IgM and IgG Immunoreactivity of SARS-CoV-2 Recombinant M Protein
Аутори
Lopandić, ZoranaProtić-Rosić, Isidora
Todorović, Aleksandra
Glamočlija, Sofija
Gnjatović, Marija Lj.
Ćujic, Danica
Gavrović-Jankulović, Marija
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Diagnostic evaluation of specific antibodies against the SARS-CoV-2 virus is mainly based on spike (S) and nucleocapsid (N) proteins. Despite the critical functions in virus infection and contribution to the pattern of immunodominance in COVID-19, exploitation of the most abundant membrane (M) protein in the SARS-CoV-2 serology tests is minimal. This study investigated the recombinant M protein’s immunoreactivity with the sera from COVID-19 convalescents. In silico designed protein was created from the outer N-terminal part (19 aa) and internal C-terminal tail (101–222 aa) of the M protein (YP_009724393.1) and was recombinantly produced and purified. The designed M protein (16,498.74 Da, pI 8.79) revealed both IgM and IgG reactivity with serum samples from COVID-19 convalescents in Western blot. In ELISA, more than 93% (28/30) of COVID-19 sera were positive for IgM detection, and more than 96% (29/30) were positive for specific IgG detection to M protein. Based on the capacity to provo...ke an immune response and its strong antigenic properties, as shown here, and the fact that it is also involved in the virion entry into host cells, the M protein of the SARS-CoV-2 virus as a good antigen has the potential in diagnostic purposes and vaccine design.
Кључне речи:
antigenicity / COVID-19 / ELISA / IgG reactivity / IgM reactivity / SARS-CoV-2 membrane proteinИзвор:
International Journal of Molecular Sciences, 2021, 22, 9, 4951-Издавач:
- MDPI
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200288 (Иновациони центар Хемијског факултета у Београду доо) (RS-200288)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200019 (Универзитет у Београду, Институт за примену нуклеарне енергије - ИНЕП) (RS-200019)
DOI: 10.3390/ijms22094951
ISSN: 1422-0067
PubMed: 34066920
WoS: 000650390300001
Scopus: 2-s2.0-85105387335
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Lopandić, Zorana AU - Protić-Rosić, Isidora AU - Todorović, Aleksandra AU - Glamočlija, Sofija AU - Gnjatović, Marija Lj. AU - Ćujic, Danica AU - Gavrović-Jankulović, Marija PY - 2021 UR - http://cherry.chem.bg.ac.rs/handle/123456789/4800 AB - Diagnostic evaluation of specific antibodies against the SARS-CoV-2 virus is mainly based on spike (S) and nucleocapsid (N) proteins. Despite the critical functions in virus infection and contribution to the pattern of immunodominance in COVID-19, exploitation of the most abundant membrane (M) protein in the SARS-CoV-2 serology tests is minimal. This study investigated the recombinant M protein’s immunoreactivity with the sera from COVID-19 convalescents. In silico designed protein was created from the outer N-terminal part (19 aa) and internal C-terminal tail (101–222 aa) of the M protein (YP_009724393.1) and was recombinantly produced and purified. The designed M protein (16,498.74 Da, pI 8.79) revealed both IgM and IgG reactivity with serum samples from COVID-19 convalescents in Western blot. In ELISA, more than 93% (28/30) of COVID-19 sera were positive for IgM detection, and more than 96% (29/30) were positive for specific IgG detection to M protein. Based on the capacity to provoke an immune response and its strong antigenic properties, as shown here, and the fact that it is also involved in the virion entry into host cells, the M protein of the SARS-CoV-2 virus as a good antigen has the potential in diagnostic purposes and vaccine design. PB - MDPI T2 - International Journal of Molecular Sciences T1 - IgM and IgG Immunoreactivity of SARS-CoV-2 Recombinant M Protein VL - 22 IS - 9 SP - 4951 DO - 10.3390/ijms22094951 ER -
@article{ author = "Lopandić, Zorana and Protić-Rosić, Isidora and Todorović, Aleksandra and Glamočlija, Sofija and Gnjatović, Marija Lj. and Ćujic, Danica and Gavrović-Jankulović, Marija", year = "2021", abstract = "Diagnostic evaluation of specific antibodies against the SARS-CoV-2 virus is mainly based on spike (S) and nucleocapsid (N) proteins. Despite the critical functions in virus infection and contribution to the pattern of immunodominance in COVID-19, exploitation of the most abundant membrane (M) protein in the SARS-CoV-2 serology tests is minimal. This study investigated the recombinant M protein’s immunoreactivity with the sera from COVID-19 convalescents. In silico designed protein was created from the outer N-terminal part (19 aa) and internal C-terminal tail (101–222 aa) of the M protein (YP_009724393.1) and was recombinantly produced and purified. The designed M protein (16,498.74 Da, pI 8.79) revealed both IgM and IgG reactivity with serum samples from COVID-19 convalescents in Western blot. In ELISA, more than 93% (28/30) of COVID-19 sera were positive for IgM detection, and more than 96% (29/30) were positive for specific IgG detection to M protein. Based on the capacity to provoke an immune response and its strong antigenic properties, as shown here, and the fact that it is also involved in the virion entry into host cells, the M protein of the SARS-CoV-2 virus as a good antigen has the potential in diagnostic purposes and vaccine design.", publisher = "MDPI", journal = "International Journal of Molecular Sciences", title = "IgM and IgG Immunoreactivity of SARS-CoV-2 Recombinant M Protein", volume = "22", number = "9", pages = "4951", doi = "10.3390/ijms22094951" }
Lopandić, Z., Protić-Rosić, I., Todorović, A., Glamočlija, S., Gnjatović, M. Lj., Ćujic, D.,& Gavrović-Jankulović, M.. (2021). IgM and IgG Immunoreactivity of SARS-CoV-2 Recombinant M Protein. in International Journal of Molecular Sciences MDPI., 22(9), 4951. https://doi.org/10.3390/ijms22094951
Lopandić Z, Protić-Rosić I, Todorović A, Glamočlija S, Gnjatović ML, Ćujic D, Gavrović-Jankulović M. IgM and IgG Immunoreactivity of SARS-CoV-2 Recombinant M Protein. in International Journal of Molecular Sciences. 2021;22(9):4951. doi:10.3390/ijms22094951 .
Lopandić, Zorana, Protić-Rosić, Isidora, Todorović, Aleksandra, Glamočlija, Sofija, Gnjatović, Marija Lj., Ćujic, Danica, Gavrović-Jankulović, Marija, "IgM and IgG Immunoreactivity of SARS-CoV-2 Recombinant M Protein" in International Journal of Molecular Sciences, 22, no. 9 (2021):4951, https://doi.org/10.3390/ijms22094951 . .