Interaction of SARS-CoV-2 Spike protein with phycocyanobilin
Аутори
Simović, AnaRadomirović, Mirjana Ž.
Gligorijević, Nikola
Stanić-Vučinić, Dragana
Ćirković-Veličković, Tanja
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Algae have been consumed as food and medicine for centuries. Their benefits are so pronounced, due to high concentrations of vitamins, minerals, antioxidants and proteins that they are commonly referred to as superfoods. Phycocyanobilin (PCB) is a bioactive compound of microalga Spirulina platensis. It is a blue tetrapyrrole chromophore of C-phycocyanin (C-PC), the major chromoprotein of this microalga. It is covalently attached to cysteine residues of C-PC via thioether bond. The outbreak of Coronavirus Disease 2019 (COVID-19) has posed a serious threat to global public health, calling for the development of safe and effective prophylactics and therapeutics against infection of its causative agent, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 spike (S) protein plays the most important roles in viral attachment, fusion and entry, and it serves as a target for development of antibodies, entry inhibitors and vaccines. It mediates viral entry into host cell...s by first binding to a host angiotensin-converting enzyme 2 (ACE2) receptor through the receptor-binding domain (RBD) and then fusing the viral and host membranes. This study aimed to investigate interaction of bioactive PCB with S protein and RBD respectively.
Combination of electrophoretic techniques and fluorescence spectroscopy was employed in order to examine interactions of PCB and S protein, as well as interactions of PCB and RBD, while the effects of PCB binding on RBD structure were studied by CD spectroscopy.
SDS-PAGE with Zn2+ staining has revealed that PCB covalently binds to both S protein and RBD, via free cysteine residues. Binding constants determined by fluorescence quenching method were: 2.1 x 107 M–1 for PCB and S protein, and 8.4 x 104 M–1 for PCB and RBD. Far-UV circular dichroism spectra showed that PCB influences RBD structure.
Our results support the importance of further research on covalent binding of PCB to S protein and RBD and its implications. Due to its interaction with S protein and RBD, PCB may exert one of its many bioactive effects via impact on S protein binding to ACE2 receptor.
Acknowledgments: This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No. 810752.
References:
[1] S. Minic, M. Radomirovic, N. Savkovic et al., Food Chemistry, 269(2018) 43-52.
[2] W. Tai, L. He, X. Zhang et al., Cellular & Molecular Immunology, 17(2020) 613-620.
Кључне речи:
phycocyanobilin / SARS-CoV-2 / spike proteinИзвор:
Book of Abstracts of the XXI EuroFoodChem Congress, 22-24 November 2021, On-line conference, 2021, 164-164Издавач:
- Sociedade Portuguesa de Química
Финансирање / пројекти:
- FoodEnTwin-Twinning of research activities for the frontier research in the fields of food, nutrition and environmental omics (EU-H2020-810752)
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - CONF AU - Simović, Ana AU - Radomirović, Mirjana Ž. AU - Gligorijević, Nikola AU - Stanić-Vučinić, Dragana AU - Ćirković-Veličković, Tanja PY - 2021 UR - http://cherry.chem.bg.ac.rs/handle/123456789/5132 AB - Algae have been consumed as food and medicine for centuries. Their benefits are so pronounced, due to high concentrations of vitamins, minerals, antioxidants and proteins that they are commonly referred to as superfoods. Phycocyanobilin (PCB) is a bioactive compound of microalga Spirulina platensis. It is a blue tetrapyrrole chromophore of C-phycocyanin (C-PC), the major chromoprotein of this microalga. It is covalently attached to cysteine residues of C-PC via thioether bond. The outbreak of Coronavirus Disease 2019 (COVID-19) has posed a serious threat to global public health, calling for the development of safe and effective prophylactics and therapeutics against infection of its causative agent, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 spike (S) protein plays the most important roles in viral attachment, fusion and entry, and it serves as a target for development of antibodies, entry inhibitors and vaccines. It mediates viral entry into host cells by first binding to a host angiotensin-converting enzyme 2 (ACE2) receptor through the receptor-binding domain (RBD) and then fusing the viral and host membranes. This study aimed to investigate interaction of bioactive PCB with S protein and RBD respectively. Combination of electrophoretic techniques and fluorescence spectroscopy was employed in order to examine interactions of PCB and S protein, as well as interactions of PCB and RBD, while the effects of PCB binding on RBD structure were studied by CD spectroscopy. SDS-PAGE with Zn2+ staining has revealed that PCB covalently binds to both S protein and RBD, via free cysteine residues. Binding constants determined by fluorescence quenching method were: 2.1 x 107 M–1 for PCB and S protein, and 8.4 x 104 M–1 for PCB and RBD. Far-UV circular dichroism spectra showed that PCB influences RBD structure. Our results support the importance of further research on covalent binding of PCB to S protein and RBD and its implications. Due to its interaction with S protein and RBD, PCB may exert one of its many bioactive effects via impact on S protein binding to ACE2 receptor. Acknowledgments: This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No. 810752. References: [1] S. Minic, M. Radomirovic, N. Savkovic et al., Food Chemistry, 269(2018) 43-52. [2] W. Tai, L. He, X. Zhang et al., Cellular & Molecular Immunology, 17(2020) 613-620. PB - Sociedade Portuguesa de Química C3 - Book of Abstracts of the XXI EuroFoodChem Congress, 22-24 November 2021, On-line conference T1 - Interaction of SARS-CoV-2 Spike protein with phycocyanobilin SP - 164 EP - 164 UR - https://hdl.handle.net/21.15107/rcub_cherry_5132 ER -
@conference{ author = "Simović, Ana and Radomirović, Mirjana Ž. and Gligorijević, Nikola and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja", year = "2021", abstract = "Algae have been consumed as food and medicine for centuries. Their benefits are so pronounced, due to high concentrations of vitamins, minerals, antioxidants and proteins that they are commonly referred to as superfoods. Phycocyanobilin (PCB) is a bioactive compound of microalga Spirulina platensis. It is a blue tetrapyrrole chromophore of C-phycocyanin (C-PC), the major chromoprotein of this microalga. It is covalently attached to cysteine residues of C-PC via thioether bond. The outbreak of Coronavirus Disease 2019 (COVID-19) has posed a serious threat to global public health, calling for the development of safe and effective prophylactics and therapeutics against infection of its causative agent, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 spike (S) protein plays the most important roles in viral attachment, fusion and entry, and it serves as a target for development of antibodies, entry inhibitors and vaccines. It mediates viral entry into host cells by first binding to a host angiotensin-converting enzyme 2 (ACE2) receptor through the receptor-binding domain (RBD) and then fusing the viral and host membranes. This study aimed to investigate interaction of bioactive PCB with S protein and RBD respectively. Combination of electrophoretic techniques and fluorescence spectroscopy was employed in order to examine interactions of PCB and S protein, as well as interactions of PCB and RBD, while the effects of PCB binding on RBD structure were studied by CD spectroscopy. SDS-PAGE with Zn2+ staining has revealed that PCB covalently binds to both S protein and RBD, via free cysteine residues. Binding constants determined by fluorescence quenching method were: 2.1 x 107 M–1 for PCB and S protein, and 8.4 x 104 M–1 for PCB and RBD. Far-UV circular dichroism spectra showed that PCB influences RBD structure. Our results support the importance of further research on covalent binding of PCB to S protein and RBD and its implications. Due to its interaction with S protein and RBD, PCB may exert one of its many bioactive effects via impact on S protein binding to ACE2 receptor. Acknowledgments: This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No. 810752. References: [1] S. Minic, M. Radomirovic, N. Savkovic et al., Food Chemistry, 269(2018) 43-52. [2] W. Tai, L. He, X. Zhang et al., Cellular & Molecular Immunology, 17(2020) 613-620.", publisher = "Sociedade Portuguesa de Química", journal = "Book of Abstracts of the XXI EuroFoodChem Congress, 22-24 November 2021, On-line conference", title = "Interaction of SARS-CoV-2 Spike protein with phycocyanobilin", pages = "164-164", url = "https://hdl.handle.net/21.15107/rcub_cherry_5132" }
Simović, A., Radomirović, M. Ž., Gligorijević, N., Stanić-Vučinić, D.,& Ćirković-Veličković, T.. (2021). Interaction of SARS-CoV-2 Spike protein with phycocyanobilin. in Book of Abstracts of the XXI EuroFoodChem Congress, 22-24 November 2021, On-line conference Sociedade Portuguesa de Química., 164-164. https://hdl.handle.net/21.15107/rcub_cherry_5132
Simović A, Radomirović MŽ, Gligorijević N, Stanić-Vučinić D, Ćirković-Veličković T. Interaction of SARS-CoV-2 Spike protein with phycocyanobilin. in Book of Abstracts of the XXI EuroFoodChem Congress, 22-24 November 2021, On-line conference. 2021;:164-164. https://hdl.handle.net/21.15107/rcub_cherry_5132 .
Simović, Ana, Radomirović, Mirjana Ž., Gligorijević, Nikola, Stanić-Vučinić, Dragana, Ćirković-Veličković, Tanja, "Interaction of SARS-CoV-2 Spike protein with phycocyanobilin" in Book of Abstracts of the XXI EuroFoodChem Congress, 22-24 November 2021, On-line conference (2021):164-164, https://hdl.handle.net/21.15107/rcub_cherry_5132 .