The Immunosuppressive drug - Rapamycin - Electroanalytical Sensing Using Boron-Doped Diamond electrode
Abstract
This paper presents for the first time the study of electrochemical behavior of well known immunosuppressant drug - rapamycin (sirolimus) using boron-doped diamond electrode. Rapamycin provided single and oval-shaped oxidation peak at +1.1 V vs.Ag/AgCl electrode in Britton-Robinson buffer solution at pH 3 confirming highly irreversible behavior of analyte at boron-doped diamond electrode. A differential pulse voltammetry was used for quantification of tested drug under the optimum experimental conditions. The calibration curve was linear over the range from 0.5 to 19.5 mu M (R-2 = 0.9976) with detection limit of 0.22 mu M. Repeatability of ten successfully measurements of three different concentrations (5,10 and 15 mu M) was 2.5, 1.9 and 1.7%, respectively. Influence of most common biomolecules presented in urine samples was evaluated. The suggested analytical methodology was successfully applied for determination of rapamycin in four urine samples with excellent recoveries. The develo...ped approach could be beneficial in analysis of rapamycin in biological samples using boron-doped diamond electrode as up-to-date electrochemical sensor and could represent inexpensive analytical alternative to separation methods.
Keywords:
rapamycin / differential pulse voltammetry / boron-doped diamond electrode / electrochemical oxidationSource:
Electrochimica Acta, 2015, 168, 76-81Publisher:
- Pergamon-Elsevier Science Ltd, Oxford
Funding / projects:
- Application of advanced oxidation processes and nanostructured oxide materials for the removal of pollutants from the environment, development and optimisation of instrumental techniques for efficiency monitoring (RS-MESTD-Basic Research (BR or ON)-172030)
DOI: 10.1016/j.electacta.2015.03.200
ISSN: 0013-4686
WoS: 000354055500010
Scopus: 2-s2.0-84926507615
Collections
Institution/Community
Inovacioni centar / Innovation CentreTY - JOUR AU - Stanković, Dalibor AU - Katcher, Kurt PY - 2015 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1707 AB - This paper presents for the first time the study of electrochemical behavior of well known immunosuppressant drug - rapamycin (sirolimus) using boron-doped diamond electrode. Rapamycin provided single and oval-shaped oxidation peak at +1.1 V vs.Ag/AgCl electrode in Britton-Robinson buffer solution at pH 3 confirming highly irreversible behavior of analyte at boron-doped diamond electrode. A differential pulse voltammetry was used for quantification of tested drug under the optimum experimental conditions. The calibration curve was linear over the range from 0.5 to 19.5 mu M (R-2 = 0.9976) with detection limit of 0.22 mu M. Repeatability of ten successfully measurements of three different concentrations (5,10 and 15 mu M) was 2.5, 1.9 and 1.7%, respectively. Influence of most common biomolecules presented in urine samples was evaluated. The suggested analytical methodology was successfully applied for determination of rapamycin in four urine samples with excellent recoveries. The developed approach could be beneficial in analysis of rapamycin in biological samples using boron-doped diamond electrode as up-to-date electrochemical sensor and could represent inexpensive analytical alternative to separation methods. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Electrochimica Acta T1 - The Immunosuppressive drug - Rapamycin - Electroanalytical Sensing Using Boron-Doped Diamond electrode VL - 168 SP - 76 EP - 81 DO - 10.1016/j.electacta.2015.03.200 ER -
@article{ author = "Stanković, Dalibor and Katcher, Kurt", year = "2015", abstract = "This paper presents for the first time the study of electrochemical behavior of well known immunosuppressant drug - rapamycin (sirolimus) using boron-doped diamond electrode. Rapamycin provided single and oval-shaped oxidation peak at +1.1 V vs.Ag/AgCl electrode in Britton-Robinson buffer solution at pH 3 confirming highly irreversible behavior of analyte at boron-doped diamond electrode. A differential pulse voltammetry was used for quantification of tested drug under the optimum experimental conditions. The calibration curve was linear over the range from 0.5 to 19.5 mu M (R-2 = 0.9976) with detection limit of 0.22 mu M. Repeatability of ten successfully measurements of three different concentrations (5,10 and 15 mu M) was 2.5, 1.9 and 1.7%, respectively. Influence of most common biomolecules presented in urine samples was evaluated. The suggested analytical methodology was successfully applied for determination of rapamycin in four urine samples with excellent recoveries. The developed approach could be beneficial in analysis of rapamycin in biological samples using boron-doped diamond electrode as up-to-date electrochemical sensor and could represent inexpensive analytical alternative to separation methods.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Electrochimica Acta", title = "The Immunosuppressive drug - Rapamycin - Electroanalytical Sensing Using Boron-Doped Diamond electrode", volume = "168", pages = "76-81", doi = "10.1016/j.electacta.2015.03.200" }
Stanković, D.,& Katcher, K.. (2015). The Immunosuppressive drug - Rapamycin - Electroanalytical Sensing Using Boron-Doped Diamond electrode. in Electrochimica Acta Pergamon-Elsevier Science Ltd, Oxford., 168, 76-81. https://doi.org/10.1016/j.electacta.2015.03.200
Stanković D, Katcher K. The Immunosuppressive drug - Rapamycin - Electroanalytical Sensing Using Boron-Doped Diamond electrode. in Electrochimica Acta. 2015;168:76-81. doi:10.1016/j.electacta.2015.03.200 .
Stanković, Dalibor, Katcher, Kurt, "The Immunosuppressive drug - Rapamycin - Electroanalytical Sensing Using Boron-Doped Diamond electrode" in Electrochimica Acta, 168 (2015):76-81, https://doi.org/10.1016/j.electacta.2015.03.200 . .