Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study
Samo za registrovane korisnike
2022
Autori
Bartolić, DraganaMojović, Miloš
Prokopijević, Miloš
Đikanović, Daniela
Kalauzi, Aleksandar
Mutavdžić, Dragosav
Baošić, Rada
Radotić, Ksenija
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Aflatoxin B1 (AFB1), is a naturally hazardous environmental contaminant, contained mainly in various cereal seeds. The maize (Zea mays L.) seeds containing various levels of the AFB1 was investigated using optical and electron paramagnetic resonance spectroscopies. The changes of lignin content and organic free radicals as indicators of the seeds' defense response to the AFB1-stressor were estimated, in the seeds' inner and outer fractions. The lignin content was determined using the Acetyl Bromide method. No correlation was found between the lignin content and the AFB1 concentration in the inner fraction (R of 0.030, p = 0.943), while a significant positive linear correlation (R of 0.9923, p= 0.00005) between the lignin content and the AFB1 concentration in the outer fraction was obtained. Employing electron paramagnetic resonance (EPR) spectroscopy, the different response of the fractions was observed, regarding the content of free radicals with increasing AFB1 concentrations. In add...ition, front-face fluorescence spectroscopy in combination with the deconvolution showed a positive linear correlation between the ratio of the green and blue spectral emission components (C4/C2) area and the AFB1 concentration in the outer fraction. Thus lignin content and the (C4/C2) area ratio may be reliable indicators for the screening of lignin changes related to the level of AFB1 in the seeds.
Ključne reči:
Maize (Zea mays L.) seeds / AFB1 / Lignin content / Free radicals / EPR spectroscopy / Fluorescence spectroscopy / food contaminantIzvor:
Journal of the Science of Food and Agriculture, 2022, 102, 6, 2500-2505Izdavač:
- Wiley
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200053 (Univerzitet u Beogradu, Institut za multidisciplinarna istraživanja) (RS-MESTD-inst-2020-200053)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200168 (Univerzitet u Beogradu, Hemijski fakultet) (RS-MESTD-inst-2020-200168)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200146 (Univerzitet u Beogradu, Fakultet za fizičku hemiju) (RS-MESTD-inst-2020-200146)
DOI: 10.1002/jsfa.11591
ISSN: 0022-5142
WoS: 000716540500001
Scopus: 2-s2.0-85118771838
Kolekcije
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Bartolić, Dragana AU - Mojović, Miloš AU - Prokopijević, Miloš AU - Đikanović, Daniela AU - Kalauzi, Aleksandar AU - Mutavdžić, Dragosav AU - Baošić, Rada AU - Radotić, Ksenija PY - 2022 UR - http://cherry.chem.bg.ac.rs/handle/123456789/5268 AB - Aflatoxin B1 (AFB1), is a naturally hazardous environmental contaminant, contained mainly in various cereal seeds. The maize (Zea mays L.) seeds containing various levels of the AFB1 was investigated using optical and electron paramagnetic resonance spectroscopies. The changes of lignin content and organic free radicals as indicators of the seeds' defense response to the AFB1-stressor were estimated, in the seeds' inner and outer fractions. The lignin content was determined using the Acetyl Bromide method. No correlation was found between the lignin content and the AFB1 concentration in the inner fraction (R of 0.030, p = 0.943), while a significant positive linear correlation (R of 0.9923, p= 0.00005) between the lignin content and the AFB1 concentration in the outer fraction was obtained. Employing electron paramagnetic resonance (EPR) spectroscopy, the different response of the fractions was observed, regarding the content of free radicals with increasing AFB1 concentrations. In addition, front-face fluorescence spectroscopy in combination with the deconvolution showed a positive linear correlation between the ratio of the green and blue spectral emission components (C4/C2) area and the AFB1 concentration in the outer fraction. Thus lignin content and the (C4/C2) area ratio may be reliable indicators for the screening of lignin changes related to the level of AFB1 in the seeds. PB - Wiley T2 - Journal of the Science of Food and Agriculture T1 - Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study VL - 102 IS - 6 SP - 2500 EP - 2505 DO - 10.1002/jsfa.11591 ER -
@article{ author = "Bartolić, Dragana and Mojović, Miloš and Prokopijević, Miloš and Đikanović, Daniela and Kalauzi, Aleksandar and Mutavdžić, Dragosav and Baošić, Rada and Radotić, Ksenija", year = "2022", abstract = "Aflatoxin B1 (AFB1), is a naturally hazardous environmental contaminant, contained mainly in various cereal seeds. The maize (Zea mays L.) seeds containing various levels of the AFB1 was investigated using optical and electron paramagnetic resonance spectroscopies. The changes of lignin content and organic free radicals as indicators of the seeds' defense response to the AFB1-stressor were estimated, in the seeds' inner and outer fractions. The lignin content was determined using the Acetyl Bromide method. No correlation was found between the lignin content and the AFB1 concentration in the inner fraction (R of 0.030, p = 0.943), while a significant positive linear correlation (R of 0.9923, p= 0.00005) between the lignin content and the AFB1 concentration in the outer fraction was obtained. Employing electron paramagnetic resonance (EPR) spectroscopy, the different response of the fractions was observed, regarding the content of free radicals with increasing AFB1 concentrations. In addition, front-face fluorescence spectroscopy in combination with the deconvolution showed a positive linear correlation between the ratio of the green and blue spectral emission components (C4/C2) area and the AFB1 concentration in the outer fraction. Thus lignin content and the (C4/C2) area ratio may be reliable indicators for the screening of lignin changes related to the level of AFB1 in the seeds.", publisher = "Wiley", journal = "Journal of the Science of Food and Agriculture", title = "Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study", volume = "102", number = "6", pages = "2500-2505", doi = "10.1002/jsfa.11591" }
Bartolić, D., Mojović, M., Prokopijević, M., Đikanović, D., Kalauzi, A., Mutavdžić, D., Baošić, R.,& Radotić, K.. (2022). Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study. in Journal of the Science of Food and Agriculture Wiley., 102(6), 2500-2505. https://doi.org/10.1002/jsfa.11591
Bartolić D, Mojović M, Prokopijević M, Đikanović D, Kalauzi A, Mutavdžić D, Baošić R, Radotić K. Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study. in Journal of the Science of Food and Agriculture. 2022;102(6):2500-2505. doi:10.1002/jsfa.11591 .
Bartolić, Dragana, Mojović, Miloš, Prokopijević, Miloš, Đikanović, Daniela, Kalauzi, Aleksandar, Mutavdžić, Dragosav, Baošić, Rada, Radotić, Ksenija, "Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study" in Journal of the Science of Food and Agriculture, 102, no. 6 (2022):2500-2505, https://doi.org/10.1002/jsfa.11591 . .