Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees
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Autori
Kojić, DanijelaPurać, Jelena
Nikolić, Tatjana V.
Orčić, Snežana M.
Vujanović, Dragana
Ilijević, Konstantin
Vukašinović, Elvira L.
Blagojević, Duško P.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Over the past decades, the number of managed honey bee Apis mellifera L. (Hymenoptera: Apidae) colonies have been decreasing. The majority of losses occur during winter, suggesting that overwintering honey bees are more susceptible to adverse factors. We focused on the oxidative status of overwintering honey bees, particularly at the beginning (November) and end (March) of the wintering period. Colonies from three locations with different anthropogenic influences were selected: Belgrade, an urban zone, Zajača, an industrial zone, and Susek, a rural area. We measured levels of malondialdehyde (MDA), as a marker of lipid peroxidation, as well as the expression and activity of select antioxidative enzymes: Superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST). Our results show that enzyme activity and gene expression of antioxidative enzymes are influenced by both sample location and the time of sampling. The majority of analyzed genes had significantly reduced ex...pression, at the end of the overwintering period when higher activities of antioxidative enzymes were also recorded. Among the analyzed parameters, SOD activity and gene expression of microsomal GST isoforms were more affected by local environmental conditions, suggesting the complex role of these enzymes in antioxidative defense and detoxification. The higher MDA levels observed at the end of overwintering for all three locations likely reflects accumulated oxidative damage which could be associated with the aging process, brood rearing and/or the onset flying activity.
Ključne reči:
Apidae / Catalase / Glutathione S-transferase / Malondialdehyde / QPCR / Superoxide dismutaseIzvor:
Entomologia Generalis, 2019, 39, 1, 33-44Izdavač:
- E. Schweizerbart'sche Verlagsbuchhandlung
Finansiranje / projekti:
- Molekularni mehanizmi redoks signalinga u homeostazi, adaptaciji i patologiji (RS-MESTD-Basic Research (BR or ON)-173014)
DOI: 10.1127/entomologia/2019/0743
ISSN: 0171-8177
WoS: 000478952500004
Scopus: 2-s2.0-85070656995
Kolekcije
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Kojić, Danijela AU - Purać, Jelena AU - Nikolić, Tatjana V. AU - Orčić, Snežana M. AU - Vujanović, Dragana AU - Ilijević, Konstantin AU - Vukašinović, Elvira L. AU - Blagojević, Duško P. PY - 2019 UR - https://cherry.chem.bg.ac.rs/handle/123456789/3734 AB - Over the past decades, the number of managed honey bee Apis mellifera L. (Hymenoptera: Apidae) colonies have been decreasing. The majority of losses occur during winter, suggesting that overwintering honey bees are more susceptible to adverse factors. We focused on the oxidative status of overwintering honey bees, particularly at the beginning (November) and end (March) of the wintering period. Colonies from three locations with different anthropogenic influences were selected: Belgrade, an urban zone, Zajača, an industrial zone, and Susek, a rural area. We measured levels of malondialdehyde (MDA), as a marker of lipid peroxidation, as well as the expression and activity of select antioxidative enzymes: Superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST). Our results show that enzyme activity and gene expression of antioxidative enzymes are influenced by both sample location and the time of sampling. The majority of analyzed genes had significantly reduced expression, at the end of the overwintering period when higher activities of antioxidative enzymes were also recorded. Among the analyzed parameters, SOD activity and gene expression of microsomal GST isoforms were more affected by local environmental conditions, suggesting the complex role of these enzymes in antioxidative defense and detoxification. The higher MDA levels observed at the end of overwintering for all three locations likely reflects accumulated oxidative damage which could be associated with the aging process, brood rearing and/or the onset flying activity. PB - E. Schweizerbart'sche Verlagsbuchhandlung T2 - Entomologia Generalis T1 - Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees VL - 39 IS - 1 SP - 33 EP - 44 DO - 10.1127/entomologia/2019/0743 ER -
@article{ author = "Kojić, Danijela and Purać, Jelena and Nikolić, Tatjana V. and Orčić, Snežana M. and Vujanović, Dragana and Ilijević, Konstantin and Vukašinović, Elvira L. and Blagojević, Duško P.", year = "2019", abstract = "Over the past decades, the number of managed honey bee Apis mellifera L. (Hymenoptera: Apidae) colonies have been decreasing. The majority of losses occur during winter, suggesting that overwintering honey bees are more susceptible to adverse factors. We focused on the oxidative status of overwintering honey bees, particularly at the beginning (November) and end (March) of the wintering period. Colonies from three locations with different anthropogenic influences were selected: Belgrade, an urban zone, Zajača, an industrial zone, and Susek, a rural area. We measured levels of malondialdehyde (MDA), as a marker of lipid peroxidation, as well as the expression and activity of select antioxidative enzymes: Superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST). Our results show that enzyme activity and gene expression of antioxidative enzymes are influenced by both sample location and the time of sampling. The majority of analyzed genes had significantly reduced expression, at the end of the overwintering period when higher activities of antioxidative enzymes were also recorded. Among the analyzed parameters, SOD activity and gene expression of microsomal GST isoforms were more affected by local environmental conditions, suggesting the complex role of these enzymes in antioxidative defense and detoxification. The higher MDA levels observed at the end of overwintering for all three locations likely reflects accumulated oxidative damage which could be associated with the aging process, brood rearing and/or the onset flying activity.", publisher = "E. Schweizerbart'sche Verlagsbuchhandlung", journal = "Entomologia Generalis", title = "Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees", volume = "39", number = "1", pages = "33-44", doi = "10.1127/entomologia/2019/0743" }
Kojić, D., Purać, J., Nikolić, T. V., Orčić, S. M., Vujanović, D., Ilijević, K., Vukašinović, E. L.,& Blagojević, D. P.. (2019). Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees. in Entomologia Generalis E. Schweizerbart'sche Verlagsbuchhandlung., 39(1), 33-44. https://doi.org/10.1127/entomologia/2019/0743
Kojić D, Purać J, Nikolić TV, Orčić SM, Vujanović D, Ilijević K, Vukašinović EL, Blagojević DP. Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees. in Entomologia Generalis. 2019;39(1):33-44. doi:10.1127/entomologia/2019/0743 .
Kojić, Danijela, Purać, Jelena, Nikolić, Tatjana V., Orčić, Snežana M., Vujanović, Dragana, Ilijević, Konstantin, Vukašinović, Elvira L., Blagojević, Duško P., "Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees" in Entomologia Generalis, 39, no. 1 (2019):33-44, https://doi.org/10.1127/entomologia/2019/0743 . .