How the sialylation level of serum N-acetyl-beta-D-glucosaminidase A form in Type 1 diabetes mellitus influences their activity?
Abstract
It was verified that the serum N-acetyl-beta-D-glucosaminidase (NAG) activity is elevated in diabetes, but there are no reports about changes in the sialic acid (SA) content in the carbohydrate parts of the NAG A form and its influence on the total changes in NAG activity in type 1 diabetes mellitus patients with and without secondary complications. The NAG A form was isolated from the serum of 81 insulin-dependent diabetes mellitus (IDDM) patients with and without secondary complications (retinopathy, polyneuropathy and nephropathy) and 25 healthy persons, and purified and characterised. The content of alpha-2,6-bound SA, the isoenzyme patterns of the purified A form, and the total NAG and A form activities were determined. In all diabetic groups, the sialylation levels of the A form were 2-3.5 times lower compared to control, while their acidities (fractions with pI 4.25-5.1) increased, particularly with progression of secondary complications. Total serum NAG activities and percentag...es of A form were significantly higher (P lt 0.001) in all diabetic groups and negatively correlated with the alpha-2,6-bound SA content of the A form. In addition, they decreased as secondary diabetic complications became more complex. The observed changes could be the consequence of structural changes in the A form due to significant increases in its acidity, i.e., negative charge, which originated from groups other than SA.
Keywords:
N-acetyl-beta-D-glucosaminidase / A isoenzyme isolation and characterization / sialylation level, diabetes mellitus type 1 / secondary complicationsSource:
Journal of the Serbian Chemical Society, 2014, 79, 12, 1491-1503Publisher:
- Serbian Chemical Soc, Belgrade
Funding / projects:
DOI: 10.2298/JSC140430076J
ISSN: 0352-5139
WoS: 000348749400004
Scopus: 2-s2.0-84920867009
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Jovanović, Vesna B. AU - Aćimović, Jelena M. AU - Sreckovic, Vesna S. Dimitrijevic AU - Mandić, Ljuba M. PY - 2014 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1647 AB - It was verified that the serum N-acetyl-beta-D-glucosaminidase (NAG) activity is elevated in diabetes, but there are no reports about changes in the sialic acid (SA) content in the carbohydrate parts of the NAG A form and its influence on the total changes in NAG activity in type 1 diabetes mellitus patients with and without secondary complications. The NAG A form was isolated from the serum of 81 insulin-dependent diabetes mellitus (IDDM) patients with and without secondary complications (retinopathy, polyneuropathy and nephropathy) and 25 healthy persons, and purified and characterised. The content of alpha-2,6-bound SA, the isoenzyme patterns of the purified A form, and the total NAG and A form activities were determined. In all diabetic groups, the sialylation levels of the A form were 2-3.5 times lower compared to control, while their acidities (fractions with pI 4.25-5.1) increased, particularly with progression of secondary complications. Total serum NAG activities and percentages of A form were significantly higher (P lt 0.001) in all diabetic groups and negatively correlated with the alpha-2,6-bound SA content of the A form. In addition, they decreased as secondary diabetic complications became more complex. The observed changes could be the consequence of structural changes in the A form due to significant increases in its acidity, i.e., negative charge, which originated from groups other than SA. PB - Serbian Chemical Soc, Belgrade T2 - Journal of the Serbian Chemical Society T1 - How the sialylation level of serum N-acetyl-beta-D-glucosaminidase A form in Type 1 diabetes mellitus influences their activity? VL - 79 IS - 12 SP - 1491 EP - 1503 DO - 10.2298/JSC140430076J ER -
@article{ author = "Jovanović, Vesna B. and Aćimović, Jelena M. and Sreckovic, Vesna S. Dimitrijevic and Mandić, Ljuba M.", year = "2014", abstract = "It was verified that the serum N-acetyl-beta-D-glucosaminidase (NAG) activity is elevated in diabetes, but there are no reports about changes in the sialic acid (SA) content in the carbohydrate parts of the NAG A form and its influence on the total changes in NAG activity in type 1 diabetes mellitus patients with and without secondary complications. The NAG A form was isolated from the serum of 81 insulin-dependent diabetes mellitus (IDDM) patients with and without secondary complications (retinopathy, polyneuropathy and nephropathy) and 25 healthy persons, and purified and characterised. The content of alpha-2,6-bound SA, the isoenzyme patterns of the purified A form, and the total NAG and A form activities were determined. In all diabetic groups, the sialylation levels of the A form were 2-3.5 times lower compared to control, while their acidities (fractions with pI 4.25-5.1) increased, particularly with progression of secondary complications. Total serum NAG activities and percentages of A form were significantly higher (P lt 0.001) in all diabetic groups and negatively correlated with the alpha-2,6-bound SA content of the A form. In addition, they decreased as secondary diabetic complications became more complex. The observed changes could be the consequence of structural changes in the A form due to significant increases in its acidity, i.e., negative charge, which originated from groups other than SA.", publisher = "Serbian Chemical Soc, Belgrade", journal = "Journal of the Serbian Chemical Society", title = "How the sialylation level of serum N-acetyl-beta-D-glucosaminidase A form in Type 1 diabetes mellitus influences their activity?", volume = "79", number = "12", pages = "1491-1503", doi = "10.2298/JSC140430076J" }
Jovanović, V. B., Aćimović, J. M., Sreckovic, V. S. D.,& Mandić, L. M.. (2014). How the sialylation level of serum N-acetyl-beta-D-glucosaminidase A form in Type 1 diabetes mellitus influences their activity?. in Journal of the Serbian Chemical Society Serbian Chemical Soc, Belgrade., 79(12), 1491-1503. https://doi.org/10.2298/JSC140430076J
Jovanović VB, Aćimović JM, Sreckovic VSD, Mandić LM. How the sialylation level of serum N-acetyl-beta-D-glucosaminidase A form in Type 1 diabetes mellitus influences their activity?. in Journal of the Serbian Chemical Society. 2014;79(12):1491-1503. doi:10.2298/JSC140430076J .
Jovanović, Vesna B., Aćimović, Jelena M., Sreckovic, Vesna S. Dimitrijevic, Mandić, Ljuba M., "How the sialylation level of serum N-acetyl-beta-D-glucosaminidase A form in Type 1 diabetes mellitus influences their activity?" in Journal of the Serbian Chemical Society, 79, no. 12 (2014):1491-1503, https://doi.org/10.2298/JSC140430076J . .