Приказ основних података о документу
How the sialylation level of serum N-acetyl-beta-D-glucosaminidase A form in Type 1 diabetes mellitus influences their activity?
dc.creator | Jovanović, Vesna B. | |
dc.creator | Aćimović, Jelena M. | |
dc.creator | Sreckovic, Vesna S. Dimitrijevic | |
dc.creator | Mandić, Ljuba M. | |
dc.date.accessioned | 2018-11-22T00:27:30Z | |
dc.date.available | 2018-11-22T00:27:30Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 0352-5139 | |
dc.identifier.uri | https://cherry.chem.bg.ac.rs/handle/123456789/1647 | |
dc.description.abstract | It was verified that the serum N-acetyl-beta-D-glucosaminidase (NAG) activity is elevated in diabetes, but there are no reports about changes in the sialic acid (SA) content in the carbohydrate parts of the NAG A form and its influence on the total changes in NAG activity in type 1 diabetes mellitus patients with and without secondary complications. The NAG A form was isolated from the serum of 81 insulin-dependent diabetes mellitus (IDDM) patients with and without secondary complications (retinopathy, polyneuropathy and nephropathy) and 25 healthy persons, and purified and characterised. The content of alpha-2,6-bound SA, the isoenzyme patterns of the purified A form, and the total NAG and A form activities were determined. In all diabetic groups, the sialylation levels of the A form were 2-3.5 times lower compared to control, while their acidities (fractions with pI 4.25-5.1) increased, particularly with progression of secondary complications. Total serum NAG activities and percentages of A form were significantly higher (P lt 0.001) in all diabetic groups and negatively correlated with the alpha-2,6-bound SA content of the A form. In addition, they decreased as secondary diabetic complications became more complex. The observed changes could be the consequence of structural changes in the A form due to significant increases in its acidity, i.e., negative charge, which originated from groups other than SA. | en |
dc.publisher | Serbian Chemical Soc, Belgrade | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Journal of the Serbian Chemical Society | |
dc.subject | N-acetyl-beta-D-glucosaminidase | en |
dc.subject | A isoenzyme isolation and characterization | en |
dc.subject | sialylation level, diabetes mellitus type 1 | en |
dc.subject | secondary complications | en |
dc.title | How the sialylation level of serum N-acetyl-beta-D-glucosaminidase A form in Type 1 diabetes mellitus influences their activity? | en |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dcterms.abstract | Срецковиц, Весна С. Димитријевиц; Aћимовић, Јелена; Мандић, Љуба М.; Јовановић, Весна; | |
dc.citation.volume | 79 | |
dc.citation.issue | 12 | |
dc.citation.spage | 1491 | |
dc.citation.epage | 1503 | |
dc.identifier.wos | 000348749400004 | |
dc.identifier.doi | 10.2298/JSC140430076J | |
dc.citation.other | 79(12): 1491-1503 | |
dc.citation.rank | M23 | |
dc.type.version | publishedVersion | |
dc.identifier.scopus | 2-s2.0-84920867009 | |
dc.identifier.fulltext | https://cherry.chem.bg.ac.rs/bitstream/id/8729/1645.pdf |