Voltammetric immunoassay based on MWCNTs@Nd(OH)3-BSA-antibody platform for sensitive BSA detection
Samo za registrovane korisnike
2022
Autori
Đurđić, Slađana Z.Ognjanović, Miloš
Krstić-Ristivojević, Maja
Antić, Bratislav
Ćirković-Veličković, Tanja
Mutić, Jelena
Kónya, Zoltán
Stanković, Dalibor
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
An electrochemical approach is presented based on multiwall carbon nanotubes (MWCNTs) and neodymium(III) hydroxide (Nd(OH)3) nanoflakes for detection of bovine serum albumin (BSA). The materials were characterized morphologically (XRPD, SEM, and HR-TEM) and electrochemically (DPV, EIS). The MWCNTs@Nd(OH)3 composite was used as support for bovine serum albumin polyclonal antibody (anti-BSA). After the antibody immobilization on the electrochemical platform and antigen/antibody binding time (optimum 60 min), the proposed approach shows a linear voltammetric response toward BSA concentration in the range 0.066 to 6.010 ng mL−1 at maximum peak potential of 0.13 V (vs. Ag/AgCl). Limit of detection (LOD) and limit of quantification (LOQ) were 18 pg mL−1 and 61 pg mL−1, respectively. The precision of the method calculated as relative standard deviation (RSD) of five independent measurements was better 3%. The selectivity of the optimized method regarding structurally similar proteins (human s...erum albumin and human hemoglobin), ions (Na+, K+, Ca2+, and NO2−), or compounds (glucose, ascorbic acid, dopamine, uric acid, paracetamol, and glycine) was found to be satisfactory, with the current changes of less than 5% in the presence of up to 1 × 105 times higher concentrations (depending on the compound) of the listed potential interfering compounds. Practical applicability of immunosensor for BSA determination in cow whey sample, with recovery values in the range 97 to 103%, shows that the developed method has high potential for precise and accurate detection of BSA, as well as exceptional miniaturization possibilities for on-site and equipment-free sensing.
Ključne reči:
bovine serum albumin / bovine serum albumin polyclonal antibody / differential pulse voltammetry / electrochemical immunosensor / multi-wall carbon nanotubes / neodymium(III) hydroxideIzvor:
Microchimica Acta, 2022, 189, 11, 422-Izdavač:
- Springer
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200168 (Univerzitet u Beogradu, Hemijski fakultet) (RS-MESTD-inst-2020-200168)
- Eureka project E! 13303 MED-BIO-TEST
Napomena:
- Supplementary material for: https://cherry.chem.bg.ac.rs/handle/123456789/5672
Povezane informacije:
Kolekcije
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Đurđić, Slađana Z. AU - Ognjanović, Miloš AU - Krstić-Ristivojević, Maja AU - Antić, Bratislav AU - Ćirković-Veličković, Tanja AU - Mutić, Jelena AU - Kónya, Zoltán AU - Stanković, Dalibor PY - 2022 UR - http://cherry.chem.bg.ac.rs/handle/123456789/5670 AB - An electrochemical approach is presented based on multiwall carbon nanotubes (MWCNTs) and neodymium(III) hydroxide (Nd(OH)3) nanoflakes for detection of bovine serum albumin (BSA). The materials were characterized morphologically (XRPD, SEM, and HR-TEM) and electrochemically (DPV, EIS). The MWCNTs@Nd(OH)3 composite was used as support for bovine serum albumin polyclonal antibody (anti-BSA). After the antibody immobilization on the electrochemical platform and antigen/antibody binding time (optimum 60 min), the proposed approach shows a linear voltammetric response toward BSA concentration in the range 0.066 to 6.010 ng mL−1 at maximum peak potential of 0.13 V (vs. Ag/AgCl). Limit of detection (LOD) and limit of quantification (LOQ) were 18 pg mL−1 and 61 pg mL−1, respectively. The precision of the method calculated as relative standard deviation (RSD) of five independent measurements was better 3%. The selectivity of the optimized method regarding structurally similar proteins (human serum albumin and human hemoglobin), ions (Na+, K+, Ca2+, and NO2−), or compounds (glucose, ascorbic acid, dopamine, uric acid, paracetamol, and glycine) was found to be satisfactory, with the current changes of less than 5% in the presence of up to 1 × 105 times higher concentrations (depending on the compound) of the listed potential interfering compounds. Practical applicability of immunosensor for BSA determination in cow whey sample, with recovery values in the range 97 to 103%, shows that the developed method has high potential for precise and accurate detection of BSA, as well as exceptional miniaturization possibilities for on-site and equipment-free sensing. PB - Springer T2 - Microchimica Acta T1 - Voltammetric immunoassay based on MWCNTs@Nd(OH)3-BSA-antibody platform for sensitive BSA detection VL - 189 IS - 11 SP - 422 DO - 10.1007/s00604-022-05514-z ER -
@article{ author = "Đurđić, Slađana Z. and Ognjanović, Miloš and Krstić-Ristivojević, Maja and Antić, Bratislav and Ćirković-Veličković, Tanja and Mutić, Jelena and Kónya, Zoltán and Stanković, Dalibor", year = "2022", abstract = "An electrochemical approach is presented based on multiwall carbon nanotubes (MWCNTs) and neodymium(III) hydroxide (Nd(OH)3) nanoflakes for detection of bovine serum albumin (BSA). The materials were characterized morphologically (XRPD, SEM, and HR-TEM) and electrochemically (DPV, EIS). The MWCNTs@Nd(OH)3 composite was used as support for bovine serum albumin polyclonal antibody (anti-BSA). After the antibody immobilization on the electrochemical platform and antigen/antibody binding time (optimum 60 min), the proposed approach shows a linear voltammetric response toward BSA concentration in the range 0.066 to 6.010 ng mL−1 at maximum peak potential of 0.13 V (vs. Ag/AgCl). Limit of detection (LOD) and limit of quantification (LOQ) were 18 pg mL−1 and 61 pg mL−1, respectively. The precision of the method calculated as relative standard deviation (RSD) of five independent measurements was better 3%. The selectivity of the optimized method regarding structurally similar proteins (human serum albumin and human hemoglobin), ions (Na+, K+, Ca2+, and NO2−), or compounds (glucose, ascorbic acid, dopamine, uric acid, paracetamol, and glycine) was found to be satisfactory, with the current changes of less than 5% in the presence of up to 1 × 105 times higher concentrations (depending on the compound) of the listed potential interfering compounds. Practical applicability of immunosensor for BSA determination in cow whey sample, with recovery values in the range 97 to 103%, shows that the developed method has high potential for precise and accurate detection of BSA, as well as exceptional miniaturization possibilities for on-site and equipment-free sensing.", publisher = "Springer", journal = "Microchimica Acta", title = "Voltammetric immunoassay based on MWCNTs@Nd(OH)3-BSA-antibody platform for sensitive BSA detection", volume = "189", number = "11", pages = "422", doi = "10.1007/s00604-022-05514-z" }
Đurđić, S. Z., Ognjanović, M., Krstić-Ristivojević, M., Antić, B., Ćirković-Veličković, T., Mutić, J., Kónya, Z.,& Stanković, D.. (2022). Voltammetric immunoassay based on MWCNTs@Nd(OH)3-BSA-antibody platform for sensitive BSA detection. in Microchimica Acta Springer., 189(11), 422. https://doi.org/10.1007/s00604-022-05514-z
Đurđić SZ, Ognjanović M, Krstić-Ristivojević M, Antić B, Ćirković-Veličković T, Mutić J, Kónya Z, Stanković D. Voltammetric immunoassay based on MWCNTs@Nd(OH)3-BSA-antibody platform for sensitive BSA detection. in Microchimica Acta. 2022;189(11):422. doi:10.1007/s00604-022-05514-z .
Đurđić, Slađana Z., Ognjanović, Miloš, Krstić-Ristivojević, Maja, Antić, Bratislav, Ćirković-Veličković, Tanja, Mutić, Jelena, Kónya, Zoltán, Stanković, Dalibor, "Voltammetric immunoassay based on MWCNTs@Nd(OH)3-BSA-antibody platform for sensitive BSA detection" in Microchimica Acta, 189, no. 11 (2022):422, https://doi.org/10.1007/s00604-022-05514-z . .