Thin-layer chromatography in bioassays of antimicrobial compounds from plants
Abstract
Growing antibiotic resistance creates a need to find new antimicrobial agents characterized by
diverse chemical structures and pharmaceutical activity. The higher plants synthesize many specialized
metabolites as a part of their normal metabolic activity and have been extensively used for
centuries in treatment of different diseases. They have a wide activity range depending on the
species, topography and climate, and may have different categories of active principles. In contrast
to conventional antimicrobial techniques, planar chromatography in combination with biological
detection can be an appropriate method of choice for fast, simple, and low-cost screening of plant
extract for successful detection of antimicrobial agents which can be good candidates for lead
compounds. To date, all bioautography steps such as chromatographic separation, detection with
bacteria cells, incubation, and visualization of bioactive bands were improved and optimized. This
review gives an overv...iew of bioautography procedure from extraction to structure elucidation of
antimicrobial compounds from plants.
Keywords:
Antimicrobial detection / extraction / HPTLC / plant sources / structure elucidationSource:
Journal of Liquid Chromatography & Related Technologies, 2021, 44, 507-518Publisher:
- Taylor & Francis Group
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200026 (University of Belgrade, Institute of Chemistry, Technology and Metallurgy - IChTM) (RS-MESTD-inst-2020-200026)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200168 (University of Belgrade, Faculty of Chemistry) (RS-MESTD-inst-2020-200168)
DOI: 10.1080/10826076.2021.1968429
ISSN: 1520-572X
WoS: 000690315600001
Scopus: 2-s2.0-85113700242
Collections
Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Šegan, Sandra B. AU - Živković-Radovanović, Vukosava AU - Tosti, Tomislav AU - Ristivojević, Petar PY - 2021 UR - http://cherry.chem.bg.ac.rs/handle/123456789/5194 AB - Growing antibiotic resistance creates a need to find new antimicrobial agents characterized by diverse chemical structures and pharmaceutical activity. The higher plants synthesize many specialized metabolites as a part of their normal metabolic activity and have been extensively used for centuries in treatment of different diseases. They have a wide activity range depending on the species, topography and climate, and may have different categories of active principles. In contrast to conventional antimicrobial techniques, planar chromatography in combination with biological detection can be an appropriate method of choice for fast, simple, and low-cost screening of plant extract for successful detection of antimicrobial agents which can be good candidates for lead compounds. To date, all bioautography steps such as chromatographic separation, detection with bacteria cells, incubation, and visualization of bioactive bands were improved and optimized. This review gives an overview of bioautography procedure from extraction to structure elucidation of antimicrobial compounds from plants. PB - Taylor & Francis Group T2 - Journal of Liquid Chromatography & Related Technologies T1 - Thin-layer chromatography in bioassays of antimicrobial compounds from plants VL - 44 SP - 507 EP - 518 DO - 10.1080/10826076.2021.1968429 ER -
@article{ author = "Šegan, Sandra B. and Živković-Radovanović, Vukosava and Tosti, Tomislav and Ristivojević, Petar", year = "2021", abstract = "Growing antibiotic resistance creates a need to find new antimicrobial agents characterized by diverse chemical structures and pharmaceutical activity. The higher plants synthesize many specialized metabolites as a part of their normal metabolic activity and have been extensively used for centuries in treatment of different diseases. They have a wide activity range depending on the species, topography and climate, and may have different categories of active principles. In contrast to conventional antimicrobial techniques, planar chromatography in combination with biological detection can be an appropriate method of choice for fast, simple, and low-cost screening of plant extract for successful detection of antimicrobial agents which can be good candidates for lead compounds. To date, all bioautography steps such as chromatographic separation, detection with bacteria cells, incubation, and visualization of bioactive bands were improved and optimized. This review gives an overview of bioautography procedure from extraction to structure elucidation of antimicrobial compounds from plants.", publisher = "Taylor & Francis Group", journal = "Journal of Liquid Chromatography & Related Technologies", title = "Thin-layer chromatography in bioassays of antimicrobial compounds from plants", volume = "44", pages = "507-518", doi = "10.1080/10826076.2021.1968429" }
Šegan, S. B., Živković-Radovanović, V., Tosti, T.,& Ristivojević, P.. (2021). Thin-layer chromatography in bioassays of antimicrobial compounds from plants. in Journal of Liquid Chromatography & Related Technologies Taylor & Francis Group., 44, 507-518. https://doi.org/10.1080/10826076.2021.1968429
Šegan SB, Živković-Radovanović V, Tosti T, Ristivojević P. Thin-layer chromatography in bioassays of antimicrobial compounds from plants. in Journal of Liquid Chromatography & Related Technologies. 2021;44:507-518. doi:10.1080/10826076.2021.1968429 .
Šegan, Sandra B., Živković-Radovanović, Vukosava, Tosti, Tomislav, Ristivojević, Petar, "Thin-layer chromatography in bioassays of antimicrobial compounds from plants" in Journal of Liquid Chromatography & Related Technologies, 44 (2021):507-518, https://doi.org/10.1080/10826076.2021.1968429 . .