Determination of toxic and essential trace elements in serum of healthy and hypothyroid respondents by ICP-MS: A chemometric approach for discrimination of hypothyroidism
Аутори
Stojsavljević, AleksandarTrifković, Jelena
Rašić-Milutinović, Zorica
Jovanović, Dragana
Bogdanović, Gradimir
Mutić, Jelena
Manojlović, Dragan D.
Чланак у часопису (Рецензирана верзија)
Метаподаци
Приказ свих података о документуАпстракт
Inductively coupled plasma-mass spectrometry ((ICP-MS)) was used to determine three toxic (Ni, As, Cd) and six essential trace elements (Cr, Mn, Co, Cu, Zn, Se) in blood serum of patients with hypothyroidism (Hy group) and healthy people (control group), in order to set the experimental conditions for accurate determination of a unique profile of these elements in hypothyroidism. Method validation was performed with standard reference material of the serum by varying the sample treatment with both standard and collision mode for analysis of elements isotopes. Quadratic curvilinear functions with good performances of models and the lowest detection limits were obtained for Cr-52, Zn-66, As-75, Cd-112 in collision mode, and Mn-55, (CO)-C-59, Ni-60, Cu-65, Se-78 in standard mode. Treatment of serum samples with aqueous solution containing nitric acid, Triton X-100 and n-butanol gave the best results. Chemometric tools were applied for discrimination of patients with hypothyroidism. All ni...ne elements discriminated Hy group of samples with almost the same discriminating power as indicated by their higher values for this group of patients. Statistically significant correlation (p lt 0.01) was observed for several elements. Results indicated clear differences in element profile between Hy and control group and it could be used as a unique profile of hypothyroid state.
Кључне речи:
Trace elements / ICP-MS / Serum / HypothyroidismИзвор:
Journal of Trace Elements in Medicine and Biology, 2018, 48, 134-140Издавач:
- Elsevier Gmbh, Urban & Fischer Verlag, Jena
Финансирање / пројекти:
- Примена унапређених оксидационих процеса и наноструктурисаних оксидних материјала за уклањање загађивача из животне средине, развој и оптимизација инструменталних техника за праћење ефикасности (RS-MESTD-Basic Research (BR or ON)-172030)
Напомена:
- This is peer-reviewed version of the following article: Stojsavljević, A.; Trifković, J.; Rasić-Milutinović, Z.; Jovanović, D.; Bogdanović, G.; Mutić, J.; Manojlović, D. Determination of Toxic and Essential Trace Elements in Serum of Healthy and Hypothyroid Respondents by ICP-MS: A Chemometric Approach for Discrimination of Hypothyroidism. Journal of Trace Elements in Medicine and Biology 2018, 48, 134–140. https://doi.org/10.1016/j.jtemb.2018.03.020
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3075
DOI: 10.1016/j.jtemb.2018.03.020
ISSN: 0946-672X
PubMed: 29773171
WoS: 000438326700020
Scopus: 2-s2.0-85044478279
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Stojsavljević, Aleksandar AU - Trifković, Jelena AU - Rašić-Milutinović, Zorica AU - Jovanović, Dragana AU - Bogdanović, Gradimir AU - Mutić, Jelena AU - Manojlović, Dragan D. PY - 2018 UR - https://cherry.chem.bg.ac.rs/handle/123456789/3074 AB - Inductively coupled plasma-mass spectrometry ((ICP-MS)) was used to determine three toxic (Ni, As, Cd) and six essential trace elements (Cr, Mn, Co, Cu, Zn, Se) in blood serum of patients with hypothyroidism (Hy group) and healthy people (control group), in order to set the experimental conditions for accurate determination of a unique profile of these elements in hypothyroidism. Method validation was performed with standard reference material of the serum by varying the sample treatment with both standard and collision mode for analysis of elements isotopes. Quadratic curvilinear functions with good performances of models and the lowest detection limits were obtained for Cr-52, Zn-66, As-75, Cd-112 in collision mode, and Mn-55, (CO)-C-59, Ni-60, Cu-65, Se-78 in standard mode. Treatment of serum samples with aqueous solution containing nitric acid, Triton X-100 and n-butanol gave the best results. Chemometric tools were applied for discrimination of patients with hypothyroidism. All nine elements discriminated Hy group of samples with almost the same discriminating power as indicated by their higher values for this group of patients. Statistically significant correlation (p lt 0.01) was observed for several elements. Results indicated clear differences in element profile between Hy and control group and it could be used as a unique profile of hypothyroid state. PB - Elsevier Gmbh, Urban & Fischer Verlag, Jena T2 - Journal of Trace Elements in Medicine and Biology T1 - Determination of toxic and essential trace elements in serum of healthy and hypothyroid respondents by ICP-MS: A chemometric approach for discrimination of hypothyroidism VL - 48 SP - 134 EP - 140 DO - 10.1016/j.jtemb.2018.03.020 ER -
@article{ author = "Stojsavljević, Aleksandar and Trifković, Jelena and Rašić-Milutinović, Zorica and Jovanović, Dragana and Bogdanović, Gradimir and Mutić, Jelena and Manojlović, Dragan D.", year = "2018", abstract = "Inductively coupled plasma-mass spectrometry ((ICP-MS)) was used to determine three toxic (Ni, As, Cd) and six essential trace elements (Cr, Mn, Co, Cu, Zn, Se) in blood serum of patients with hypothyroidism (Hy group) and healthy people (control group), in order to set the experimental conditions for accurate determination of a unique profile of these elements in hypothyroidism. Method validation was performed with standard reference material of the serum by varying the sample treatment with both standard and collision mode for analysis of elements isotopes. Quadratic curvilinear functions with good performances of models and the lowest detection limits were obtained for Cr-52, Zn-66, As-75, Cd-112 in collision mode, and Mn-55, (CO)-C-59, Ni-60, Cu-65, Se-78 in standard mode. Treatment of serum samples with aqueous solution containing nitric acid, Triton X-100 and n-butanol gave the best results. Chemometric tools were applied for discrimination of patients with hypothyroidism. All nine elements discriminated Hy group of samples with almost the same discriminating power as indicated by their higher values for this group of patients. Statistically significant correlation (p lt 0.01) was observed for several elements. Results indicated clear differences in element profile between Hy and control group and it could be used as a unique profile of hypothyroid state.", publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena", journal = "Journal of Trace Elements in Medicine and Biology", title = "Determination of toxic and essential trace elements in serum of healthy and hypothyroid respondents by ICP-MS: A chemometric approach for discrimination of hypothyroidism", volume = "48", pages = "134-140", doi = "10.1016/j.jtemb.2018.03.020" }
Stojsavljević, A., Trifković, J., Rašić-Milutinović, Z., Jovanović, D., Bogdanović, G., Mutić, J.,& Manojlović, D. D.. (2018). Determination of toxic and essential trace elements in serum of healthy and hypothyroid respondents by ICP-MS: A chemometric approach for discrimination of hypothyroidism. in Journal of Trace Elements in Medicine and Biology Elsevier Gmbh, Urban & Fischer Verlag, Jena., 48, 134-140. https://doi.org/10.1016/j.jtemb.2018.03.020
Stojsavljević A, Trifković J, Rašić-Milutinović Z, Jovanović D, Bogdanović G, Mutić J, Manojlović DD. Determination of toxic and essential trace elements in serum of healthy and hypothyroid respondents by ICP-MS: A chemometric approach for discrimination of hypothyroidism. in Journal of Trace Elements in Medicine and Biology. 2018;48:134-140. doi:10.1016/j.jtemb.2018.03.020 .
Stojsavljević, Aleksandar, Trifković, Jelena, Rašić-Milutinović, Zorica, Jovanović, Dragana, Bogdanović, Gradimir, Mutić, Jelena, Manojlović, Dragan D., "Determination of toxic and essential trace elements in serum of healthy and hypothyroid respondents by ICP-MS: A chemometric approach for discrimination of hypothyroidism" in Journal of Trace Elements in Medicine and Biology, 48 (2018):134-140, https://doi.org/10.1016/j.jtemb.2018.03.020 . .