Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium
Samo za registrovane korisnike
2019
Autori
Gojgić-Cvijović, Gordana D.Jakovljević, Dragica M.
Lončarević, Branka D.
Todorović, Nevena M.
Pergal, Marija V.
Ćirić, J.
Loos, K.
Beškoski, Vladimir
Vrvić, Miroslav M.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100–140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L of total sugars, as well as K2HPO4. A levan yield of 53.2 g/L was obtained on a medium optimized by response surface methodology, containing 62.6% of sugar originating from molasses, and 4.66 g/L of phosphate, with initial pH value of 7.2. In comparison to the media with 200 and 400 g/L sucrose, in the molasses optimized medium, the observed bacterial growth was faster, while the maximum production of polysaccharide was achieved over a shorter time interval (48 h). The polysaccharide produced in molasses medium had a weight average molecular weight of 5.82 × 106 Da, degree of branching 12.68%, viscosity of 0.24 dL/g, and based on methylation analysis and NMR data, it did not significantly differ from levan obtained in the medium ...with 200 g/L sucrose. © 2018 Elsevier B.V.
Ključne reči:
Levan / Molasses / Molecular weightIzvor:
International Journal of Biological Macromolecules, 2019, 121, 142-151Finansiranje / projekti:
- Simultana bioremedijacija i soilifikacija degradiranih prostora, za očuvanje prirodnih resursa biološki aktivnih supstanci i razvoj i proizvodnju biomaterijala i dijetetskih proizvoda (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43004)
- Bioaktivni prirodni proizvodi samoniklih, gajenih i jestivih biljaka: određivanje struktura i aktivnosti (RS-MESTD-Basic Research (BR or ON)-172053)
- Sinteza i karakterizacija novih funkcionalnih polimera i polimernih nanokompozita (RS-MESTD-Basic Research (BR or ON)-172062)
Napomena:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/2943
- Peer reviewed manuscript: http://cherry.chem.bg.ac.rs/handle/123456789/2799
DOI: 10.1016/j.ijbiomac.2018.10.019
ISSN: 0141-8130
WoS: 000452346100018
Scopus: 2-s2.0-85054437248
Kolekcije
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Gojgić-Cvijović, Gordana D. AU - Jakovljević, Dragica M. AU - Lončarević, Branka D. AU - Todorović, Nevena M. AU - Pergal, Marija V. AU - Ćirić, J. AU - Loos, K. AU - Beškoski, Vladimir AU - Vrvić, Miroslav M. PY - 2019 UR - https://cherry.chem.bg.ac.rs/handle/123456789/350 AB - The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100–140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L of total sugars, as well as K2HPO4. A levan yield of 53.2 g/L was obtained on a medium optimized by response surface methodology, containing 62.6% of sugar originating from molasses, and 4.66 g/L of phosphate, with initial pH value of 7.2. In comparison to the media with 200 and 400 g/L sucrose, in the molasses optimized medium, the observed bacterial growth was faster, while the maximum production of polysaccharide was achieved over a shorter time interval (48 h). The polysaccharide produced in molasses medium had a weight average molecular weight of 5.82 × 106 Da, degree of branching 12.68%, viscosity of 0.24 dL/g, and based on methylation analysis and NMR data, it did not significantly differ from levan obtained in the medium with 200 g/L sucrose. © 2018 Elsevier B.V. T2 - International Journal of Biological Macromolecules T1 - Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium VL - 121 SP - 142 EP - 151 DO - 10.1016/j.ijbiomac.2018.10.019 ER -
@article{ author = "Gojgić-Cvijović, Gordana D. and Jakovljević, Dragica M. and Lončarević, Branka D. and Todorović, Nevena M. and Pergal, Marija V. and Ćirić, J. and Loos, K. and Beškoski, Vladimir and Vrvić, Miroslav M.", year = "2019", abstract = "The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100–140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L of total sugars, as well as K2HPO4. A levan yield of 53.2 g/L was obtained on a medium optimized by response surface methodology, containing 62.6% of sugar originating from molasses, and 4.66 g/L of phosphate, with initial pH value of 7.2. In comparison to the media with 200 and 400 g/L sucrose, in the molasses optimized medium, the observed bacterial growth was faster, while the maximum production of polysaccharide was achieved over a shorter time interval (48 h). The polysaccharide produced in molasses medium had a weight average molecular weight of 5.82 × 106 Da, degree of branching 12.68%, viscosity of 0.24 dL/g, and based on methylation analysis and NMR data, it did not significantly differ from levan obtained in the medium with 200 g/L sucrose. © 2018 Elsevier B.V.", journal = "International Journal of Biological Macromolecules", title = "Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium", volume = "121", pages = "142-151", doi = "10.1016/j.ijbiomac.2018.10.019" }
Gojgić-Cvijović, G. D., Jakovljević, D. M., Lončarević, B. D., Todorović, N. M., Pergal, M. V., Ćirić, J., Loos, K., Beškoski, V.,& Vrvić, M. M.. (2019). Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium. in International Journal of Biological Macromolecules, 121, 142-151. https://doi.org/10.1016/j.ijbiomac.2018.10.019
Gojgić-Cvijović GD, Jakovljević DM, Lončarević BD, Todorović NM, Pergal MV, Ćirić J, Loos K, Beškoski V, Vrvić MM. Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium. in International Journal of Biological Macromolecules. 2019;121:142-151. doi:10.1016/j.ijbiomac.2018.10.019 .
Gojgić-Cvijović, Gordana D., Jakovljević, Dragica M., Lončarević, Branka D., Todorović, Nevena M., Pergal, Marija V., Ćirić, J., Loos, K., Beškoski, Vladimir, Vrvić, Miroslav M., "Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium" in International Journal of Biological Macromolecules, 121 (2019):142-151, https://doi.org/10.1016/j.ijbiomac.2018.10.019 . .