The immunoproteomics reveals novel potential allergens in ark clam seashells, besides abundant tropomyosin
Аутори
Mladenović, MajaApostolović, Danijela
Smiljanić, Katarina
Jovanović, Vesna B.
Ćirković-Veličković, Tanja
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Shellfish allergy is one of the most common food allergies with a prevalence of 0.5%-2.5% in the general population. The most common allergen present in shellfish is tropomyosin. Our aim was to probe for the presence potentially novel allergens in two blood cockles, containing hemoglobin, Anadara broughtonii (AB) and Tigellarca granosa (TG), using sera of patients allergic to seashells and shrimps. Seashells estracts were resolved by 1D and 2D-SDS-PAGE, which were in-gel trypsin digested and characterized with nLC-ESI-MS/MS. The presence of tropomyosin was confirmed by commercial tropomyosin standard, by 1D/2D blots with specific antibodies, and by mass spectrometry identification. 1D-immunoblot was used for individual patients' profiling of IgE binding, while 2D-immunoblot was used to asses IgE reactivity with the pool of patients' sera. One third of 69sera of tested patients allergic to seafood or shrimps showed cross-reactivity with blood clamps. 2D-electrophoresis showed that most ...of proteins are in acidic range with especially in the range 35-50 kDa, with tropomyosin's isoforms presence in majority of spots in both seashells. IgE reactivity of individual patients showed great pattern diversity in 1D-immunoblot. 2D-immunoblots suggests hemoglobin and arginine kinase could be novel allergens. In-depth characterization of proteins causing IgE-mediated allergies is important because it can improve diagnosis and patients' health management.
Кључне речи:
novel allergens / seashells / tropomyosin / allergy / shotgun proteomicsИзвор:
Proteomics and Metabolomics for Personalized Medicine, XV Italian Proteomics Association Annual Meeting, Catholic University of the Sacred Heart, Roma, Italy, 8th-10th September 2021, 2021, 71-71, 2021, 59-59Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200168 (Универзитет у Београду, Хемијски факултет) (RS-MESTD-inst-2020-200168)
- FoodEnTwin-Twinning of research activities for the frontier research in the fields of food, nutrition and environmental omics (EU-H2020-810752)
Напомена:
- Book of Abstracts
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - CONF AU - Mladenović, Maja AU - Apostolović, Danijela AU - Smiljanić, Katarina AU - Jovanović, Vesna B. AU - Ćirković-Veličković, Tanja PY - 2021 UR - http://cherry.chem.bg.ac.rs/handle/123456789/5734 AB - Shellfish allergy is one of the most common food allergies with a prevalence of 0.5%-2.5% in the general population. The most common allergen present in shellfish is tropomyosin. Our aim was to probe for the presence potentially novel allergens in two blood cockles, containing hemoglobin, Anadara broughtonii (AB) and Tigellarca granosa (TG), using sera of patients allergic to seashells and shrimps. Seashells estracts were resolved by 1D and 2D-SDS-PAGE, which were in-gel trypsin digested and characterized with nLC-ESI-MS/MS. The presence of tropomyosin was confirmed by commercial tropomyosin standard, by 1D/2D blots with specific antibodies, and by mass spectrometry identification. 1D-immunoblot was used for individual patients' profiling of IgE binding, while 2D-immunoblot was used to asses IgE reactivity with the pool of patients' sera. One third of 69sera of tested patients allergic to seafood or shrimps showed cross-reactivity with blood clamps. 2D-electrophoresis showed that most of proteins are in acidic range with especially in the range 35-50 kDa, with tropomyosin's isoforms presence in majority of spots in both seashells. IgE reactivity of individual patients showed great pattern diversity in 1D-immunoblot. 2D-immunoblots suggests hemoglobin and arginine kinase could be novel allergens. In-depth characterization of proteins causing IgE-mediated allergies is important because it can improve diagnosis and patients' health management. C3 - Proteomics and Metabolomics for Personalized Medicine, XV Italian Proteomics Association Annual Meeting, Catholic University of the Sacred Heart, Roma, Italy, 8th-10th September 2021, 2021, 71-71 T1 - The immunoproteomics reveals novel potential allergens in ark clam seashells, besides abundant tropomyosin SP - 59 EP - 59 UR - https://hdl.handle.net/21.15107/rcub_cherry_5734 ER -
@conference{ author = "Mladenović, Maja and Apostolović, Danijela and Smiljanić, Katarina and Jovanović, Vesna B. and Ćirković-Veličković, Tanja", year = "2021", abstract = "Shellfish allergy is one of the most common food allergies with a prevalence of 0.5%-2.5% in the general population. The most common allergen present in shellfish is tropomyosin. Our aim was to probe for the presence potentially novel allergens in two blood cockles, containing hemoglobin, Anadara broughtonii (AB) and Tigellarca granosa (TG), using sera of patients allergic to seashells and shrimps. Seashells estracts were resolved by 1D and 2D-SDS-PAGE, which were in-gel trypsin digested and characterized with nLC-ESI-MS/MS. The presence of tropomyosin was confirmed by commercial tropomyosin standard, by 1D/2D blots with specific antibodies, and by mass spectrometry identification. 1D-immunoblot was used for individual patients' profiling of IgE binding, while 2D-immunoblot was used to asses IgE reactivity with the pool of patients' sera. One third of 69sera of tested patients allergic to seafood or shrimps showed cross-reactivity with blood clamps. 2D-electrophoresis showed that most of proteins are in acidic range with especially in the range 35-50 kDa, with tropomyosin's isoforms presence in majority of spots in both seashells. IgE reactivity of individual patients showed great pattern diversity in 1D-immunoblot. 2D-immunoblots suggests hemoglobin and arginine kinase could be novel allergens. In-depth characterization of proteins causing IgE-mediated allergies is important because it can improve diagnosis and patients' health management.", journal = "Proteomics and Metabolomics for Personalized Medicine, XV Italian Proteomics Association Annual Meeting, Catholic University of the Sacred Heart, Roma, Italy, 8th-10th September 2021, 2021, 71-71", title = "The immunoproteomics reveals novel potential allergens in ark clam seashells, besides abundant tropomyosin", pages = "59-59", url = "https://hdl.handle.net/21.15107/rcub_cherry_5734" }
Mladenović, M., Apostolović, D., Smiljanić, K., Jovanović, V. B.,& Ćirković-Veličković, T.. (2021). The immunoproteomics reveals novel potential allergens in ark clam seashells, besides abundant tropomyosin. in Proteomics and Metabolomics for Personalized Medicine, XV Italian Proteomics Association Annual Meeting, Catholic University of the Sacred Heart, Roma, Italy, 8th-10th September 2021, 2021, 71-71, 59-59. https://hdl.handle.net/21.15107/rcub_cherry_5734
Mladenović M, Apostolović D, Smiljanić K, Jovanović VB, Ćirković-Veličković T. The immunoproteomics reveals novel potential allergens in ark clam seashells, besides abundant tropomyosin. in Proteomics and Metabolomics for Personalized Medicine, XV Italian Proteomics Association Annual Meeting, Catholic University of the Sacred Heart, Roma, Italy, 8th-10th September 2021, 2021, 71-71. 2021;:59-59. https://hdl.handle.net/21.15107/rcub_cherry_5734 .
Mladenović, Maja, Apostolović, Danijela, Smiljanić, Katarina, Jovanović, Vesna B., Ćirković-Veličković, Tanja, "The immunoproteomics reveals novel potential allergens in ark clam seashells, besides abundant tropomyosin" in Proteomics and Metabolomics for Personalized Medicine, XV Italian Proteomics Association Annual Meeting, Catholic University of the Sacred Heart, Roma, Italy, 8th-10th September 2021, 2021, 71-71 (2021):59-59, https://hdl.handle.net/21.15107/rcub_cherry_5734 .