Thin-layer chromatography of several antihypertensive drugs from the group of angiotensin converting enzyme inhibitors

Abstract

A rapid and simple method for the chromatographic separation of pharmacologically active components contained in some antihypertensive drugs has been developed employing thin-layers of silica gel and polyacrylonitrile sorbent (PANS). The active compounds of Captopril - (S)-1-(3-mercapto-2-methyl-1-oxopropyl)-L-proline, Enalapril - (S)-1[N-[1(ethoxycarbonyl-3- phenylpropyl-L-alanyl]-L-proline, Lisinopril - (S) - 1-[N-2-(carboxy-3-phenylpropyl)-L-lysyl]-L-proline, Quinapril - [3S-[2[R*(R*)],3R*]], -2- [2-[[1(-ethoxycarbonyl)-3-phenylpropyl]amino]-1-oxopropyl]-1,2,3,4-tetrahydro-3-iso-quinoline-carboxylic acid, Ramipril- [2S[1[R*(R*)],2 alpha 3a beta ,6a beta]]-1[2[[1-(ethoxycarbonyl)-3-phenylpropyl]amino]-1-oxopropyl]octahydrocyclopenta[b]-pyrole-2-carboxylic acid and Cilazapril - [1S-[1 alpha ,9 alpha (R*)]]- 9-[[1-(ethoxycarbonyl)-3-phenylpropyl]amino] octahydro-10-oxo-6H-pyridazino[1,2-a][1,2] diazepine-1-carboxylic acid, were successfully separated by the presented procedures. For their chromatographic separation on silica gel sixteen and on PANS thirteen solvents were used.

U radu je u uslovima hromatografije na tankim slojevima silika-gela i poliakrilonitrilnog sorbenta (PANS-a), izvršeno razdvajanje sledećih antihipertenziva iz grupe angiotenzin konvertujućih enzim inhibitora (ACE inhibitora): Kaptopril-a, Enalapril-a, Lizinopril-a, Kvinapril-a, Ramipril-a, Cilazapril-a. Za njihovo hromatografsko razdvajanje upotrebljeni su mnogi rastvarači. Na osnovu dobijenih rezultata diskutovani su mehanizmi odvajanja. Na taj način data je jedna veoma brza i jednostavna metoda za odvajanje pomenutih supstanci.