298/GENSR1303703M" }
Minovska, G., Narančić, T., Mandić, M., Šenerović, L., Vasiljević, B.,& Nikodinović-Runić, J.. (2013). Limited Aromatic Pathway Genes Diversity Amongst Aromatic Compound Degrading Soil Bacterial Isolates. in Genetika (Beograd) Serbian Genetics Soc, Belgrade., 45(3), 703-716. https://doi.org/10.2298/GENSR1303703M
Minovska G, Narančić T, Mandić M, Šenerović L, Vasiljević B, Nikodinović-Runić J. Limited Aromatic Pathway Genes Diversity Amongst Aromatic Compound Degrading Soil Bacterial Isolates. in Genetika (Beograd). 2013;45(3):703-716. doi:10.2298/GENSR1303703M .
Minovska, Gordana, Narančić, Tanja, Mandić, Mina, Šenerović, Lidija, Vasiljević, Branka, Nikodinović-Runić, Jasmina, "Limited Aromatic Pathway Genes Diversity Amongst Aromatic Compound Degrading Soil Bacterial Isolates" in Genetika (Beograd), 45, no. 3 (2013):703-716, https://doi.org/10.2298/GENSR1303703M . .