Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers
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2017
Authors
Banjanac, TijanaDragičević, Milan
Šiler, Branislav
Gašić, Uroš M.
Bohanec, Borut
Nestorović Živković, Jasmina
Trifunović, Snežana S.
Mišić, Danijela
Article (Published version)
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Species within the genus Centaurium readily hybridize and polyploid complexes are often seen in natural populations. We describe phytochemical profiles of newly discovered allohexaploid hybrid, here named Centaurium pannonicum, and its parental tetraploid species C. erythraea and rare C. littorale ssp. compressum. Our aim was to examine chemodiversity of these taxa in the area of Vojvodina (North Serbia) and to perform metabolomics search for chemical classifiers which would provide high resolution discrimination of parental and hybrid individuals. In sum, UHPLC-MS/MS Orbitrap metabolomics fingerprinting revealed seventy compounds in methanol extracts. Despite the lack of qualitative chemical novelty in hybrid plants, UHPLC-qqqMS targeted metabolomics approach, aimed at three secoiridoid compounds and seventeen phenolics, pointed to considerable differences in quantitative composition of these dominant compounds among the plant taxa studied. In addition to the difference in the ploidy ...levels, the hybrid taxon was well distinguished from both parental species based on metabolite profiles, and, for most individuals, positioned intermediately to the parental taxa in both PCA and hierarchical clustering. After optimizing and comparing several statistical learning methods, it was possible to narrow the number of taxonomic classifiers to five (three xanthones, one secoiridoid glycoside, and one phenolic acid), while increasing the differentiation resolution. The presented metabolomics approach will certainly, along with morphometrics and molecular genetics studies, have high impact on further elucidation of complex relationships among taxa within the genus Centaurium.
Keywords:
Centaurium Hill / Gentianaceae / Centaury / Interspecific hybridization / Polyploidy / Phytochemical profiles / Targeted metabolomics / Chemometrics / Secoiridoids / PhenolicsSource:
Phytochemistry, 2017, 140, 27-44Publisher:
- Pergamon-Elsevier Science Ltd, Oxford
Funding / projects:
- Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds (RS-173024)
- Structure-properties relationships of natural and synthetic molecules and their metal complexes (RS-172017)
Note:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3098
DOI: 10.1016/j.phytochem.2017.04.005
ISSN: 0031-9422
PubMed: 28448798
WoS: 000403993200003
Scopus: 2-s2.0-85018485899
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Banjanac, Tijana AU - Dragičević, Milan AU - Šiler, Branislav AU - Gašić, Uroš M. AU - Bohanec, Borut AU - Nestorović Živković, Jasmina AU - Trifunović, Snežana S. AU - Mišić, Danijela PY - 2017 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2477 AB - Species within the genus Centaurium readily hybridize and polyploid complexes are often seen in natural populations. We describe phytochemical profiles of newly discovered allohexaploid hybrid, here named Centaurium pannonicum, and its parental tetraploid species C. erythraea and rare C. littorale ssp. compressum. Our aim was to examine chemodiversity of these taxa in the area of Vojvodina (North Serbia) and to perform metabolomics search for chemical classifiers which would provide high resolution discrimination of parental and hybrid individuals. In sum, UHPLC-MS/MS Orbitrap metabolomics fingerprinting revealed seventy compounds in methanol extracts. Despite the lack of qualitative chemical novelty in hybrid plants, UHPLC-qqqMS targeted metabolomics approach, aimed at three secoiridoid compounds and seventeen phenolics, pointed to considerable differences in quantitative composition of these dominant compounds among the plant taxa studied. In addition to the difference in the ploidy levels, the hybrid taxon was well distinguished from both parental species based on metabolite profiles, and, for most individuals, positioned intermediately to the parental taxa in both PCA and hierarchical clustering. After optimizing and comparing several statistical learning methods, it was possible to narrow the number of taxonomic classifiers to five (three xanthones, one secoiridoid glycoside, and one phenolic acid), while increasing the differentiation resolution. The presented metabolomics approach will certainly, along with morphometrics and molecular genetics studies, have high impact on further elucidation of complex relationships among taxa within the genus Centaurium. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Phytochemistry T1 - Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers VL - 140 SP - 27 EP - 44 DO - 10.1016/j.phytochem.2017.04.005 ER -
@article{ author = "Banjanac, Tijana and Dragičević, Milan and Šiler, Branislav and Gašić, Uroš M. and Bohanec, Borut and Nestorović Živković, Jasmina and Trifunović, Snežana S. and Mišić, Danijela", year = "2017", abstract = "Species within the genus Centaurium readily hybridize and polyploid complexes are often seen in natural populations. We describe phytochemical profiles of newly discovered allohexaploid hybrid, here named Centaurium pannonicum, and its parental tetraploid species C. erythraea and rare C. littorale ssp. compressum. Our aim was to examine chemodiversity of these taxa in the area of Vojvodina (North Serbia) and to perform metabolomics search for chemical classifiers which would provide high resolution discrimination of parental and hybrid individuals. In sum, UHPLC-MS/MS Orbitrap metabolomics fingerprinting revealed seventy compounds in methanol extracts. Despite the lack of qualitative chemical novelty in hybrid plants, UHPLC-qqqMS targeted metabolomics approach, aimed at three secoiridoid compounds and seventeen phenolics, pointed to considerable differences in quantitative composition of these dominant compounds among the plant taxa studied. In addition to the difference in the ploidy levels, the hybrid taxon was well distinguished from both parental species based on metabolite profiles, and, for most individuals, positioned intermediately to the parental taxa in both PCA and hierarchical clustering. After optimizing and comparing several statistical learning methods, it was possible to narrow the number of taxonomic classifiers to five (three xanthones, one secoiridoid glycoside, and one phenolic acid), while increasing the differentiation resolution. The presented metabolomics approach will certainly, along with morphometrics and molecular genetics studies, have high impact on further elucidation of complex relationships among taxa within the genus Centaurium.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Phytochemistry", title = "Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers", volume = "140", pages = "27-44", doi = "10.1016/j.phytochem.2017.04.005" }
Banjanac, T., Dragičević, M., Šiler, B., Gašić, U. M., Bohanec, B., Nestorović Živković, J., Trifunović, S. S.,& Mišić, D.. (2017). Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers. in Phytochemistry Pergamon-Elsevier Science Ltd, Oxford., 140, 27-44. https://doi.org/10.1016/j.phytochem.2017.04.005
Banjanac T, Dragičević M, Šiler B, Gašić UM, Bohanec B, Nestorović Živković J, Trifunović SS, Mišić D. Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers. in Phytochemistry. 2017;140:27-44. doi:10.1016/j.phytochem.2017.04.005 .
Banjanac, Tijana, Dragičević, Milan, Šiler, Branislav, Gašić, Uroš M., Bohanec, Borut, Nestorović Živković, Jasmina, Trifunović, Snežana S., Mišić, Danijela, "Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers" in Phytochemistry, 140 (2017):27-44, https://doi.org/10.1016/j.phytochem.2017.04.005 . .