Mapping electrogenerated chemiluminescence reactivity in space: mechanistic insight into model systems used in immunoassays
Само за регистроване кориснике
2014
Аутори
Sentić, MilicaMilutinovic, Milena
Kanoufi, Frederic
Manojlović, Dragan D.
Arbault, Stephane
Šojić, Nešo
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The remarkable characteristics of electrogenerated chemiluminescence (ECL) as a readout method are successfully exploited in numerous microbead-based immunoassays. However there is still a lack of understanding of the extremely high sensitivity of such ECL bioassays. Here the mechanisms of the reaction of the Ru(bpy)(3)(2+) luminophore with two efficient co-reactants (TPrA or DBAE) were investigated by mapping the ECL reactivity at the level of single Ru(bpy)(3)(2+)-functionalized beads. Micrometric non-conductive beads were decorated with the ruthenium label via a sandwich immunoassay or via a peptide bond. Mapping the ECL reactivity on one bead demonstrates the generation of the excited state at a micrometric distance from the electrode by reaction of surface-confined Ru(bpy)(3)(2+) with diffusing TPrA radicals. The signature of the TPAc center dot+ lifetime is obtained from the ECL profile. Unlike the reaction with Ru(bpy)(3)(2+) in solution, DBAE generates very low ECL intensity in... the bead-based format suggesting more unstable radical intermediates. The 3D imaging approach provides insights into the ECL mechanistic route operating in bioassays and on the optical effects that focus the ECL emission.
Извор:
Chemical Science, 2014, 5, 6, 2568-2572Издавач:
- Royal Soc Chemistry, Cambridge
Финансирање / пројекти:
- French Foreign Ministry
- Ministry of Science and Technological Development (Republic of Serbia)
DOI: 10.1039/c4sc00312h
ISSN: 2041-6520
WoS: 000336254500061
Scopus: 2-s2.0-84900311148
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Sentić, Milica AU - Milutinovic, Milena AU - Kanoufi, Frederic AU - Manojlović, Dragan D. AU - Arbault, Stephane AU - Šojić, Nešo PY - 2014 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1779 AB - The remarkable characteristics of electrogenerated chemiluminescence (ECL) as a readout method are successfully exploited in numerous microbead-based immunoassays. However there is still a lack of understanding of the extremely high sensitivity of such ECL bioassays. Here the mechanisms of the reaction of the Ru(bpy)(3)(2+) luminophore with two efficient co-reactants (TPrA or DBAE) were investigated by mapping the ECL reactivity at the level of single Ru(bpy)(3)(2+)-functionalized beads. Micrometric non-conductive beads were decorated with the ruthenium label via a sandwich immunoassay or via a peptide bond. Mapping the ECL reactivity on one bead demonstrates the generation of the excited state at a micrometric distance from the electrode by reaction of surface-confined Ru(bpy)(3)(2+) with diffusing TPrA radicals. The signature of the TPAc center dot+ lifetime is obtained from the ECL profile. Unlike the reaction with Ru(bpy)(3)(2+) in solution, DBAE generates very low ECL intensity in the bead-based format suggesting more unstable radical intermediates. The 3D imaging approach provides insights into the ECL mechanistic route operating in bioassays and on the optical effects that focus the ECL emission. PB - Royal Soc Chemistry, Cambridge T2 - Chemical Science T1 - Mapping electrogenerated chemiluminescence reactivity in space: mechanistic insight into model systems used in immunoassays VL - 5 IS - 6 SP - 2568 EP - 2572 DO - 10.1039/c4sc00312h ER -
@article{ author = "Sentić, Milica and Milutinovic, Milena and Kanoufi, Frederic and Manojlović, Dragan D. and Arbault, Stephane and Šojić, Nešo", year = "2014", abstract = "The remarkable characteristics of electrogenerated chemiluminescence (ECL) as a readout method are successfully exploited in numerous microbead-based immunoassays. However there is still a lack of understanding of the extremely high sensitivity of such ECL bioassays. Here the mechanisms of the reaction of the Ru(bpy)(3)(2+) luminophore with two efficient co-reactants (TPrA or DBAE) were investigated by mapping the ECL reactivity at the level of single Ru(bpy)(3)(2+)-functionalized beads. Micrometric non-conductive beads were decorated with the ruthenium label via a sandwich immunoassay or via a peptide bond. Mapping the ECL reactivity on one bead demonstrates the generation of the excited state at a micrometric distance from the electrode by reaction of surface-confined Ru(bpy)(3)(2+) with diffusing TPrA radicals. The signature of the TPAc center dot+ lifetime is obtained from the ECL profile. Unlike the reaction with Ru(bpy)(3)(2+) in solution, DBAE generates very low ECL intensity in the bead-based format suggesting more unstable radical intermediates. The 3D imaging approach provides insights into the ECL mechanistic route operating in bioassays and on the optical effects that focus the ECL emission.", publisher = "Royal Soc Chemistry, Cambridge", journal = "Chemical Science", title = "Mapping electrogenerated chemiluminescence reactivity in space: mechanistic insight into model systems used in immunoassays", volume = "5", number = "6", pages = "2568-2572", doi = "10.1039/c4sc00312h" }
Sentić, M., Milutinovic, M., Kanoufi, F., Manojlović, D. D., Arbault, S.,& Šojić, N.. (2014). Mapping electrogenerated chemiluminescence reactivity in space: mechanistic insight into model systems used in immunoassays. in Chemical Science Royal Soc Chemistry, Cambridge., 5(6), 2568-2572. https://doi.org/10.1039/c4sc00312h
Sentić M, Milutinovic M, Kanoufi F, Manojlović DD, Arbault S, Šojić N. Mapping electrogenerated chemiluminescence reactivity in space: mechanistic insight into model systems used in immunoassays. in Chemical Science. 2014;5(6):2568-2572. doi:10.1039/c4sc00312h .
Sentić, Milica, Milutinovic, Milena, Kanoufi, Frederic, Manojlović, Dragan D., Arbault, Stephane, Šojić, Nešo, "Mapping electrogenerated chemiluminescence reactivity in space: mechanistic insight into model systems used in immunoassays" in Chemical Science, 5, no. 6 (2014):2568-2572, https://doi.org/10.1039/c4sc00312h . .