Comparative analysis of antioxidant activity of honey of different floral sources using recently developed polarographic and various spectrophotometric assays
Samo za registrovane korisnike
2013
Autori
Gorjanović, StanislavaAlvarez-Suarez, Jose Miguel
Novaković, Miroslav M.
Pastor, Ferenc
Pezo, Lato
Battino, Maurizio
Suznjevic, Desanka Z.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Hydrogen peroxide scavenging (HPS) activity of honey of different floral sources and its constituents such as predominant honey flavonoids, phenolic acids, amino and organic acids, and carbohydrates have been assessed by direct current (DC) polarographic assay. The assay was based on decrease of anodic current of hydrogen peroxide complex, formed in alkaline solution, at the potential of mercury dissolution. High correlations between honey HPS activity, its total phenolic content (FC-GAE), antioxidant activity measured by four standard methods (DPPH, TEAC, FRAP and ORAC), and also the relative antioxidant capacity index, were obtained. Statistical evaluation by ANOVA and F-test further confirmed the assay validity. The results for individual compounds showed that HPS activity of honey reflects an integrated action of a wide range of constituents, both phenolics and non-phenolics. The polarographic assay applied is a fast, reliable and low cost alternative to spectrophotometric antioxid...ant assays commonly applied in analysis of honey and can serve as an indicator of honey quality.
Ključne reči:
Analytical method / Food analysis / Honey / Honey constituents / Food composition / Phenolics / Reducing sugars / Antioxidant activity / Hydrogen peroxide scavenge / DC polarography / Anodic current / Honey adulIzvor:
Journal of Food Composition and Analysis, 2013, 30, 1, 13-18Izdavač:
- Academic Press Inc Elsevier Science, San Diego
Finansiranje / projekti:
- Modifikacije antioksidativnog metabolizma biljaka sa ciljem povećanja tolerancije na abiotski stres i identifikacija novih biomarkera sa primenom u remedijaciji i monitoringu degradiranih staništa (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43010)
- Uticaj sorte i uslova gajenja na sadržaj bioaktivnih komponenti jagodastog i koštičavog voća i dobijanje biološki vrednih proizvoda poboljšanim i novim tehnologijama (RS-MESTD-Technological Development (TD or TR)-31093)
DOI: 10.1016/j.jfca.2012.12.004
ISSN: 0889-1575
WoS: 000317799500003
Scopus: 2-s2.0-84873531795
Kolekcije
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Gorjanović, Stanislava AU - Alvarez-Suarez, Jose Miguel AU - Novaković, Miroslav M. AU - Pastor, Ferenc AU - Pezo, Lato AU - Battino, Maurizio AU - Suznjevic, Desanka Z. PY - 2013 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1629 AB - Hydrogen peroxide scavenging (HPS) activity of honey of different floral sources and its constituents such as predominant honey flavonoids, phenolic acids, amino and organic acids, and carbohydrates have been assessed by direct current (DC) polarographic assay. The assay was based on decrease of anodic current of hydrogen peroxide complex, formed in alkaline solution, at the potential of mercury dissolution. High correlations between honey HPS activity, its total phenolic content (FC-GAE), antioxidant activity measured by four standard methods (DPPH, TEAC, FRAP and ORAC), and also the relative antioxidant capacity index, were obtained. Statistical evaluation by ANOVA and F-test further confirmed the assay validity. The results for individual compounds showed that HPS activity of honey reflects an integrated action of a wide range of constituents, both phenolics and non-phenolics. The polarographic assay applied is a fast, reliable and low cost alternative to spectrophotometric antioxidant assays commonly applied in analysis of honey and can serve as an indicator of honey quality. PB - Academic Press Inc Elsevier Science, San Diego T2 - Journal of Food Composition and Analysis T1 - Comparative analysis of antioxidant activity of honey of different floral sources using recently developed polarographic and various spectrophotometric assays VL - 30 IS - 1 SP - 13 EP - 18 DO - 10.1016/j.jfca.2012.12.004 ER -
@article{ author = "Gorjanović, Stanislava and Alvarez-Suarez, Jose Miguel and Novaković, Miroslav M. and Pastor, Ferenc and Pezo, Lato and Battino, Maurizio and Suznjevic, Desanka Z.", year = "2013", abstract = "Hydrogen peroxide scavenging (HPS) activity of honey of different floral sources and its constituents such as predominant honey flavonoids, phenolic acids, amino and organic acids, and carbohydrates have been assessed by direct current (DC) polarographic assay. The assay was based on decrease of anodic current of hydrogen peroxide complex, formed in alkaline solution, at the potential of mercury dissolution. High correlations between honey HPS activity, its total phenolic content (FC-GAE), antioxidant activity measured by four standard methods (DPPH, TEAC, FRAP and ORAC), and also the relative antioxidant capacity index, were obtained. Statistical evaluation by ANOVA and F-test further confirmed the assay validity. The results for individual compounds showed that HPS activity of honey reflects an integrated action of a wide range of constituents, both phenolics and non-phenolics. The polarographic assay applied is a fast, reliable and low cost alternative to spectrophotometric antioxidant assays commonly applied in analysis of honey and can serve as an indicator of honey quality.", publisher = "Academic Press Inc Elsevier Science, San Diego", journal = "Journal of Food Composition and Analysis", title = "Comparative analysis of antioxidant activity of honey of different floral sources using recently developed polarographic and various spectrophotometric assays", volume = "30", number = "1", pages = "13-18", doi = "10.1016/j.jfca.2012.12.004" }
Gorjanović, S., Alvarez-Suarez, J. M., Novaković, M. M., Pastor, F., Pezo, L., Battino, M.,& Suznjevic, D. Z.. (2013). Comparative analysis of antioxidant activity of honey of different floral sources using recently developed polarographic and various spectrophotometric assays. in Journal of Food Composition and Analysis Academic Press Inc Elsevier Science, San Diego., 30(1), 13-18. https://doi.org/10.1016/j.jfca.2012.12.004
Gorjanović S, Alvarez-Suarez JM, Novaković MM, Pastor F, Pezo L, Battino M, Suznjevic DZ. Comparative analysis of antioxidant activity of honey of different floral sources using recently developed polarographic and various spectrophotometric assays. in Journal of Food Composition and Analysis. 2013;30(1):13-18. doi:10.1016/j.jfca.2012.12.004 .
Gorjanović, Stanislava, Alvarez-Suarez, Jose Miguel, Novaković, Miroslav M., Pastor, Ferenc, Pezo, Lato, Battino, Maurizio, Suznjevic, Desanka Z., "Comparative analysis of antioxidant activity of honey of different floral sources using recently developed polarographic and various spectrophotometric assays" in Journal of Food Composition and Analysis, 30, no. 1 (2013):13-18, https://doi.org/10.1016/j.jfca.2012.12.004 . .