Antiproliferative activity of some cis-/trans-platinum(II) complexes on HeLa cells
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Aranđelović, SandraTešić, Živoslav Lj.
Juranić, Z.
Radulović, Siniša
Vrvić, Miroslav M.
Potkonjak, B
Ilic, Z
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Purpose of this work was to synthesize several cis-/trans- isomer pairs of the platinum(II) complexes, and study the extent and the mode of their antiproliferative activity on HeLa cells. Six platinum(II) isomer pairs have a general formula cis-/trans-[PtA(2) X-2] where A is ligand: ammonia (NH3), pyridine (Py); and X is ligand: chloride ion (Cl-), bromide ion (Br-), iodide ion (I-), thiocyanato, ion (SCN-); four compounds have different structural formulas, and these are cis-/trans-[Pt(NH2OH)(2) (NH3)(2)]Cl-2, and cis-/trans- Pt(Gly)(2), where Gly is bidentate glycinato ligand. Results of the MTT assay, showed that six cis- and one trans-platinum(II) complexes exhibited cytotoxicity (IC50) ranging between 5 and 33 muM. Most of the cis-platinum(II) isomers caused significant alteration of cell cycle phases progression, and induced apoptosis in degree that varied among different compounds, as evaluated using flowcytometry and morphological study. Spectrophotometric analysis (AAS) indica...ted that there is no correlation between intracellular platinum(II) accumulation and cytotoxicity of tested complexes.
Keywords:
cisplatin / platinum(II) complexes / MTT / apoptosis / HeLaSource:
Journal of Experimental and Clinical Cancer Research, 2002, 21, 4, 519-526Publisher:
- Bmc, London
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Aranđelović, Sandra AU - Tešić, Živoslav Lj. AU - Juranić, Z. AU - Radulović, Siniša AU - Vrvić, Miroslav M. AU - Potkonjak, B AU - Ilic, Z PY - 2002 UR - https://cherry.chem.bg.ac.rs/handle/123456789/540 AB - Purpose of this work was to synthesize several cis-/trans- isomer pairs of the platinum(II) complexes, and study the extent and the mode of their antiproliferative activity on HeLa cells. Six platinum(II) isomer pairs have a general formula cis-/trans-[PtA(2) X-2] where A is ligand: ammonia (NH3), pyridine (Py); and X is ligand: chloride ion (Cl-), bromide ion (Br-), iodide ion (I-), thiocyanato, ion (SCN-); four compounds have different structural formulas, and these are cis-/trans-[Pt(NH2OH)(2) (NH3)(2)]Cl-2, and cis-/trans- Pt(Gly)(2), where Gly is bidentate glycinato ligand. Results of the MTT assay, showed that six cis- and one trans-platinum(II) complexes exhibited cytotoxicity (IC50) ranging between 5 and 33 muM. Most of the cis-platinum(II) isomers caused significant alteration of cell cycle phases progression, and induced apoptosis in degree that varied among different compounds, as evaluated using flowcytometry and morphological study. Spectrophotometric analysis (AAS) indicated that there is no correlation between intracellular platinum(II) accumulation and cytotoxicity of tested complexes. PB - Bmc, London T2 - Journal of Experimental and Clinical Cancer Research T1 - Antiproliferative activity of some cis-/trans-platinum(II) complexes on HeLa cells VL - 21 IS - 4 SP - 519 EP - 526 UR - https://hdl.handle.net/21.15107/rcub_cherry_540 ER -
@article{ author = "Aranđelović, Sandra and Tešić, Živoslav Lj. and Juranić, Z. and Radulović, Siniša and Vrvić, Miroslav M. and Potkonjak, B and Ilic, Z", year = "2002", abstract = "Purpose of this work was to synthesize several cis-/trans- isomer pairs of the platinum(II) complexes, and study the extent and the mode of their antiproliferative activity on HeLa cells. Six platinum(II) isomer pairs have a general formula cis-/trans-[PtA(2) X-2] where A is ligand: ammonia (NH3), pyridine (Py); and X is ligand: chloride ion (Cl-), bromide ion (Br-), iodide ion (I-), thiocyanato, ion (SCN-); four compounds have different structural formulas, and these are cis-/trans-[Pt(NH2OH)(2) (NH3)(2)]Cl-2, and cis-/trans- Pt(Gly)(2), where Gly is bidentate glycinato ligand. Results of the MTT assay, showed that six cis- and one trans-platinum(II) complexes exhibited cytotoxicity (IC50) ranging between 5 and 33 muM. Most of the cis-platinum(II) isomers caused significant alteration of cell cycle phases progression, and induced apoptosis in degree that varied among different compounds, as evaluated using flowcytometry and morphological study. Spectrophotometric analysis (AAS) indicated that there is no correlation between intracellular platinum(II) accumulation and cytotoxicity of tested complexes.", publisher = "Bmc, London", journal = "Journal of Experimental and Clinical Cancer Research", title = "Antiproliferative activity of some cis-/trans-platinum(II) complexes on HeLa cells", volume = "21", number = "4", pages = "519-526", url = "https://hdl.handle.net/21.15107/rcub_cherry_540" }
Aranđelović, S., Tešić, Ž. Lj., Juranić, Z., Radulović, S., Vrvić, M. M., Potkonjak, B.,& Ilic, Z.. (2002). Antiproliferative activity of some cis-/trans-platinum(II) complexes on HeLa cells. in Journal of Experimental and Clinical Cancer Research Bmc, London., 21(4), 519-526. https://hdl.handle.net/21.15107/rcub_cherry_540
Aranđelović S, Tešić ŽL, Juranić Z, Radulović S, Vrvić MM, Potkonjak B, Ilic Z. Antiproliferative activity of some cis-/trans-platinum(II) complexes on HeLa cells. in Journal of Experimental and Clinical Cancer Research. 2002;21(4):519-526. https://hdl.handle.net/21.15107/rcub_cherry_540 .
Aranđelović, Sandra, Tešić, Živoslav Lj., Juranić, Z., Radulović, Siniša, Vrvić, Miroslav M., Potkonjak, B, Ilic, Z, "Antiproliferative activity of some cis-/trans-platinum(II) complexes on HeLa cells" in Journal of Experimental and Clinical Cancer Research, 21, no. 4 (2002):519-526, https://hdl.handle.net/21.15107/rcub_cherry_540 .