Biomimetic mineralisation of periodate oxidized glucose oxidase
Аутори
Stanišić, Marija D.Popović, Nikolina
Ristić, Predrag
Balaž, Ana Marija
Senćanski, Milan
Prodanović, Radivoje
Todorović, Tamara
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Glucose oxidase (GOx) is an enzyme that belongs to a group of oxidoreductases. This enzyme catalyzes the oxidation of glucose to gluconic acid using molecular oxygen as an electron acceptor. Glucose oxidase contains carbohydrates in its structure, most often mannose and glucose (11-13%) 1. Durability of GOx in harsh conditions can be enhanced by encapsulation within metal–organic frameworks via a process called biomimetic mineralisation. We demonstrate that chemical modification of carbohydrate parts on the protein surface by periodate oxidation is an effective method for control of biomimetic mineralisation by zeolitic imidazolate framework-8 (ZIF-8). Obtained GOx-ZIF-8 biocomposite had the higher half-life at 65oC, and higher specific activity than native GOx.
Кључне речи:
glucose oxidase / biomimetic mineralisation / ZIF-8Извор:
Tenth Conference of Serbian Biochemical Society, 24 September 2021, Kragujevac, Book of Abstracts, 2021, 148-148Издавач:
- Beograd : Biohemijsko društvo Srbije
Финансирање / пројекти:
- SYMBIOSIS - Controllable Design of Efficient Enzyme"Mof Composites for Biocatalysis (RS-ScienceFundRS-Promis-6066997)
Напомена:
- Book of Abstracts
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - CONF AU - Stanišić, Marija D. AU - Popović, Nikolina AU - Ristić, Predrag AU - Balaž, Ana Marija AU - Senćanski, Milan AU - Prodanović, Radivoje AU - Todorović, Tamara PY - 2021 UR - http://cherry.chem.bg.ac.rs/handle/123456789/5748 AB - Glucose oxidase (GOx) is an enzyme that belongs to a group of oxidoreductases. This enzyme catalyzes the oxidation of glucose to gluconic acid using molecular oxygen as an electron acceptor. Glucose oxidase contains carbohydrates in its structure, most often mannose and glucose (11-13%) 1. Durability of GOx in harsh conditions can be enhanced by encapsulation within metal–organic frameworks via a process called biomimetic mineralisation. We demonstrate that chemical modification of carbohydrate parts on the protein surface by periodate oxidation is an effective method for control of biomimetic mineralisation by zeolitic imidazolate framework-8 (ZIF-8). Obtained GOx-ZIF-8 biocomposite had the higher half-life at 65oC, and higher specific activity than native GOx. PB - Beograd : Biohemijsko društvo Srbije C3 - Tenth Conference of Serbian Biochemical Society, 24 September 2021, Kragujevac, Book of Abstracts T1 - Biomimetic mineralisation of periodate oxidized glucose oxidase SP - 148 EP - 148 UR - https://hdl.handle.net/21.15107/rcub_cherry_5748 ER -
@conference{ author = "Stanišić, Marija D. and Popović, Nikolina and Ristić, Predrag and Balaž, Ana Marija and Senćanski, Milan and Prodanović, Radivoje and Todorović, Tamara", year = "2021", abstract = "Glucose oxidase (GOx) is an enzyme that belongs to a group of oxidoreductases. This enzyme catalyzes the oxidation of glucose to gluconic acid using molecular oxygen as an electron acceptor. Glucose oxidase contains carbohydrates in its structure, most often mannose and glucose (11-13%) 1. Durability of GOx in harsh conditions can be enhanced by encapsulation within metal–organic frameworks via a process called biomimetic mineralisation. We demonstrate that chemical modification of carbohydrate parts on the protein surface by periodate oxidation is an effective method for control of biomimetic mineralisation by zeolitic imidazolate framework-8 (ZIF-8). Obtained GOx-ZIF-8 biocomposite had the higher half-life at 65oC, and higher specific activity than native GOx.", publisher = "Beograd : Biohemijsko društvo Srbije", journal = "Tenth Conference of Serbian Biochemical Society, 24 September 2021, Kragujevac, Book of Abstracts", title = "Biomimetic mineralisation of periodate oxidized glucose oxidase", pages = "148-148", url = "https://hdl.handle.net/21.15107/rcub_cherry_5748" }
Stanišić, M. D., Popović, N., Ristić, P., Balaž, A. M., Senćanski, M., Prodanović, R.,& Todorović, T.. (2021). Biomimetic mineralisation of periodate oxidized glucose oxidase. in Tenth Conference of Serbian Biochemical Society, 24 September 2021, Kragujevac, Book of Abstracts Beograd : Biohemijsko društvo Srbije., 148-148. https://hdl.handle.net/21.15107/rcub_cherry_5748
Stanišić MD, Popović N, Ristić P, Balaž AM, Senćanski M, Prodanović R, Todorović T. Biomimetic mineralisation of periodate oxidized glucose oxidase. in Tenth Conference of Serbian Biochemical Society, 24 September 2021, Kragujevac, Book of Abstracts. 2021;:148-148. https://hdl.handle.net/21.15107/rcub_cherry_5748 .
Stanišić, Marija D., Popović, Nikolina, Ristić, Predrag, Balaž, Ana Marija, Senćanski, Milan, Prodanović, Radivoje, Todorović, Tamara, "Biomimetic mineralisation of periodate oxidized glucose oxidase" in Tenth Conference of Serbian Biochemical Society, 24 September 2021, Kragujevac, Book of Abstracts (2021):148-148, https://hdl.handle.net/21.15107/rcub_cherry_5748 .