Analytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solvents
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2018
Authors
Vukojević, VesnaĐurđić, Slađana Z.
Šovrc, Ľubomír
Ćirković-Veličković, Tanja
Mutić, Jelena
Stanković, Dalibor
Article (Published version)
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Show full item recordAbstract
Ergosterol (Erg) is the major sterol component in the plasma membranes of fungi. The main goal of this work was to develop rapid, reliable, and cost-effective method toward Erg detection based on application of unmodified boron-doped diamond electrode. Several organic solvents were tested (acetonitrile, dimethyformamide, dimethylsulfoxide, dichloromethane), and it was found that best analytical characteristics were achieved using acetonitrile containing 0.1 M tetrabutylammonium hexa-fluorophosphate as supporting electrolyte. Oval shaped and well-defined oxidation peak was recorded at potential of around 1.17 V versus Ag/Ag+ non-aqueous reference electrode. Under optimized experimental conditions, linear working range from 2 to 200 mu M was estimated, with limit of detection of 0.7 mu M, using square wave voltammetry. Negligible effect of possible interfering compound was observed. Proposed methodology was successfully applied for estimation of Erg content in mushrooms extract with sati...sfactory results in comparison with spectrophotometric procedure. Obtained results clearly show that developed analytical methodology can be adequate replacement for the, up to date, used methods and open-novel approach for ergosterol detection.
Keywords:
Ergosterol / Boron-doped diamond electrode / MushroomsSource:
Food Analytical Methods, 2018, 11, 9, 2590-2596Publisher:
- Springer, New York
Funding / projects:
- Application of advanced oxidation processes and nanostructured oxide materials for the removal of pollutants from the environment, development and optimisation of instrumental techniques for efficiency monitoring (RS-MESTD-Basic Research (BR or ON)-172030)
- Strengthening of the MagBioVin Research and Innovation Team for Development of Novel Approaches for Tumour Therapy based on Nanostructured Materials (EU-FP7-621375)
- Grant Agency of the Slovak Republic [1/0489/16]
DOI: 10.1007/s12161-018-1249-3
ISSN: 1936-9751
WoS: 000438995800026
Scopus: 2-s2.0-85045073042
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Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Vukojević, Vesna AU - Đurđić, Slađana Z. AU - Šovrc, Ľubomír AU - Ćirković-Veličković, Tanja AU - Mutić, Jelena AU - Stanković, Dalibor PY - 2018 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2184 AB - Ergosterol (Erg) is the major sterol component in the plasma membranes of fungi. The main goal of this work was to develop rapid, reliable, and cost-effective method toward Erg detection based on application of unmodified boron-doped diamond electrode. Several organic solvents were tested (acetonitrile, dimethyformamide, dimethylsulfoxide, dichloromethane), and it was found that best analytical characteristics were achieved using acetonitrile containing 0.1 M tetrabutylammonium hexa-fluorophosphate as supporting electrolyte. Oval shaped and well-defined oxidation peak was recorded at potential of around 1.17 V versus Ag/Ag+ non-aqueous reference electrode. Under optimized experimental conditions, linear working range from 2 to 200 mu M was estimated, with limit of detection of 0.7 mu M, using square wave voltammetry. Negligible effect of possible interfering compound was observed. Proposed methodology was successfully applied for estimation of Erg content in mushrooms extract with satisfactory results in comparison with spectrophotometric procedure. Obtained results clearly show that developed analytical methodology can be adequate replacement for the, up to date, used methods and open-novel approach for ergosterol detection. PB - Springer, New York T2 - Food Analytical Methods T1 - Analytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solvents VL - 11 IS - 9 SP - 2590 EP - 2596 DO - 10.1007/s12161-018-1249-3 ER -
@article{ author = "Vukojević, Vesna and Đurđić, Slađana Z. and Šovrc, Ľubomír and Ćirković-Veličković, Tanja and Mutić, Jelena and Stanković, Dalibor", year = "2018", abstract = "Ergosterol (Erg) is the major sterol component in the plasma membranes of fungi. The main goal of this work was to develop rapid, reliable, and cost-effective method toward Erg detection based on application of unmodified boron-doped diamond electrode. Several organic solvents were tested (acetonitrile, dimethyformamide, dimethylsulfoxide, dichloromethane), and it was found that best analytical characteristics were achieved using acetonitrile containing 0.1 M tetrabutylammonium hexa-fluorophosphate as supporting electrolyte. Oval shaped and well-defined oxidation peak was recorded at potential of around 1.17 V versus Ag/Ag+ non-aqueous reference electrode. Under optimized experimental conditions, linear working range from 2 to 200 mu M was estimated, with limit of detection of 0.7 mu M, using square wave voltammetry. Negligible effect of possible interfering compound was observed. Proposed methodology was successfully applied for estimation of Erg content in mushrooms extract with satisfactory results in comparison with spectrophotometric procedure. Obtained results clearly show that developed analytical methodology can be adequate replacement for the, up to date, used methods and open-novel approach for ergosterol detection.", publisher = "Springer, New York", journal = "Food Analytical Methods", title = "Analytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solvents", volume = "11", number = "9", pages = "2590-2596", doi = "10.1007/s12161-018-1249-3" }
Vukojević, V., Đurđić, S. Z., Šovrc, Ľ., Ćirković-Veličković, T., Mutić, J.,& Stanković, D.. (2018). Analytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solvents. in Food Analytical Methods Springer, New York., 11(9), 2590-2596. https://doi.org/10.1007/s12161-018-1249-3
Vukojević V, Đurđić SZ, Šovrc Ľ, Ćirković-Veličković T, Mutić J, Stanković D. Analytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solvents. in Food Analytical Methods. 2018;11(9):2590-2596. doi:10.1007/s12161-018-1249-3 .
Vukojević, Vesna, Đurđić, Slađana Z., Šovrc, Ľubomír, Ćirković-Veličković, Tanja, Mutić, Jelena, Stanković, Dalibor, "Analytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solvents" in Food Analytical Methods, 11, no. 9 (2018):2590-2596, https://doi.org/10.1007/s12161-018-1249-3 . .