A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-: Tert -butylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai
Authors
Medić, AnaStojanović, Ksenija A.
Izrael-Živković, Lidija
Beškoski, Vladimir
Lončarević, Branka D.
Kazazić, Saša
Karadžić, Ivanka M.
Article (Published version)
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The Pseudomonas aeruginosa san ai strain was investigated for its capability to degrade the 2,6-di-tert-butylphenol (2,6-DTBP) plastic additive, a hazardous and toxic substance for aquatic life. This investigation was performed under different parameter values: 2,6-DTBP concentration, inoculum size, pH, and temperature. The GC-MS study showed that P. aeruginosa efficiently degraded 2,6-DTBP in the pH range of 5-8 at higher temperatures. Under exposure to 2,6-DTBP concentrations of 2, 10, and 100 mg L-1, the strain degraded by 100, 100, and 85%, respectively, for 7 days. Crude enzyme preparation from the biomass of P. aeruginosa san ai showed higher efficiency in 2,6-DTBP removal than that shown by whole microbial cells. Gene encoding for the enzymes involved in the degradation of aromatic compounds in P. aeruginosa san ai was identified. To complement the genomic data, a comparative proteomic study of P. aeruginosa san ai grown on 2,6-DTBP or sunflower oil was conducted by means of nan...oLC-MS/MS. The presence of aromatic substances resulted in the upregulation of aromatic ring cleavage enzymes, whose activity was confirmed by enzymatic tests; therefore, it could be concluded that 2,6-DTBP might be degraded by ortho-ring cleavage. A comparative proteomics study of P. aeruginosa san ai indicated that the core molecular responses to aromatic substances can be summarized as the upregulation of proteins responsible for amino acid metabolism with emphasized glutamate metabolism and energy production with upregulated enzymes of glyoxylate bypass. P. aeruginosa san ai has a high capacity to efficiently degrade aromatic compounds, and therefore its whole cells or enzymes could be used in the treatment of contaminated areas.
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RSC Advances, 2019, 9, 41, 23696-23710Funding / projects:
- Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43004)
- Geochemical investigations of sedimentary rocks - fossil fuels and environmental pollutants (RS-MESTD-Basic Research (BR or ON)-176006)
Note:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3347
DOI: 10.1039/C9RA04298A
ISSN: 2046-2069
WoS: 000478947000030
Scopus: 2-s2.0-85070413922
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Medić, Ana AU - Stojanović, Ksenija A. AU - Izrael-Živković, Lidija AU - Beškoski, Vladimir AU - Lončarević, Branka D. AU - Kazazić, Saša AU - Karadžić, Ivanka M. PY - 2019 UR - https://cherry.chem.bg.ac.rs/handle/123456789/3346 AB - The Pseudomonas aeruginosa san ai strain was investigated for its capability to degrade the 2,6-di-tert-butylphenol (2,6-DTBP) plastic additive, a hazardous and toxic substance for aquatic life. This investigation was performed under different parameter values: 2,6-DTBP concentration, inoculum size, pH, and temperature. The GC-MS study showed that P. aeruginosa efficiently degraded 2,6-DTBP in the pH range of 5-8 at higher temperatures. Under exposure to 2,6-DTBP concentrations of 2, 10, and 100 mg L-1, the strain degraded by 100, 100, and 85%, respectively, for 7 days. Crude enzyme preparation from the biomass of P. aeruginosa san ai showed higher efficiency in 2,6-DTBP removal than that shown by whole microbial cells. Gene encoding for the enzymes involved in the degradation of aromatic compounds in P. aeruginosa san ai was identified. To complement the genomic data, a comparative proteomic study of P. aeruginosa san ai grown on 2,6-DTBP or sunflower oil was conducted by means of nanoLC-MS/MS. The presence of aromatic substances resulted in the upregulation of aromatic ring cleavage enzymes, whose activity was confirmed by enzymatic tests; therefore, it could be concluded that 2,6-DTBP might be degraded by ortho-ring cleavage. A comparative proteomics study of P. aeruginosa san ai indicated that the core molecular responses to aromatic substances can be summarized as the upregulation of proteins responsible for amino acid metabolism with emphasized glutamate metabolism and energy production with upregulated enzymes of glyoxylate bypass. P. aeruginosa san ai has a high capacity to efficiently degrade aromatic compounds, and therefore its whole cells or enzymes could be used in the treatment of contaminated areas. T2 - RSC Advances T1 - A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-: Tert -butylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai VL - 9 IS - 41 SP - 23696 EP - 23710 DO - 10.1039/C9RA04298A ER -
@article{ author = "Medić, Ana and Stojanović, Ksenija A. and Izrael-Živković, Lidija and Beškoski, Vladimir and Lončarević, Branka D. and Kazazić, Saša and Karadžić, Ivanka M.", year = "2019", abstract = "The Pseudomonas aeruginosa san ai strain was investigated for its capability to degrade the 2,6-di-tert-butylphenol (2,6-DTBP) plastic additive, a hazardous and toxic substance for aquatic life. This investigation was performed under different parameter values: 2,6-DTBP concentration, inoculum size, pH, and temperature. The GC-MS study showed that P. aeruginosa efficiently degraded 2,6-DTBP in the pH range of 5-8 at higher temperatures. Under exposure to 2,6-DTBP concentrations of 2, 10, and 100 mg L-1, the strain degraded by 100, 100, and 85%, respectively, for 7 days. Crude enzyme preparation from the biomass of P. aeruginosa san ai showed higher efficiency in 2,6-DTBP removal than that shown by whole microbial cells. Gene encoding for the enzymes involved in the degradation of aromatic compounds in P. aeruginosa san ai was identified. To complement the genomic data, a comparative proteomic study of P. aeruginosa san ai grown on 2,6-DTBP or sunflower oil was conducted by means of nanoLC-MS/MS. The presence of aromatic substances resulted in the upregulation of aromatic ring cleavage enzymes, whose activity was confirmed by enzymatic tests; therefore, it could be concluded that 2,6-DTBP might be degraded by ortho-ring cleavage. A comparative proteomics study of P. aeruginosa san ai indicated that the core molecular responses to aromatic substances can be summarized as the upregulation of proteins responsible for amino acid metabolism with emphasized glutamate metabolism and energy production with upregulated enzymes of glyoxylate bypass. P. aeruginosa san ai has a high capacity to efficiently degrade aromatic compounds, and therefore its whole cells or enzymes could be used in the treatment of contaminated areas.", journal = "RSC Advances", title = "A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-: Tert -butylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai", volume = "9", number = "41", pages = "23696-23710", doi = "10.1039/C9RA04298A" }
Medić, A., Stojanović, K. A., Izrael-Živković, L., Beškoski, V., Lončarević, B. D., Kazazić, S.,& Karadžić, I. M.. (2019). A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-: Tert -butylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai. in RSC Advances, 9(41), 23696-23710. https://doi.org/10.1039/C9RA04298A
Medić A, Stojanović KA, Izrael-Živković L, Beškoski V, Lončarević BD, Kazazić S, Karadžić IM. A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-: Tert -butylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai. in RSC Advances. 2019;9(41):23696-23710. doi:10.1039/C9RA04298A .
Medić, Ana, Stojanović, Ksenija A., Izrael-Živković, Lidija, Beškoski, Vladimir, Lončarević, Branka D., Kazazić, Saša, Karadžić, Ivanka M., "A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-: Tert -butylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai" in RSC Advances, 9, no. 41 (2019):23696-23710, https://doi.org/10.1039/C9RA04298A . .