Advanced electrochemical platform for determination of cytostatic drug flutamide in various matrices using a boron-doped diamond electrode
Abstract
An innovative, rapid and simple electrochemical approach for the reliable quantification of cytostatic drug flutamide (FLU) in various matrices is herein proposed. This platform involves coupling of differential pulse (DPV) and square-wave voltammetry (SWV) with a boron-doped diamond (BDD) electrode as the working electrode and 0.1 M sulphuric acid as the supporting electrolyte. For the first time, the voltammetric profile of FLU was manifested by three irreversible and diffusion-controlled oxidation peaks at + 1.1 (P1), + 1.4 (P2) and + 1.9 V (P3). The analytical performance evaluation was assessed for all three peaks, using both pulse voltammetric techniques with the optimized operating parameters and the highest sensitivity of 1.76 nA/mu M was accomplished for P2 using DPV and 3.54 nA/mu M for P3 using SWV. The corresponding linear concentration ranges were found to be 0.99-42.9 and 4.8-35.5 mu M with the detection limits of 0.42 and 0.18 mu M, respectively. The repeatability varied..., depending on the oxidation peaks of FLU, with the relative standard deviations in the range of 3.3-8.8% and 2.9-9.3% for DPV and SWV, respectively. The proposed electrochemical platform was successfully applied in the analysis of pharmaceutical formulations, spiked human urine and water samples with the significant mean recoveries. Using BDD electrode, the current work establishes an advanced, simple and rapid alternative platform to so far used toxic mercury-based electrodes and time demanding chemically modified electrodes in cytostatic sensing. Besides, BDD electrode represents a comfortable electrochemical sensor for routine analysis in pharmaceutical, clinical and environmental chemistry. (C) 2017 Elsevier Ltd. All rights reserved.
Keywords:
cytostatic drug flutamide / boron-doped diamond electrode / electrochemical sensor / differential pulse voltammetry / square-wave voltammetrySource:
Electrochimica Acta, 2017, 251, 621-630Publisher:
- Pergamon-Elsevier Science Ltd, Oxford
Funding / projects:
- Application of advanced oxidation processes and nanostructured oxide materials for the removal of pollutants from the environment, development and optimisation of instrumental techniques for efficiency monitoring (RS-MESTD-Basic Research (BR or ON)-172030)
- Strengthening of the MagBioVin Research and Innovation Team for Development of Novel Approaches for Tumour Therapy based on Nanostructured Materials (EU-FP7-621375)
- Grant Agency of the Slovak Republic [1/0489/16]
- Grant scheme for Support of Young Researchers and Excellent Teams of Young Researchers
Note:
- Peer-reviewed manuscript: http://cherry.chem.bg.ac.rs/handle/123456789/2996
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/2997
DOI: 10.1016/j.electacta.2017.08.077
ISSN: 0013-4686
WoS: 000413004200067
Scopus: 2-s2.0-85028691293
Collections
Institution/Community
Inovacioni centar / Innovation CentreTY - JOUR AU - Šovrc, Ľubomír AU - Borovska, Katarina AU - Cinková, Kristína AU - Stanković, Dalibor AU - Planková, Alexandra PY - 2017 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2540 AB - An innovative, rapid and simple electrochemical approach for the reliable quantification of cytostatic drug flutamide (FLU) in various matrices is herein proposed. This platform involves coupling of differential pulse (DPV) and square-wave voltammetry (SWV) with a boron-doped diamond (BDD) electrode as the working electrode and 0.1 M sulphuric acid as the supporting electrolyte. For the first time, the voltammetric profile of FLU was manifested by three irreversible and diffusion-controlled oxidation peaks at + 1.1 (P1), + 1.4 (P2) and + 1.9 V (P3). The analytical performance evaluation was assessed for all three peaks, using both pulse voltammetric techniques with the optimized operating parameters and the highest sensitivity of 1.76 nA/mu M was accomplished for P2 using DPV and 3.54 nA/mu M for P3 using SWV. The corresponding linear concentration ranges were found to be 0.99-42.9 and 4.8-35.5 mu M with the detection limits of 0.42 and 0.18 mu M, respectively. The repeatability varied, depending on the oxidation peaks of FLU, with the relative standard deviations in the range of 3.3-8.8% and 2.9-9.3% for DPV and SWV, respectively. The proposed electrochemical platform was successfully applied in the analysis of pharmaceutical formulations, spiked human urine and water samples with the significant mean recoveries. Using BDD electrode, the current work establishes an advanced, simple and rapid alternative platform to so far used toxic mercury-based electrodes and time demanding chemically modified electrodes in cytostatic sensing. Besides, BDD electrode represents a comfortable electrochemical sensor for routine analysis in pharmaceutical, clinical and environmental chemistry. (C) 2017 Elsevier Ltd. All rights reserved. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Electrochimica Acta T1 - Advanced electrochemical platform for determination of cytostatic drug flutamide in various matrices using a boron-doped diamond electrode VL - 251 SP - 621 EP - 630 DO - 10.1016/j.electacta.2017.08.077 ER -
@article{ author = "Šovrc, Ľubomír and Borovska, Katarina and Cinková, Kristína and Stanković, Dalibor and Planková, Alexandra", year = "2017", abstract = "An innovative, rapid and simple electrochemical approach for the reliable quantification of cytostatic drug flutamide (FLU) in various matrices is herein proposed. This platform involves coupling of differential pulse (DPV) and square-wave voltammetry (SWV) with a boron-doped diamond (BDD) electrode as the working electrode and 0.1 M sulphuric acid as the supporting electrolyte. For the first time, the voltammetric profile of FLU was manifested by three irreversible and diffusion-controlled oxidation peaks at + 1.1 (P1), + 1.4 (P2) and + 1.9 V (P3). The analytical performance evaluation was assessed for all three peaks, using both pulse voltammetric techniques with the optimized operating parameters and the highest sensitivity of 1.76 nA/mu M was accomplished for P2 using DPV and 3.54 nA/mu M for P3 using SWV. The corresponding linear concentration ranges were found to be 0.99-42.9 and 4.8-35.5 mu M with the detection limits of 0.42 and 0.18 mu M, respectively. The repeatability varied, depending on the oxidation peaks of FLU, with the relative standard deviations in the range of 3.3-8.8% and 2.9-9.3% for DPV and SWV, respectively. The proposed electrochemical platform was successfully applied in the analysis of pharmaceutical formulations, spiked human urine and water samples with the significant mean recoveries. Using BDD electrode, the current work establishes an advanced, simple and rapid alternative platform to so far used toxic mercury-based electrodes and time demanding chemically modified electrodes in cytostatic sensing. Besides, BDD electrode represents a comfortable electrochemical sensor for routine analysis in pharmaceutical, clinical and environmental chemistry. (C) 2017 Elsevier Ltd. All rights reserved.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Electrochimica Acta", title = "Advanced electrochemical platform for determination of cytostatic drug flutamide in various matrices using a boron-doped diamond electrode", volume = "251", pages = "621-630", doi = "10.1016/j.electacta.2017.08.077" }
Šovrc, Ľ., Borovska, K., Cinková, K., Stanković, D.,& Planková, A.. (2017). Advanced electrochemical platform for determination of cytostatic drug flutamide in various matrices using a boron-doped diamond electrode. in Electrochimica Acta Pergamon-Elsevier Science Ltd, Oxford., 251, 621-630. https://doi.org/10.1016/j.electacta.2017.08.077
Šovrc Ľ, Borovska K, Cinková K, Stanković D, Planková A. Advanced electrochemical platform for determination of cytostatic drug flutamide in various matrices using a boron-doped diamond electrode. in Electrochimica Acta. 2017;251:621-630. doi:10.1016/j.electacta.2017.08.077 .
Šovrc, Ľubomír, Borovska, Katarina, Cinková, Kristína, Stanković, Dalibor, Planková, Alexandra, "Advanced electrochemical platform for determination of cytostatic drug flutamide in various matrices using a boron-doped diamond electrode" in Electrochimica Acta, 251 (2017):621-630, https://doi.org/10.1016/j.electacta.2017.08.077 . .