Wild Thymus capitatus Hoff. Et Link. Chemical Composition, Antioxidant and Antimicrobial Activities of the Essential Oil
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2018
Authors
Marin, MarijaNovaković, Miroslav M.
Vučković, Ivan M.
Tešević, Vele
Kolarević, Stoimir
Vuković-Gačić, Branka
Article (Published version)
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The composition of the essential oil (EO) obtained by hydrodistillation from the leaves of Thymus capitatus Hoff. et Link. growing wild in Sythonia (Greece) was analysed by GC-FID, GC-MS and quantitative H-1 NMR spectroscopy. Thirty nine compounds were identified, representing approximately 99.0 % of the oil. Major component determined by GC-FID was carvacrol (81.8 %), followed by linalool (3.5 %) and (E)-caryophyllenc (3.5 %). Abundance of carvacrol determined by quantitative H-1 NMR spectroscopy was 74.9 % and was in reasonable agreement with GC-FID result. The oil was tested for the antioxidant activity in DPPH assay and showed a dose dependent free radical scavenging activity with EC50 value of 1.03 mu g/ml. Antimicrobial effect of the EO was tested against Gram negative, Gram positive bacteria, and fungi. The tested EO showed significant activity against fungi, especially S. cerevisiae. The most sensitive bacteria were E. coil ATCC 25922, E. fecalis and S. epidermidis. Human patho...gen Burkholderia cepacia, ATCC 25416 was used in the investigation of T capitatus for the fast time.
Keywords:
Thymus capitatus / essential oil / GC-FID / GC-MS / antioxidant / antimicrobialSource:
Journal of Essential Oil Bearing Plants, 2018, 21, 2, 388-399Publisher:
- Taylor & Francis Ltd, Abingdon
Funding / projects:
- Natural products of wild, cultivated and edible plants: structure and bioactivity determination (RS-MESTD-Basic Research (BR or ON)-172053)
- Bioactive natural products as potential sources of new pharmaceuticals and food supplements (RS-MESTD-Basic Research (BR or ON)-172058)
DOI: 10.1080/0972060X.2018.1449668
ISSN: 0972-060X
WoS: 000432900400008
Scopus: 2-s2.0-85046820016
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Marin, Marija AU - Novaković, Miroslav M. AU - Vučković, Ivan M. AU - Tešević, Vele AU - Kolarević, Stoimir AU - Vuković-Gačić, Branka PY - 2018 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2143 AB - The composition of the essential oil (EO) obtained by hydrodistillation from the leaves of Thymus capitatus Hoff. et Link. growing wild in Sythonia (Greece) was analysed by GC-FID, GC-MS and quantitative H-1 NMR spectroscopy. Thirty nine compounds were identified, representing approximately 99.0 % of the oil. Major component determined by GC-FID was carvacrol (81.8 %), followed by linalool (3.5 %) and (E)-caryophyllenc (3.5 %). Abundance of carvacrol determined by quantitative H-1 NMR spectroscopy was 74.9 % and was in reasonable agreement with GC-FID result. The oil was tested for the antioxidant activity in DPPH assay and showed a dose dependent free radical scavenging activity with EC50 value of 1.03 mu g/ml. Antimicrobial effect of the EO was tested against Gram negative, Gram positive bacteria, and fungi. The tested EO showed significant activity against fungi, especially S. cerevisiae. The most sensitive bacteria were E. coil ATCC 25922, E. fecalis and S. epidermidis. Human pathogen Burkholderia cepacia, ATCC 25416 was used in the investigation of T capitatus for the fast time. PB - Taylor & Francis Ltd, Abingdon T2 - Journal of Essential Oil Bearing Plants T1 - Wild Thymus capitatus Hoff. Et Link. Chemical Composition, Antioxidant and Antimicrobial Activities of the Essential Oil VL - 21 IS - 2 SP - 388 EP - 399 DO - 10.1080/0972060X.2018.1449668 ER -
@article{ author = "Marin, Marija and Novaković, Miroslav M. and Vučković, Ivan M. and Tešević, Vele and Kolarević, Stoimir and Vuković-Gačić, Branka", year = "2018", abstract = "The composition of the essential oil (EO) obtained by hydrodistillation from the leaves of Thymus capitatus Hoff. et Link. growing wild in Sythonia (Greece) was analysed by GC-FID, GC-MS and quantitative H-1 NMR spectroscopy. Thirty nine compounds were identified, representing approximately 99.0 % of the oil. Major component determined by GC-FID was carvacrol (81.8 %), followed by linalool (3.5 %) and (E)-caryophyllenc (3.5 %). Abundance of carvacrol determined by quantitative H-1 NMR spectroscopy was 74.9 % and was in reasonable agreement with GC-FID result. The oil was tested for the antioxidant activity in DPPH assay and showed a dose dependent free radical scavenging activity with EC50 value of 1.03 mu g/ml. Antimicrobial effect of the EO was tested against Gram negative, Gram positive bacteria, and fungi. The tested EO showed significant activity against fungi, especially S. cerevisiae. The most sensitive bacteria were E. coil ATCC 25922, E. fecalis and S. epidermidis. Human pathogen Burkholderia cepacia, ATCC 25416 was used in the investigation of T capitatus for the fast time.", publisher = "Taylor & Francis Ltd, Abingdon", journal = "Journal of Essential Oil Bearing Plants", title = "Wild Thymus capitatus Hoff. Et Link. Chemical Composition, Antioxidant and Antimicrobial Activities of the Essential Oil", volume = "21", number = "2", pages = "388-399", doi = "10.1080/0972060X.2018.1449668" }
Marin, M., Novaković, M. M., Vučković, I. M., Tešević, V., Kolarević, S.,& Vuković-Gačić, B.. (2018). Wild Thymus capitatus Hoff. Et Link. Chemical Composition, Antioxidant and Antimicrobial Activities of the Essential Oil. in Journal of Essential Oil Bearing Plants Taylor & Francis Ltd, Abingdon., 21(2), 388-399. https://doi.org/10.1080/0972060X.2018.1449668
Marin M, Novaković MM, Vučković IM, Tešević V, Kolarević S, Vuković-Gačić B. Wild Thymus capitatus Hoff. Et Link. Chemical Composition, Antioxidant and Antimicrobial Activities of the Essential Oil. in Journal of Essential Oil Bearing Plants. 2018;21(2):388-399. doi:10.1080/0972060X.2018.1449668 .
Marin, Marija, Novaković, Miroslav M., Vučković, Ivan M., Tešević, Vele, Kolarević, Stoimir, Vuković-Gačić, Branka, "Wild Thymus capitatus Hoff. Et Link. Chemical Composition, Antioxidant and Antimicrobial Activities of the Essential Oil" in Journal of Essential Oil Bearing Plants, 21, no. 2 (2018):388-399, https://doi.org/10.1080/0972060X.2018.1449668 . .