Antioxidant Capacity Determination of Complex Samples and Individual Phenolics - Multilateral Approach
Samo za registrovane korisnike
2016
Autori
Petrović, Marija S.Sužnjević, Desanka
Pastor, Ferenc
Veljović, Mile S.
Pezo, Lato
Antić, Mališa
Gorjanović, Stanislava
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Antioxidant (AO) capacity of various medicinal plants extracts and phenolic compounds was assessed by the most widely used spectrophotometric assays such as ferric reducing antioxidant power (FRAP) and scavenging of 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). In addition, two direct current (DC) polarographic assays, one based on a decrease of anodic current of [Hg(O2H)(OH)]-HydroxoPerhydroxoMercury(II) Complex (HPMC) formation in alkaline solution of H2O2, at the potential of mercury dissolution and another recently developed Mercury Reduction Antioxidant Power (MRAP), based on a a decrease of cathodic current of Hg(II) reduction were employed. Percentage of both currents decrease was plotted versus the volume of gradually added complex samples or the amount of individual ones and the slopes of these plots were used to express AO capacity. Total phenolic content (TPC) of extracts was determined by Folin-Ciocalteu (FC) assay. Co...rrelations between applied assays were calculated by regression analysis. Relative Antioxidant Capacity Index (RACI), calculated by assigning equal weight to all applied assays and Phenolic Antioxidant Coefficients (PAC), calculated as a ratio between particular AO capacity and TPC, were used to achieve more comprehensive comparison between analyzed samples, as well as applied assays.
Ključne reči:
Antioxidants / hydrogen peroxide / mercury reduction / polarographyIzvor:
Combinatorial Chemistry and High Throughput Screening, 2016, 19, 1, 58-65Izdavač:
- Bentham Science Publ Ltd, Sharjah
Finansiranje / projekti:
- Uticaj sorte i uslova gajenja na sadržaj bioaktivnih komponenti jagodastog i koštičavog voća i dobijanje biološki vrednih proizvoda poboljšanim i novim tehnologijama (RS-MESTD-Technological Development (TD or TR)-31093)
- Modifikacije antioksidativnog metabolizma biljaka sa ciljem povećanja tolerancije na abiotski stres i identifikacija novih biomarkera sa primenom u remedijaciji i monitoringu degradiranih staništa (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43010)
- Osmotska dehidratacija hrane - energetski i ekološki aspekti održive proizvodnje (RS-MESTD-Technological Development (TD or TR)-31055)
DOI: 10.2174/1386207318666151102094227
ISSN: 1386-2073
PubMed: 26522992
WoS: 000368910000007
Scopus: 2-s2.0-84959260890
Kolekcije
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Petrović, Marija S. AU - Sužnjević, Desanka AU - Pastor, Ferenc AU - Veljović, Mile S. AU - Pezo, Lato AU - Antić, Mališa AU - Gorjanović, Stanislava PY - 2016 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1963 AB - Antioxidant (AO) capacity of various medicinal plants extracts and phenolic compounds was assessed by the most widely used spectrophotometric assays such as ferric reducing antioxidant power (FRAP) and scavenging of 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). In addition, two direct current (DC) polarographic assays, one based on a decrease of anodic current of [Hg(O2H)(OH)]-HydroxoPerhydroxoMercury(II) Complex (HPMC) formation in alkaline solution of H2O2, at the potential of mercury dissolution and another recently developed Mercury Reduction Antioxidant Power (MRAP), based on a a decrease of cathodic current of Hg(II) reduction were employed. Percentage of both currents decrease was plotted versus the volume of gradually added complex samples or the amount of individual ones and the slopes of these plots were used to express AO capacity. Total phenolic content (TPC) of extracts was determined by Folin-Ciocalteu (FC) assay. Correlations between applied assays were calculated by regression analysis. Relative Antioxidant Capacity Index (RACI), calculated by assigning equal weight to all applied assays and Phenolic Antioxidant Coefficients (PAC), calculated as a ratio between particular AO capacity and TPC, were used to achieve more comprehensive comparison between analyzed samples, as well as applied assays. PB - Bentham Science Publ Ltd, Sharjah T2 - Combinatorial Chemistry and High Throughput Screening T1 - Antioxidant Capacity Determination of Complex Samples and Individual Phenolics - Multilateral Approach VL - 19 IS - 1 SP - 58 EP - 65 DO - 10.2174/1386207318666151102094227 ER -
@article{ author = "Petrović, Marija S. and Sužnjević, Desanka and Pastor, Ferenc and Veljović, Mile S. and Pezo, Lato and Antić, Mališa and Gorjanović, Stanislava", year = "2016", abstract = "Antioxidant (AO) capacity of various medicinal plants extracts and phenolic compounds was assessed by the most widely used spectrophotometric assays such as ferric reducing antioxidant power (FRAP) and scavenging of 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). In addition, two direct current (DC) polarographic assays, one based on a decrease of anodic current of [Hg(O2H)(OH)]-HydroxoPerhydroxoMercury(II) Complex (HPMC) formation in alkaline solution of H2O2, at the potential of mercury dissolution and another recently developed Mercury Reduction Antioxidant Power (MRAP), based on a a decrease of cathodic current of Hg(II) reduction were employed. Percentage of both currents decrease was plotted versus the volume of gradually added complex samples or the amount of individual ones and the slopes of these plots were used to express AO capacity. Total phenolic content (TPC) of extracts was determined by Folin-Ciocalteu (FC) assay. Correlations between applied assays were calculated by regression analysis. Relative Antioxidant Capacity Index (RACI), calculated by assigning equal weight to all applied assays and Phenolic Antioxidant Coefficients (PAC), calculated as a ratio between particular AO capacity and TPC, were used to achieve more comprehensive comparison between analyzed samples, as well as applied assays.", publisher = "Bentham Science Publ Ltd, Sharjah", journal = "Combinatorial Chemistry and High Throughput Screening", title = "Antioxidant Capacity Determination of Complex Samples and Individual Phenolics - Multilateral Approach", volume = "19", number = "1", pages = "58-65", doi = "10.2174/1386207318666151102094227" }
Petrović, M. S., Sužnjević, D., Pastor, F., Veljović, M. S., Pezo, L., Antić, M.,& Gorjanović, S.. (2016). Antioxidant Capacity Determination of Complex Samples and Individual Phenolics - Multilateral Approach. in Combinatorial Chemistry and High Throughput Screening Bentham Science Publ Ltd, Sharjah., 19(1), 58-65. https://doi.org/10.2174/1386207318666151102094227
Petrović MS, Sužnjević D, Pastor F, Veljović MS, Pezo L, Antić M, Gorjanović S. Antioxidant Capacity Determination of Complex Samples and Individual Phenolics - Multilateral Approach. in Combinatorial Chemistry and High Throughput Screening. 2016;19(1):58-65. doi:10.2174/1386207318666151102094227 .
Petrović, Marija S., Sužnjević, Desanka, Pastor, Ferenc, Veljović, Mile S., Pezo, Lato, Antić, Mališa, Gorjanović, Stanislava, "Antioxidant Capacity Determination of Complex Samples and Individual Phenolics - Multilateral Approach" in Combinatorial Chemistry and High Throughput Screening, 19, no. 1 (2016):58-65, https://doi.org/10.2174/1386207318666151102094227 . .