Millipedes vs. pathogens: Defensive secretions of some julids (Diplopoda: Julida) as potential antimicrobial agents
Authorized Users Only
2018
Authors
Ilic, BojanDimkić, Ivica
Unković, Nikola
Ljaljević-Grbić, Milica
Vukojević, Jelena
Vujisić, Ljubodrag V.
Tešević, Vele
Stanković, Slaviša
Makarov, Slobodan E.
Lučić, Luka
Article (Published version)
Metadata
Show full item recordAbstract
In the light of growing interest in discovering new sources of natural antimicrobial agents, we extracted and tested the efficiency of julid defensive secretions in comparison with commercially used antibiotic and antimycotics. This work involved chemical characterization of the defensive secretions of Cylindroiulus boleti (C. L. Koch, 1847), Megaphyllum bosniense (Verhoeff, 1897) and M.unilineatum (C. L. Koch, 1838), as well as in vitro evaluation of their antimicrobial activity against 11 bacteria, one yeast and eight filamentous fungi. Compounds of the analysed defensive secretions included p-benzoquinones, alkyl esters of fatty acids and ketones. Ketones were recorded for the first time in the order Julida, and they were detected in secretions of both Megaphyllum species. All three analysed defensive secretions showed antibacterial and antifungal potential against all of the tested pathogens. Staphylococcus aureus proved to be the most sensitive bacterial strain to all analysed sec...retions. Also, defensive secretion of M.unilineatum showed significant antibacterial potential against Bacillus subtilis. The most resistant bacterial strains in this study were Escherichia coli, Erwinia persicina and Pseudomonas syringae pv. tomato. Analysed defensive secretions achieved the strongest antifungal activity against Aspergillus parasiticus (secretions of all three millipede species), Penicillium griseofulvum (secretions of C.boleti and M.bosniense) and Cladosporium oxysporum (secretion of M.unilineatum). The most resistant mycromycetes were A.niger (to all tested defensive secretions), A.flavus (to secretion of M.unilineatum) and P.lanosum (to secretions of C.boleti and M.bosniense). Our results showed a generally lower level of activity compared to antibiotic and a significantly higher level compared to antimycotics. The results of this study elucidate and open opportunities for further research in the field of millipede chemical ecology.
Keywords:
antibacterial and antifungal activity / diplopods / gas chromatography-mass spectrometry / microdilution / pathogens / quinonesSource:
Journal of Applied Entomology, 2018, 142, 8, 775-791Publisher:
- Wiley, Hoboken
Funding / projects:
- Natural products of wild, cultivated and edible plants: structure and bioactivity determination (RS-MESTD-Basic Research (BR or ON)-172053)
- Molecular characterization of bacteria from genera Bacillus and Pseudomonas as potential agents for biological control (RS-MESTD-Basic Research (BR or ON)-173026)
- Characterization and application of fungal metabolites and assessment of new biofungicides potential (RS-MESTD-Basic Research (BR or ON)-173032)
- Ontogenetic characterization of phylogenetic biodiversity (RS-MESTD-Basic Research (BR or ON)-173038)
Note:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3238
DOI: 10.1111/jen.12526
ISSN: 0931-2048
WoS: 000441246600006
Scopus: 2-s2.0-85050940029
Collections
Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Ilic, Bojan AU - Dimkić, Ivica AU - Unković, Nikola AU - Ljaljević-Grbić, Milica AU - Vukojević, Jelena AU - Vujisić, Ljubodrag V. AU - Tešević, Vele AU - Stanković, Slaviša AU - Makarov, Slobodan E. AU - Lučić, Luka PY - 2018 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2198 AB - In the light of growing interest in discovering new sources of natural antimicrobial agents, we extracted and tested the efficiency of julid defensive secretions in comparison with commercially used antibiotic and antimycotics. This work involved chemical characterization of the defensive secretions of Cylindroiulus boleti (C. L. Koch, 1847), Megaphyllum bosniense (Verhoeff, 1897) and M.unilineatum (C. L. Koch, 1838), as well as in vitro evaluation of their antimicrobial activity against 11 bacteria, one yeast and eight filamentous fungi. Compounds of the analysed defensive secretions included p-benzoquinones, alkyl esters of fatty acids and ketones. Ketones were recorded for the first time in the order Julida, and they were detected in secretions of both Megaphyllum species. All three analysed defensive secretions showed antibacterial and antifungal potential against all of the tested pathogens. Staphylococcus aureus proved to be the most sensitive bacterial strain to all analysed secretions. Also, defensive secretion of M.unilineatum showed significant antibacterial potential against Bacillus subtilis. The most resistant bacterial strains in this study were Escherichia coli, Erwinia persicina and Pseudomonas syringae pv. tomato. Analysed defensive secretions achieved the strongest antifungal activity against Aspergillus parasiticus (secretions of all three millipede species), Penicillium griseofulvum (secretions of C.boleti and M.bosniense) and Cladosporium oxysporum (secretion of M.unilineatum). The most resistant mycromycetes were A.niger (to all tested defensive secretions), A.flavus (to secretion of M.unilineatum) and P.lanosum (to secretions of C.boleti and M.bosniense). Our results showed a generally lower level of activity compared to antibiotic and a significantly higher level compared to antimycotics. The results of this study elucidate and open opportunities for further research in the field of millipede chemical ecology. PB - Wiley, Hoboken T2 - Journal of Applied Entomology T1 - Millipedes vs. pathogens: Defensive secretions of some julids (Diplopoda: Julida) as potential antimicrobial agents VL - 142 IS - 8 SP - 775 EP - 791 DO - 10.1111/jen.12526 ER -
@article{ author = "Ilic, Bojan and Dimkić, Ivica and Unković, Nikola and Ljaljević-Grbić, Milica and Vukojević, Jelena and Vujisić, Ljubodrag V. and Tešević, Vele and Stanković, Slaviša and Makarov, Slobodan E. and Lučić, Luka", year = "2018", abstract = "In the light of growing interest in discovering new sources of natural antimicrobial agents, we extracted and tested the efficiency of julid defensive secretions in comparison with commercially used antibiotic and antimycotics. This work involved chemical characterization of the defensive secretions of Cylindroiulus boleti (C. L. Koch, 1847), Megaphyllum bosniense (Verhoeff, 1897) and M.unilineatum (C. L. Koch, 1838), as well as in vitro evaluation of their antimicrobial activity against 11 bacteria, one yeast and eight filamentous fungi. Compounds of the analysed defensive secretions included p-benzoquinones, alkyl esters of fatty acids and ketones. Ketones were recorded for the first time in the order Julida, and they were detected in secretions of both Megaphyllum species. All three analysed defensive secretions showed antibacterial and antifungal potential against all of the tested pathogens. Staphylococcus aureus proved to be the most sensitive bacterial strain to all analysed secretions. Also, defensive secretion of M.unilineatum showed significant antibacterial potential against Bacillus subtilis. The most resistant bacterial strains in this study were Escherichia coli, Erwinia persicina and Pseudomonas syringae pv. tomato. Analysed defensive secretions achieved the strongest antifungal activity against Aspergillus parasiticus (secretions of all three millipede species), Penicillium griseofulvum (secretions of C.boleti and M.bosniense) and Cladosporium oxysporum (secretion of M.unilineatum). The most resistant mycromycetes were A.niger (to all tested defensive secretions), A.flavus (to secretion of M.unilineatum) and P.lanosum (to secretions of C.boleti and M.bosniense). Our results showed a generally lower level of activity compared to antibiotic and a significantly higher level compared to antimycotics. The results of this study elucidate and open opportunities for further research in the field of millipede chemical ecology.", publisher = "Wiley, Hoboken", journal = "Journal of Applied Entomology", title = "Millipedes vs. pathogens: Defensive secretions of some julids (Diplopoda: Julida) as potential antimicrobial agents", volume = "142", number = "8", pages = "775-791", doi = "10.1111/jen.12526" }
Ilic, B., Dimkić, I., Unković, N., Ljaljević-Grbić, M., Vukojević, J., Vujisić, L. V., Tešević, V., Stanković, S., Makarov, S. E.,& Lučić, L.. (2018). Millipedes vs. pathogens: Defensive secretions of some julids (Diplopoda: Julida) as potential antimicrobial agents. in Journal of Applied Entomology Wiley, Hoboken., 142(8), 775-791. https://doi.org/10.1111/jen.12526
Ilic B, Dimkić I, Unković N, Ljaljević-Grbić M, Vukojević J, Vujisić LV, Tešević V, Stanković S, Makarov SE, Lučić L. Millipedes vs. pathogens: Defensive secretions of some julids (Diplopoda: Julida) as potential antimicrobial agents. in Journal of Applied Entomology. 2018;142(8):775-791. doi:10.1111/jen.12526 .
Ilic, Bojan, Dimkić, Ivica, Unković, Nikola, Ljaljević-Grbić, Milica, Vukojević, Jelena, Vujisić, Ljubodrag V., Tešević, Vele, Stanković, Slaviša, Makarov, Slobodan E., Lučić, Luka, "Millipedes vs. pathogens: Defensive secretions of some julids (Diplopoda: Julida) as potential antimicrobial agents" in Journal of Applied Entomology, 142, no. 8 (2018):775-791, https://doi.org/10.1111/jen.12526 . .