Analytical Protocols in Phycobiliproteins Analysis
Само за регистроване кориснике
2020
Аутори
Nikolić, MilanMinić, Simeon L.
Mačvanin, Mirjana T.
Stanić-Vučinić, Dragana
Ćirković-Veličković, Tanja
Jacob-Lopes, Eduardo
Queiroz, Maria Isabel
Zepka, Leila Queiroz
Поглавље у монографији (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of this chapter is to review and discuss methodology and protocols in the analysis of phycobiliproteins (phycocyanins, allophycocyanins, and phycoerythrins) and their chromophores. Due to the presence of multiple covalently bound open-chain tetrapyrrole chromophores, phycobiliproteins are colored and strongly fluorescent molecules, with high absorption coefficients (105 to 106) and excellent fluorescent quantum yield (0.51 up to 0.98). Therefore, a vast number of methods for phycobiliproteins analysis is based on these spectral characteristics, whereas assessment of their bioactivity is related to their exceptional redox and metal-chelating properties. This chapter is dedicated to methods used for isolation and purification, structure analysis, physicochemical properties and stability characterization, quantification, as well as in vitro and in vivo biological activities evaluation. In addition, emerging approaches related to phycobiliproteins analysis are also reviewed includi...ng interactions with other biomolecules and ions and identification of phycobiliprotein (chromo)peptides by mass spectrometry.
Кључне речи:
Allophycocyanin / Chromopeptides / Phycobiliproteins / Phycocyanin / Phycocyanobilin / PhycoerythrinИзвор:
Pigments from Microalgae Handbook, 2020, 179-201Издавач:
- Springer International Publishing
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200168 (Универзитет у Београду, Хемијски факултет) (RS-MESTD-inst-2020-200168)
- FoodEnTwin-Twinning of research activities for the frontier research in the fields of food, nutrition and environmental omics (EU-H2020-810752)
Напомена:
- Peer-reviewed manuscript: https://cherry.chem.bg.ac.rs/handle/123456789/4893
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - CHAP AU - Nikolić, Milan AU - Minić, Simeon L. AU - Mačvanin, Mirjana T. AU - Stanić-Vučinić, Dragana AU - Ćirković-Veličković, Tanja AU - Jacob-Lopes, Eduardo AU - Queiroz, Maria Isabel AU - Zepka, Leila Queiroz PY - 2020 UR - http://cherry.chem.bg.ac.rs/handle/123456789/4892 AB - The aim of this chapter is to review and discuss methodology and protocols in the analysis of phycobiliproteins (phycocyanins, allophycocyanins, and phycoerythrins) and their chromophores. Due to the presence of multiple covalently bound open-chain tetrapyrrole chromophores, phycobiliproteins are colored and strongly fluorescent molecules, with high absorption coefficients (105 to 106) and excellent fluorescent quantum yield (0.51 up to 0.98). Therefore, a vast number of methods for phycobiliproteins analysis is based on these spectral characteristics, whereas assessment of their bioactivity is related to their exceptional redox and metal-chelating properties. This chapter is dedicated to methods used for isolation and purification, structure analysis, physicochemical properties and stability characterization, quantification, as well as in vitro and in vivo biological activities evaluation. In addition, emerging approaches related to phycobiliproteins analysis are also reviewed including interactions with other biomolecules and ions and identification of phycobiliprotein (chromo)peptides by mass spectrometry. PB - Springer International Publishing T2 - Pigments from Microalgae Handbook T1 - Analytical Protocols in Phycobiliproteins Analysis SP - 179 EP - 201 DO - 10.1007/978-3-030-50971-2_8 ER -
@inbook{ author = "Nikolić, Milan and Minić, Simeon L. and Mačvanin, Mirjana T. and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja and Jacob-Lopes, Eduardo and Queiroz, Maria Isabel and Zepka, Leila Queiroz", year = "2020", abstract = "The aim of this chapter is to review and discuss methodology and protocols in the analysis of phycobiliproteins (phycocyanins, allophycocyanins, and phycoerythrins) and their chromophores. Due to the presence of multiple covalently bound open-chain tetrapyrrole chromophores, phycobiliproteins are colored and strongly fluorescent molecules, with high absorption coefficients (105 to 106) and excellent fluorescent quantum yield (0.51 up to 0.98). Therefore, a vast number of methods for phycobiliproteins analysis is based on these spectral characteristics, whereas assessment of their bioactivity is related to their exceptional redox and metal-chelating properties. This chapter is dedicated to methods used for isolation and purification, structure analysis, physicochemical properties and stability characterization, quantification, as well as in vitro and in vivo biological activities evaluation. In addition, emerging approaches related to phycobiliproteins analysis are also reviewed including interactions with other biomolecules and ions and identification of phycobiliprotein (chromo)peptides by mass spectrometry.", publisher = "Springer International Publishing", journal = "Pigments from Microalgae Handbook", booktitle = "Analytical Protocols in Phycobiliproteins Analysis", pages = "179-201", doi = "10.1007/978-3-030-50971-2_8" }
Nikolić, M., Minić, S. L., Mačvanin, M. T., Stanić-Vučinić, D., Ćirković-Veličković, T., Jacob-Lopes, E., Queiroz, M. I.,& Zepka, L. Q.. (2020). Analytical Protocols in Phycobiliproteins Analysis. in Pigments from Microalgae Handbook Springer International Publishing., 179-201. https://doi.org/10.1007/978-3-030-50971-2_8
Nikolić M, Minić SL, Mačvanin MT, Stanić-Vučinić D, Ćirković-Veličković T, Jacob-Lopes E, Queiroz MI, Zepka LQ. Analytical Protocols in Phycobiliproteins Analysis. in Pigments from Microalgae Handbook. 2020;:179-201. doi:10.1007/978-3-030-50971-2_8 .
Nikolić, Milan, Minić, Simeon L., Mačvanin, Mirjana T., Stanić-Vučinić, Dragana, Ćirković-Veličković, Tanja, Jacob-Lopes, Eduardo, Queiroz, Maria Isabel, Zepka, Leila Queiroz, "Analytical Protocols in Phycobiliproteins Analysis" in Pigments from Microalgae Handbook (2020):179-201, https://doi.org/10.1007/978-3-030-50971-2_8 . .