Antioxidative responses of the tissues of two wild populations of Pelophylax kl. esculentus frogs to heavy metal pollution
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2016
Authors
Prokić, Marko D.Borković-Mitić, Slavica S.
Krizmanić, Imre I.
Mutić, Jelena
Vukojević, Vesna
Nasia, Mohammed
Gavrić, Jelena P.
Despotović, Svetlana G.
Gavrilović, Branka R.
Radovanović, Tijana B.
Pavlović, Slađan Z.
Saičić, Zorica S.
Article (Published version)
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Heavy metal pollution of the aquatic environment is of great concern worldwide. Heavy metals are capable of inducing oxidative stress by increasing the formation of reactive oxygen species (ROS), and directly affecting the antioxidant defense system (AOS) in living organisms. The frog Pelophylax kl. esculentus is a semiaquatic species with semipermeable skin and a complex lifecycle, and represents a potentially useful bioindicator organism. The aim of this study was to investigate the accumulation of several heavy metals (Cd, Co, Cr, Cu, Fe, Hg, Ni, Pb and Zn), and their effects on selected parameters of the AOS, including the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), phase II biotransformation enzyme glutathione-S-transferase (GST), the total glutathione (GSH) contents and sulfhydryl (SH) group concentrations, as well as cholinesterases (ChEs) activities in the liver, skin and muscle of P. kl. esculentus. Fro...g samples were collected at two sites (the Danube-Tisza-Danube canal (DTDC) and the river Ponjavica) in Serbia, which are characterized by different levels of metal pollution. Differences between the metal contents in different tissues showed that the skin of frogs from the DTDC accumulated statistically higher concentrations of Cd, Cu, Pb and Zn, while only the Fe concentration was lower. No significant differences between metal concentrations in muscle tissues of frogs from the DTDC and Ponjavica were observed. Examination of the parameters of the AOS revealed that frogs from the DTDC had higher concentrations of GSH in the liver and of SH groups in the skin and muscle, whereas the activities of the antioxidative enzymes SOD, GHS-Px and GR in the liver and of GR in the skin were lower than in frogs from the Ponjavica. The relationship between metal concentrations and AOS parameters showed the highest number of correlations with GSH, GR and CAT, and with Ni, Zn, Hg, Cr and Cd. Based on the results in this study, we concluded that increased concentrations of heavy metals in frog tissues can alter the AOS, which leads to higher concentrations of GSH and SH groups and lower activities of antioxidative enzymes. The response of the AOS to metal pollutants allowed us to make a distinction between different frog tissues, and to conclude that the liver and skin are more suitable for assessing metal-induced oxidative stress in frogs than muscle. (C) 2016 Elsevier Inc. All rights reserved.
Keywords:
Antioxidative defense system / Frogs tissues / Heavy metal ions / Oxidative stress / Pelophylax kl. esculentusSource:
Ecotoxicology and Environmental Safety, 2016, 128, 21-29Publisher:
- Academic Press Inc Elsevier Science, San Diego
Funding / projects:
- Molecular and physiological biomonitoring of aerobic organisms based on the determination of biochemical biomarkers of oxidative stress (RS-MESTD-Basic Research (BR or ON)-173041)
DOI: 10.1016/j.ecoenv.2016.02.005
ISSN: 0147-6513
PubMed: 26874985
WoS: 000373660700003
Scopus: 2-s2.0-84960944328
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Prokić, Marko D. AU - Borković-Mitić, Slavica S. AU - Krizmanić, Imre I. AU - Mutić, Jelena AU - Vukojević, Vesna AU - Nasia, Mohammed AU - Gavrić, Jelena P. AU - Despotović, Svetlana G. AU - Gavrilović, Branka R. AU - Radovanović, Tijana B. AU - Pavlović, Slađan Z. AU - Saičić, Zorica S. PY - 2016 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1909 AB - Heavy metal pollution of the aquatic environment is of great concern worldwide. Heavy metals are capable of inducing oxidative stress by increasing the formation of reactive oxygen species (ROS), and directly affecting the antioxidant defense system (AOS) in living organisms. The frog Pelophylax kl. esculentus is a semiaquatic species with semipermeable skin and a complex lifecycle, and represents a potentially useful bioindicator organism. The aim of this study was to investigate the accumulation of several heavy metals (Cd, Co, Cr, Cu, Fe, Hg, Ni, Pb and Zn), and their effects on selected parameters of the AOS, including the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), phase II biotransformation enzyme glutathione-S-transferase (GST), the total glutathione (GSH) contents and sulfhydryl (SH) group concentrations, as well as cholinesterases (ChEs) activities in the liver, skin and muscle of P. kl. esculentus. Frog samples were collected at two sites (the Danube-Tisza-Danube canal (DTDC) and the river Ponjavica) in Serbia, which are characterized by different levels of metal pollution. Differences between the metal contents in different tissues showed that the skin of frogs from the DTDC accumulated statistically higher concentrations of Cd, Cu, Pb and Zn, while only the Fe concentration was lower. No significant differences between metal concentrations in muscle tissues of frogs from the DTDC and Ponjavica were observed. Examination of the parameters of the AOS revealed that frogs from the DTDC had higher concentrations of GSH in the liver and of SH groups in the skin and muscle, whereas the activities of the antioxidative enzymes SOD, GHS-Px and GR in the liver and of GR in the skin were lower than in frogs from the Ponjavica. The relationship between metal concentrations and AOS parameters showed the highest number of correlations with GSH, GR and CAT, and with Ni, Zn, Hg, Cr and Cd. Based on the results in this study, we concluded that increased concentrations of heavy metals in frog tissues can alter the AOS, which leads to higher concentrations of GSH and SH groups and lower activities of antioxidative enzymes. The response of the AOS to metal pollutants allowed us to make a distinction between different frog tissues, and to conclude that the liver and skin are more suitable for assessing metal-induced oxidative stress in frogs than muscle. (C) 2016 Elsevier Inc. All rights reserved. PB - Academic Press Inc Elsevier Science, San Diego T2 - Ecotoxicology and Environmental Safety T1 - Antioxidative responses of the tissues of two wild populations of Pelophylax kl. esculentus frogs to heavy metal pollution VL - 128 SP - 21 EP - 29 DO - 10.1016/j.ecoenv.2016.02.005 ER -
@article{ author = "Prokić, Marko D. and Borković-Mitić, Slavica S. and Krizmanić, Imre I. and Mutić, Jelena and Vukojević, Vesna and Nasia, Mohammed and Gavrić, Jelena P. and Despotović, Svetlana G. and Gavrilović, Branka R. and Radovanović, Tijana B. and Pavlović, Slađan Z. and Saičić, Zorica S.", year = "2016", abstract = "Heavy metal pollution of the aquatic environment is of great concern worldwide. Heavy metals are capable of inducing oxidative stress by increasing the formation of reactive oxygen species (ROS), and directly affecting the antioxidant defense system (AOS) in living organisms. The frog Pelophylax kl. esculentus is a semiaquatic species with semipermeable skin and a complex lifecycle, and represents a potentially useful bioindicator organism. The aim of this study was to investigate the accumulation of several heavy metals (Cd, Co, Cr, Cu, Fe, Hg, Ni, Pb and Zn), and their effects on selected parameters of the AOS, including the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), phase II biotransformation enzyme glutathione-S-transferase (GST), the total glutathione (GSH) contents and sulfhydryl (SH) group concentrations, as well as cholinesterases (ChEs) activities in the liver, skin and muscle of P. kl. esculentus. Frog samples were collected at two sites (the Danube-Tisza-Danube canal (DTDC) and the river Ponjavica) in Serbia, which are characterized by different levels of metal pollution. Differences between the metal contents in different tissues showed that the skin of frogs from the DTDC accumulated statistically higher concentrations of Cd, Cu, Pb and Zn, while only the Fe concentration was lower. No significant differences between metal concentrations in muscle tissues of frogs from the DTDC and Ponjavica were observed. Examination of the parameters of the AOS revealed that frogs from the DTDC had higher concentrations of GSH in the liver and of SH groups in the skin and muscle, whereas the activities of the antioxidative enzymes SOD, GHS-Px and GR in the liver and of GR in the skin were lower than in frogs from the Ponjavica. The relationship between metal concentrations and AOS parameters showed the highest number of correlations with GSH, GR and CAT, and with Ni, Zn, Hg, Cr and Cd. Based on the results in this study, we concluded that increased concentrations of heavy metals in frog tissues can alter the AOS, which leads to higher concentrations of GSH and SH groups and lower activities of antioxidative enzymes. The response of the AOS to metal pollutants allowed us to make a distinction between different frog tissues, and to conclude that the liver and skin are more suitable for assessing metal-induced oxidative stress in frogs than muscle. (C) 2016 Elsevier Inc. All rights reserved.", publisher = "Academic Press Inc Elsevier Science, San Diego", journal = "Ecotoxicology and Environmental Safety", title = "Antioxidative responses of the tissues of two wild populations of Pelophylax kl. esculentus frogs to heavy metal pollution", volume = "128", pages = "21-29", doi = "10.1016/j.ecoenv.2016.02.005" }
Prokić, M. D., Borković-Mitić, S. S., Krizmanić, I. I., Mutić, J., Vukojević, V., Nasia, M., Gavrić, J. P., Despotović, S. G., Gavrilović, B. R., Radovanović, T. B., Pavlović, S. Z.,& Saičić, Z. S.. (2016). Antioxidative responses of the tissues of two wild populations of Pelophylax kl. esculentus frogs to heavy metal pollution. in Ecotoxicology and Environmental Safety Academic Press Inc Elsevier Science, San Diego., 128, 21-29. https://doi.org/10.1016/j.ecoenv.2016.02.005
Prokić MD, Borković-Mitić SS, Krizmanić II, Mutić J, Vukojević V, Nasia M, Gavrić JP, Despotović SG, Gavrilović BR, Radovanović TB, Pavlović SZ, Saičić ZS. Antioxidative responses of the tissues of two wild populations of Pelophylax kl. esculentus frogs to heavy metal pollution. in Ecotoxicology and Environmental Safety. 2016;128:21-29. doi:10.1016/j.ecoenv.2016.02.005 .
Prokić, Marko D., Borković-Mitić, Slavica S., Krizmanić, Imre I., Mutić, Jelena, Vukojević, Vesna, Nasia, Mohammed, Gavrić, Jelena P., Despotović, Svetlana G., Gavrilović, Branka R., Radovanović, Tijana B., Pavlović, Slađan Z., Saičić, Zorica S., "Antioxidative responses of the tissues of two wild populations of Pelophylax kl. esculentus frogs to heavy metal pollution" in Ecotoxicology and Environmental Safety, 128 (2016):21-29, https://doi.org/10.1016/j.ecoenv.2016.02.005 . .