Simultaneous LC determination of tiazofurin, its acetyl and benzoyl esters and their active metabolite thiazole-4-carboxamide adenine dinucleotide in biological samples
Apstrakt
A rapid and sensitive HPLC-RP method for simultaneous determination of tiazofurin, its 5'-O acetyl and benzoyl esters and their active metabolite thiazole-4-carboxamide adenine dinucleotide was developed and validated. The method allowed determination and quantification of nanomolar quantities of these substances in cell extracts of treated cells, and was also used in kinetic studies of cellular uptake of tiazofurin and its esters from the cultivation medium. Separation of the analyzed substances from unidentified peaks from both biological materials was achieved by gradient elution, thus reducing the possibility of interference. The mobile phase consisted of a 0.1 M sodium-hydrogen phosphate, pH 5.1 and methanol. Run time was 22 min, with 5 min equilibration time. (C) 2002 Elsevier Science B.V. All rights reserved.
Ključne reči:
HPLC / tiazofarin / acetyl and benzoyl esters / TADIzvor:
Journal of Pharmaceutical and Biomedical Analysis, 2002, 30, 4, 993-999Izdavač:
- Pergamon-Elsevier Science Ltd, Oxford
DOI: 10.1016/S0731-7085(02)00448-X
ISSN: 0731-7085
PubMed: 12408890
WoS: 000179506100012
Scopus: 2-s2.0-0037038352
Kolekcije
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Tasic, J AU - Ristić, S AU - Piperski, V AU - Dacevic, M AU - Pejanovic, V AU - Jokanovic, M AU - Mandić, Ljuba M. PY - 2002 UR - https://cherry.chem.bg.ac.rs/handle/123456789/516 AB - A rapid and sensitive HPLC-RP method for simultaneous determination of tiazofurin, its 5'-O acetyl and benzoyl esters and their active metabolite thiazole-4-carboxamide adenine dinucleotide was developed and validated. The method allowed determination and quantification of nanomolar quantities of these substances in cell extracts of treated cells, and was also used in kinetic studies of cellular uptake of tiazofurin and its esters from the cultivation medium. Separation of the analyzed substances from unidentified peaks from both biological materials was achieved by gradient elution, thus reducing the possibility of interference. The mobile phase consisted of a 0.1 M sodium-hydrogen phosphate, pH 5.1 and methanol. Run time was 22 min, with 5 min equilibration time. (C) 2002 Elsevier Science B.V. All rights reserved. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Journal of Pharmaceutical and Biomedical Analysis T1 - Simultaneous LC determination of tiazofurin, its acetyl and benzoyl esters and their active metabolite thiazole-4-carboxamide adenine dinucleotide in biological samples VL - 30 IS - 4 SP - 993 EP - 999 DO - 10.1016/S0731-7085(02)00448-X ER -
@article{ author = "Tasic, J and Ristić, S and Piperski, V and Dacevic, M and Pejanovic, V and Jokanovic, M and Mandić, Ljuba M.", year = "2002", abstract = "A rapid and sensitive HPLC-RP method for simultaneous determination of tiazofurin, its 5'-O acetyl and benzoyl esters and their active metabolite thiazole-4-carboxamide adenine dinucleotide was developed and validated. The method allowed determination and quantification of nanomolar quantities of these substances in cell extracts of treated cells, and was also used in kinetic studies of cellular uptake of tiazofurin and its esters from the cultivation medium. Separation of the analyzed substances from unidentified peaks from both biological materials was achieved by gradient elution, thus reducing the possibility of interference. The mobile phase consisted of a 0.1 M sodium-hydrogen phosphate, pH 5.1 and methanol. Run time was 22 min, with 5 min equilibration time. (C) 2002 Elsevier Science B.V. All rights reserved.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Journal of Pharmaceutical and Biomedical Analysis", title = "Simultaneous LC determination of tiazofurin, its acetyl and benzoyl esters and their active metabolite thiazole-4-carboxamide adenine dinucleotide in biological samples", volume = "30", number = "4", pages = "993-999", doi = "10.1016/S0731-7085(02)00448-X" }
Tasic, J., Ristić, S., Piperski, V., Dacevic, M., Pejanovic, V., Jokanovic, M.,& Mandić, L. M.. (2002). Simultaneous LC determination of tiazofurin, its acetyl and benzoyl esters and their active metabolite thiazole-4-carboxamide adenine dinucleotide in biological samples. in Journal of Pharmaceutical and Biomedical Analysis Pergamon-Elsevier Science Ltd, Oxford., 30(4), 993-999. https://doi.org/10.1016/S0731-7085(02)00448-X
Tasic J, Ristić S, Piperski V, Dacevic M, Pejanovic V, Jokanovic M, Mandić LM. Simultaneous LC determination of tiazofurin, its acetyl and benzoyl esters and their active metabolite thiazole-4-carboxamide adenine dinucleotide in biological samples. in Journal of Pharmaceutical and Biomedical Analysis. 2002;30(4):993-999. doi:10.1016/S0731-7085(02)00448-X .
Tasic, J, Ristić, S, Piperski, V, Dacevic, M, Pejanovic, V, Jokanovic, M, Mandić, Ljuba M., "Simultaneous LC determination of tiazofurin, its acetyl and benzoyl esters and their active metabolite thiazole-4-carboxamide adenine dinucleotide in biological samples" in Journal of Pharmaceutical and Biomedical Analysis, 30, no. 4 (2002):993-999, https://doi.org/10.1016/S0731-7085(02)00448-X . .