Design and cloning strategies of recombinant allergens for diagnosis and specific immunotherapy
Апстракт
Persons suffering from allergy (Type I hypersensitivity) produce immunoglobulin E against innocuous environmental antigens such as pollen, house dust, animal dander, food proteins. Diagnosis of allergy is based on the measurement of allergen-specific IgE antibodies and on provocation with allergens in skin prick test. Diagnostic reagents based on allergen extracts obtained from natural biological material often reveal unbalanced allergen content, a presence of nonallergenic components, and are difficult to standardize. Replacement of allergen extracts with a set of individual allergens in component-resolved diagnostics is regarded as a tool for patient selection for specific immunotherapy. The concept of using single recombinant allergens to determine the patient's sensitization profile was coined "component-resolved diagnosis" and is regarded as a precondition for patient-tailored immunotherapy, i.e.,"component resolved immunotherapy"To provide reliable, more specific reagents for all...ergy diagnosis and therapy recombinant DNA technology has been widely applied. The majority of recombinant allergens by far, have been produced in the prokaryotic expression system; however eukaryotic cells (yeast, plant, insect and mammalian cells) were also exploited. To avoid side effects in the course of immunotherapy various approaches in design of hypoallergenic molecules have been performed. This chapter will give an overview of the concepts and approaches in producing recombinant allergens for component resolved diagnosis and component resolved immunotherapy. © 2014 by Nova Science Publishers, Inc. All rights reserved.
Извор:
Advances in Genetics Research, 2014, 11, 19-46Scopus: 2-s2.0-84953868156
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - CHAP AU - Grozdanović, M. AU - Popović, Milica M. AU - Gavrović-Jankulović, Marija PY - 2014 UR - https://cherry.chem.bg.ac.rs/handle/123456789/294 AB - Persons suffering from allergy (Type I hypersensitivity) produce immunoglobulin E against innocuous environmental antigens such as pollen, house dust, animal dander, food proteins. Diagnosis of allergy is based on the measurement of allergen-specific IgE antibodies and on provocation with allergens in skin prick test. Diagnostic reagents based on allergen extracts obtained from natural biological material often reveal unbalanced allergen content, a presence of nonallergenic components, and are difficult to standardize. Replacement of allergen extracts with a set of individual allergens in component-resolved diagnostics is regarded as a tool for patient selection for specific immunotherapy. The concept of using single recombinant allergens to determine the patient's sensitization profile was coined "component-resolved diagnosis" and is regarded as a precondition for patient-tailored immunotherapy, i.e.,"component resolved immunotherapy"To provide reliable, more specific reagents for allergy diagnosis and therapy recombinant DNA technology has been widely applied. The majority of recombinant allergens by far, have been produced in the prokaryotic expression system; however eukaryotic cells (yeast, plant, insect and mammalian cells) were also exploited. To avoid side effects in the course of immunotherapy various approaches in design of hypoallergenic molecules have been performed. This chapter will give an overview of the concepts and approaches in producing recombinant allergens for component resolved diagnosis and component resolved immunotherapy. © 2014 by Nova Science Publishers, Inc. All rights reserved. T2 - Advances in Genetics Research T1 - Design and cloning strategies of recombinant allergens for diagnosis and specific immunotherapy VL - 11 SP - 19 EP - 46 UR - https://hdl.handle.net/21.15107/rcub_cherry_294 ER -
@inbook{ author = "Grozdanović, M. and Popović, Milica M. and Gavrović-Jankulović, Marija", year = "2014", abstract = "Persons suffering from allergy (Type I hypersensitivity) produce immunoglobulin E against innocuous environmental antigens such as pollen, house dust, animal dander, food proteins. Diagnosis of allergy is based on the measurement of allergen-specific IgE antibodies and on provocation with allergens in skin prick test. Diagnostic reagents based on allergen extracts obtained from natural biological material often reveal unbalanced allergen content, a presence of nonallergenic components, and are difficult to standardize. Replacement of allergen extracts with a set of individual allergens in component-resolved diagnostics is regarded as a tool for patient selection for specific immunotherapy. The concept of using single recombinant allergens to determine the patient's sensitization profile was coined "component-resolved diagnosis" and is regarded as a precondition for patient-tailored immunotherapy, i.e.,"component resolved immunotherapy"To provide reliable, more specific reagents for allergy diagnosis and therapy recombinant DNA technology has been widely applied. The majority of recombinant allergens by far, have been produced in the prokaryotic expression system; however eukaryotic cells (yeast, plant, insect and mammalian cells) were also exploited. To avoid side effects in the course of immunotherapy various approaches in design of hypoallergenic molecules have been performed. This chapter will give an overview of the concepts and approaches in producing recombinant allergens for component resolved diagnosis and component resolved immunotherapy. © 2014 by Nova Science Publishers, Inc. All rights reserved.", journal = "Advances in Genetics Research", booktitle = "Design and cloning strategies of recombinant allergens for diagnosis and specific immunotherapy", volume = "11", pages = "19-46", url = "https://hdl.handle.net/21.15107/rcub_cherry_294" }
Grozdanović, M., Popović, M. M.,& Gavrović-Jankulović, M.. (2014). Design and cloning strategies of recombinant allergens for diagnosis and specific immunotherapy. in Advances in Genetics Research, 11, 19-46. https://hdl.handle.net/21.15107/rcub_cherry_294
Grozdanović M, Popović MM, Gavrović-Jankulović M. Design and cloning strategies of recombinant allergens for diagnosis and specific immunotherapy. in Advances in Genetics Research. 2014;11:19-46. https://hdl.handle.net/21.15107/rcub_cherry_294 .
Grozdanović, M., Popović, Milica M., Gavrović-Jankulović, Marija, "Design and cloning strategies of recombinant allergens for diagnosis and specific immunotherapy" in Advances in Genetics Research, 11 (2014):19-46, https://hdl.handle.net/21.15107/rcub_cherry_294 .