Characterization of temperature-sensitive and lipopolysaccharide overproducing transposon mutants of Pseudomonas putida CA-3 affected in PHA accumulation
Само за регистроване кориснике
2009
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A library of 20 000 transposon (Tn5) mutants of the gram-negative bacterium Pseudomonas putida CA-3 was generated and screened for adverse affects in polyhydroxyalkanoates (PHA) accumulation. Two mutants of interest were characterized phenotypically. CA-3-126, a mutant disrupted in a stress-related protein Clp protease subunit ClpA, demonstrated greater decreases in PHA accumulation compared with the wild type at reduced and elevated temperatures under PHA-accumulating growth conditions. CA-3-M, which is affected in the aminotransferase class I enzyme, accumulated reduced levels of PHA relative to the wild type and had lower growth yields on all carbon sources tested. Mutant CA-3-M produced up to 10-fold higher levels of lipopolysaccharide relative to the wild type and exhibited 1.2-fold lower aminotransferase activity with phenylalanine as a substrate compared with the wild-type strain. The composition of the lipopolysaccharide produced by the mutant differed from that produced by the... wild-type strain. Growth and PHA accumulation by CA-3-M was the same as the wild type when the nitrogen concentration in the medium was increased to 265 mg N L(-1).
Кључне речи:
polyhydroxyalkanoate / lipopolysaccharide / Pseudomonas putida CA-3Извор:
FEMS Microbiology Letters / Federation of European Microbiological Societies, 2009, 292, 2, 297-305Издавач:
- Wiley-Blackwell Publishing, Inc, Malden
Финансирање / пројекти:
- Higher Education Authority, grant (PRTLI4) in the Centre for Synthesis and Chemical Biology (CSCB) at University College, Dublin.
- J.N.-R. is funded by a research grant from the Environmental Protection Agency Ireland.
- Carbohydrate Research Center is supported in part by the Department of Energy-funded (DE-FG09-93ER-20097) Center for Plant and Microbial Complex Carbohydrates.
Напомена:
- Free full text: https://doi.org/10.1111/j.1574-6968.2009.01504.x
DOI: 10.1111/j.1574-6968.2009.01504.x
ISSN: 0378-1097
PubMed: 19187205
WoS: 000263452300020
Scopus: 2-s2.0-60349127051
Колекције
Институција/група
Inovacioni centar / Innovation CentreTY - JOUR AU - Goff, Miriam AU - Nikodinović-Runić, Jasmina AU - O'Connor, Kevin E. PY - 2009 UR - https://cherry.chem.bg.ac.rs/handle/123456789/607 AB - A library of 20 000 transposon (Tn5) mutants of the gram-negative bacterium Pseudomonas putida CA-3 was generated and screened for adverse affects in polyhydroxyalkanoates (PHA) accumulation. Two mutants of interest were characterized phenotypically. CA-3-126, a mutant disrupted in a stress-related protein Clp protease subunit ClpA, demonstrated greater decreases in PHA accumulation compared with the wild type at reduced and elevated temperatures under PHA-accumulating growth conditions. CA-3-M, which is affected in the aminotransferase class I enzyme, accumulated reduced levels of PHA relative to the wild type and had lower growth yields on all carbon sources tested. Mutant CA-3-M produced up to 10-fold higher levels of lipopolysaccharide relative to the wild type and exhibited 1.2-fold lower aminotransferase activity with phenylalanine as a substrate compared with the wild-type strain. The composition of the lipopolysaccharide produced by the mutant differed from that produced by the wild-type strain. Growth and PHA accumulation by CA-3-M was the same as the wild type when the nitrogen concentration in the medium was increased to 265 mg N L(-1). PB - Wiley-Blackwell Publishing, Inc, Malden T2 - FEMS Microbiology Letters / Federation of European Microbiological Societies T1 - Characterization of temperature-sensitive and lipopolysaccharide overproducing transposon mutants of Pseudomonas putida CA-3 affected in PHA accumulation VL - 292 IS - 2 SP - 297 EP - 305 DO - 10.1111/j.1574-6968.2009.01504.x ER -
@article{ author = "Goff, Miriam and Nikodinović-Runić, Jasmina and O'Connor, Kevin E.", year = "2009", abstract = "A library of 20 000 transposon (Tn5) mutants of the gram-negative bacterium Pseudomonas putida CA-3 was generated and screened for adverse affects in polyhydroxyalkanoates (PHA) accumulation. Two mutants of interest were characterized phenotypically. CA-3-126, a mutant disrupted in a stress-related protein Clp protease subunit ClpA, demonstrated greater decreases in PHA accumulation compared with the wild type at reduced and elevated temperatures under PHA-accumulating growth conditions. CA-3-M, which is affected in the aminotransferase class I enzyme, accumulated reduced levels of PHA relative to the wild type and had lower growth yields on all carbon sources tested. Mutant CA-3-M produced up to 10-fold higher levels of lipopolysaccharide relative to the wild type and exhibited 1.2-fold lower aminotransferase activity with phenylalanine as a substrate compared with the wild-type strain. The composition of the lipopolysaccharide produced by the mutant differed from that produced by the wild-type strain. Growth and PHA accumulation by CA-3-M was the same as the wild type when the nitrogen concentration in the medium was increased to 265 mg N L(-1).", publisher = "Wiley-Blackwell Publishing, Inc, Malden", journal = "FEMS Microbiology Letters / Federation of European Microbiological Societies", title = "Characterization of temperature-sensitive and lipopolysaccharide overproducing transposon mutants of Pseudomonas putida CA-3 affected in PHA accumulation", volume = "292", number = "2", pages = "297-305", doi = "10.1111/j.1574-6968.2009.01504.x" }
Goff, M., Nikodinović-Runić, J.,& O'Connor, K. E.. (2009). Characterization of temperature-sensitive and lipopolysaccharide overproducing transposon mutants of Pseudomonas putida CA-3 affected in PHA accumulation. in FEMS Microbiology Letters / Federation of European Microbiological Societies Wiley-Blackwell Publishing, Inc, Malden., 292(2), 297-305. https://doi.org/10.1111/j.1574-6968.2009.01504.x
Goff M, Nikodinović-Runić J, O'Connor KE. Characterization of temperature-sensitive and lipopolysaccharide overproducing transposon mutants of Pseudomonas putida CA-3 affected in PHA accumulation. in FEMS Microbiology Letters / Federation of European Microbiological Societies. 2009;292(2):297-305. doi:10.1111/j.1574-6968.2009.01504.x .
Goff, Miriam, Nikodinović-Runić, Jasmina, O'Connor, Kevin E., "Characterization of temperature-sensitive and lipopolysaccharide overproducing transposon mutants of Pseudomonas putida CA-3 affected in PHA accumulation" in FEMS Microbiology Letters / Federation of European Microbiological Societies, 292, no. 2 (2009):297-305, https://doi.org/10.1111/j.1574-6968.2009.01504.x . .