Leaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (Compositae) from Georgia
Аутори
Gavrilović, MilanTešević, Vele
Đorđević, Ivana S.
Rajčević, Nemanja
Bakhia, A.
Jacas, N.G.
Susanna, A.
Marin, Petar D.
Janaćković, Peđa T.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
We examined for the first time the leaf micromorphology, phytochemistry and biological activity of the rare and stenoendemic Amphoricarpos elegans Albov (Compositae) from Georgia. Scanning electron microscopy (SEM) revealed the presence of glandular trichomes on the leaves, which appeared as glandular dots that are considered the main sites of biosynthesis and accumulation of sesquiterpene lactones. Using high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) spectroscopy analyses, we identify and characterized 3-deoxyamphoricarpolide, a known sesquiterpene lactone for the genus Amphoricarpos Vis. Regarding chemotaxonomic significance, 3-deoxyamphoricarpolide represents a link between Balkan and Caucasian species of the genus. The antioxidative capacity of different leaf extracts, obtained using a Soxhlet extractor, was evaluated by two radical scavenging assays: DPPH (1,1-diphenyl-2-picrylhydrazyl) radical and the 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulp...honic acid (ABTS), and ferric ion reducing antioxidant power (FRAP). The total phenolic and flavonoid contents were also determined. The highest antioxidative activity and the highest phenolic and flavonoid contents were detected in the methanol fraction, as a result of the contribution of not only phenols, but probably also lactones. The considerable antioxidative potential indicates possible applications in pharmacy and medicine.
Кључне речи:
3-deoxyamphoricarpolide / Amphoricarpos elegans / Antioxidative activity / Glandular trichomesИзвор:
Archives of Biological Sciences, 2018, 70, 4, 613-620Финансирање / пројекти:
- Микроморфолошка, фитохемијска и молекуларна истраживања биљака - систематски, еколошки и применљиви аспекти (RS-MESTD-Basic Research (BR or ON)-173029)
- Биоактивни природни производи самониклих, гајених и јестивих биљака: одређивање структура и активности (RS-MESTD-Basic Research (BR or ON)-172053)
DOI: 10.2298/ABS180309022G
ISSN: 0354-4664
WoS: 000452187000002
Scopus: 2-s2.0-85058801815
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Gavrilović, Milan AU - Tešević, Vele AU - Đorđević, Ivana S. AU - Rajčević, Nemanja AU - Bakhia, A. AU - Jacas, N.G. AU - Susanna, A. AU - Marin, Petar D. AU - Janaćković, Peđa T. PY - 2018 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2808 AB - We examined for the first time the leaf micromorphology, phytochemistry and biological activity of the rare and stenoendemic Amphoricarpos elegans Albov (Compositae) from Georgia. Scanning electron microscopy (SEM) revealed the presence of glandular trichomes on the leaves, which appeared as glandular dots that are considered the main sites of biosynthesis and accumulation of sesquiterpene lactones. Using high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) spectroscopy analyses, we identify and characterized 3-deoxyamphoricarpolide, a known sesquiterpene lactone for the genus Amphoricarpos Vis. Regarding chemotaxonomic significance, 3-deoxyamphoricarpolide represents a link between Balkan and Caucasian species of the genus. The antioxidative capacity of different leaf extracts, obtained using a Soxhlet extractor, was evaluated by two radical scavenging assays: DPPH (1,1-diphenyl-2-picrylhydrazyl) radical and the 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), and ferric ion reducing antioxidant power (FRAP). The total phenolic and flavonoid contents were also determined. The highest antioxidative activity and the highest phenolic and flavonoid contents were detected in the methanol fraction, as a result of the contribution of not only phenols, but probably also lactones. The considerable antioxidative potential indicates possible applications in pharmacy and medicine. T2 - Archives of Biological Sciences T2 - Archives of Biological Sciences T1 - Leaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (Compositae) from Georgia VL - 70 IS - 4 SP - 613 EP - 620 DO - 10.2298/ABS180309022G ER -
@article{ author = "Gavrilović, Milan and Tešević, Vele and Đorđević, Ivana S. and Rajčević, Nemanja and Bakhia, A. and Jacas, N.G. and Susanna, A. and Marin, Petar D. and Janaćković, Peđa T.", year = "2018", abstract = "We examined for the first time the leaf micromorphology, phytochemistry and biological activity of the rare and stenoendemic Amphoricarpos elegans Albov (Compositae) from Georgia. Scanning electron microscopy (SEM) revealed the presence of glandular trichomes on the leaves, which appeared as glandular dots that are considered the main sites of biosynthesis and accumulation of sesquiterpene lactones. Using high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) spectroscopy analyses, we identify and characterized 3-deoxyamphoricarpolide, a known sesquiterpene lactone for the genus Amphoricarpos Vis. Regarding chemotaxonomic significance, 3-deoxyamphoricarpolide represents a link between Balkan and Caucasian species of the genus. The antioxidative capacity of different leaf extracts, obtained using a Soxhlet extractor, was evaluated by two radical scavenging assays: DPPH (1,1-diphenyl-2-picrylhydrazyl) radical and the 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), and ferric ion reducing antioxidant power (FRAP). The total phenolic and flavonoid contents were also determined. The highest antioxidative activity and the highest phenolic and flavonoid contents were detected in the methanol fraction, as a result of the contribution of not only phenols, but probably also lactones. The considerable antioxidative potential indicates possible applications in pharmacy and medicine.", journal = "Archives of Biological Sciences, Archives of Biological Sciences", title = "Leaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (Compositae) from Georgia", volume = "70", number = "4", pages = "613-620", doi = "10.2298/ABS180309022G" }
Gavrilović, M., Tešević, V., Đorđević, I. S., Rajčević, N., Bakhia, A., Jacas, N.G., Susanna, A., Marin, P. D.,& Janaćković, P. T.. (2018). Leaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (Compositae) from Georgia. in Archives of Biological Sciences, 70(4), 613-620. https://doi.org/10.2298/ABS180309022G
Gavrilović M, Tešević V, Đorđević IS, Rajčević N, Bakhia A, Jacas N, Susanna A, Marin PD, Janaćković PT. Leaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (Compositae) from Georgia. in Archives of Biological Sciences. 2018;70(4):613-620. doi:10.2298/ABS180309022G .
Gavrilović, Milan, Tešević, Vele, Đorđević, Ivana S., Rajčević, Nemanja, Bakhia, A., Jacas, N.G., Susanna, A., Marin, Petar D., Janaćković, Peđa T., "Leaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (Compositae) from Georgia" in Archives of Biological Sciences, 70, no. 4 (2018):613-620, https://doi.org/10.2298/ABS180309022G . .