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Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model
dc.creator | Nešić, Andrijana N. | |
dc.creator | Stam, Annemarie | |
dc.creator | Čavić, Milena | |
dc.creator | Ten Klooster, Jean Paul | |
dc.creator | Pieters, Raymond | |
dc.creator | Smit, Joost | |
dc.creator | Gavrović-Jankulović, Marija | |
dc.date.accessioned | 2019-10-01T11:28:39Z | |
dc.date.available | 2019-10-01T11:28:39Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 1756-4646 | |
dc.identifier.uri | https://cherry.chem.bg.ac.rs/handle/123456789/3485 | |
dc.description.abstract | In this study, two intestinal models were employed to assess the modulatory potential of a major kiwifruit allergen on the innate immunity of epithelial cells. Effects of Act d 1 were analyzed in terms of gene expression and structural changes of tight junction (TJ) proteins, as well as up-regulation of pro-inflammatory cytokines in Caco-2 cells and, for the first time, in mouse-derived intestinal 2-dimensional (2D) organoids. Biologically active Act d 1 induced up-regulation of TJ genes for CLDN-2, CLDN-3, CLDN-4, ZO-1, and on the protein level induced release of pro-inflammatory cytokines IL-1β, TNFα and IL-33 in both employed model systems. In 2D-organoids, active Act d 1 impaired the TJ protein networks of E-cadherin, claudin-3, and ZO-1. 2D-organoids generated from mouse intestine are a promising new model system for the assessment of allergen-induced intestinal cell responses and a useful tool for mitigation of risks associated with novel food proteins. | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS// | |
dc.relation | Institute for Risk Assessment Sciences, University of Utrecht,Netherlands, which was supported by COST Action FA1402. | |
dc.rights | restrictedAccess | |
dc.source | Journal of Functional Foods | |
dc.subject | Act d 1 | |
dc.subject | Cysteine protease | |
dc.subject | Food allergy | |
dc.subject | Pro-inflammatory cytokines | |
dc.subject | TJ proteins | |
dc.title | Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model | |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Смит, Јоост; Пиетерс, Раyмонд; Тен Клоостер, Јеан Паул; Чавић, Милена; Нешић, Aндријана; Стам, Aннемарие; Гавровић-Јанкуловић, Марија; | |
dc.citation.volume | 62 | |
dc.identifier.wos | 000491207500030 | |
dc.identifier.doi | 10.1016/j.jff.2019.103556 | |
dc.citation.rank | M21~ | |
dc.type.version | publishedVersion | |
dc.identifier.scopus | 2-s2.0-85071845404 |