Green extraction of bioactive compounds from chokeberry: screening of Natural Deep Eutectic Solvents and chemical profiling
Abstract
Natural Deep Eutectic Solvents (NADES) are new generation of ionic liquids, and promising solvents in green extraction. NADES are made by mixing two or more compounds, hydrogen bond acceptor (HBA) and hydrogen bond donor (HBD), at various molar ratios.1,2 In this paper 28 different NADES are used as eco-friendly alternatives for extraction of phenolic acids, flavonoids and anthocyanins from chokeberry with the aim of extraction efficiency screening. Twelve different compounds were used for preparation of NADES, among them choline-chloride, proline, urea, glycerol, malic acid, tartaric acid, glucose, fructose, etc. Ultrasound extraction was applied as an eco-friendly and highly-efficient technique for isolation of phenolics and solid phase extraction (SPE) was used for purification of extracts and removal of NADES compounds. Different extraction parameters were optimized: solid/ liquid ratio, volume of added water and loading volume of extracts on SPE. Repeatability of extraction proced...ure was assessed. Chemical profiles of the extracts were obtained by applying high-performance thin-layer chromatography (HPTLC) and mobile phase was optimized for simultaneously separation of anthocyanins and other phenolic compounds. Extraction efficiency of NADES were compered to acidified methanol as conventional solvents and 1% citric acid in water (w/v) which is a generally recognized as safe (GRAS) solvent. Principal component analysis (PCA) was performed to determine the similarity/dissimilarity between studied NADES and identify most important compounds (bands) for separation between them. PCA illustrate that extraction greatly depends on the compositions of eutectic mixture and shows that NADES can be “designer solvents”.
Source:
8th Conference of Young Chemists of Serbia, Belgrade, Serbia, 29th October, 2022. In: Book of Abstracts, 2022Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200168 (University of Belgrade, Faculty of Chemistry) (RS-MESTD-inst-2020-200168)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200288 (Innovation Center of the Faculty of Chemistry) (RS-MESTD-inst-2020-200288)
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Institution/Community
Inovacioni centar / Innovation CentreTY - CONF AU - Lazović, Mila Č. AU - Ristivojević, Petar AU - Trifković, Jelena AU - Milojković-Opsenica, Dušanka PY - 2022 UR - http://cherry.chem.bg.ac.rs/handle/123456789/6216 AB - Natural Deep Eutectic Solvents (NADES) are new generation of ionic liquids, and promising solvents in green extraction. NADES are made by mixing two or more compounds, hydrogen bond acceptor (HBA) and hydrogen bond donor (HBD), at various molar ratios.1,2 In this paper 28 different NADES are used as eco-friendly alternatives for extraction of phenolic acids, flavonoids and anthocyanins from chokeberry with the aim of extraction efficiency screening. Twelve different compounds were used for preparation of NADES, among them choline-chloride, proline, urea, glycerol, malic acid, tartaric acid, glucose, fructose, etc. Ultrasound extraction was applied as an eco-friendly and highly-efficient technique for isolation of phenolics and solid phase extraction (SPE) was used for purification of extracts and removal of NADES compounds. Different extraction parameters were optimized: solid/ liquid ratio, volume of added water and loading volume of extracts on SPE. Repeatability of extraction procedure was assessed. Chemical profiles of the extracts were obtained by applying high-performance thin-layer chromatography (HPTLC) and mobile phase was optimized for simultaneously separation of anthocyanins and other phenolic compounds. Extraction efficiency of NADES were compered to acidified methanol as conventional solvents and 1% citric acid in water (w/v) which is a generally recognized as safe (GRAS) solvent. Principal component analysis (PCA) was performed to determine the similarity/dissimilarity between studied NADES and identify most important compounds (bands) for separation between them. PCA illustrate that extraction greatly depends on the compositions of eutectic mixture and shows that NADES can be “designer solvents”. C3 - 8th Conference of Young Chemists of Serbia, Belgrade, Serbia, 29th October, 2022. In: Book of Abstracts T1 - Green extraction of bioactive compounds from chokeberry: screening of Natural Deep Eutectic Solvents and chemical profiling UR - https://hdl.handle.net/21.15107/rcub_cherry_6216 ER -
@conference{ author = "Lazović, Mila Č. and Ristivojević, Petar and Trifković, Jelena and Milojković-Opsenica, Dušanka", year = "2022", abstract = "Natural Deep Eutectic Solvents (NADES) are new generation of ionic liquids, and promising solvents in green extraction. NADES are made by mixing two or more compounds, hydrogen bond acceptor (HBA) and hydrogen bond donor (HBD), at various molar ratios.1,2 In this paper 28 different NADES are used as eco-friendly alternatives for extraction of phenolic acids, flavonoids and anthocyanins from chokeberry with the aim of extraction efficiency screening. Twelve different compounds were used for preparation of NADES, among them choline-chloride, proline, urea, glycerol, malic acid, tartaric acid, glucose, fructose, etc. Ultrasound extraction was applied as an eco-friendly and highly-efficient technique for isolation of phenolics and solid phase extraction (SPE) was used for purification of extracts and removal of NADES compounds. Different extraction parameters were optimized: solid/ liquid ratio, volume of added water and loading volume of extracts on SPE. Repeatability of extraction procedure was assessed. Chemical profiles of the extracts were obtained by applying high-performance thin-layer chromatography (HPTLC) and mobile phase was optimized for simultaneously separation of anthocyanins and other phenolic compounds. Extraction efficiency of NADES were compered to acidified methanol as conventional solvents and 1% citric acid in water (w/v) which is a generally recognized as safe (GRAS) solvent. Principal component analysis (PCA) was performed to determine the similarity/dissimilarity between studied NADES and identify most important compounds (bands) for separation between them. PCA illustrate that extraction greatly depends on the compositions of eutectic mixture and shows that NADES can be “designer solvents”.", journal = "8th Conference of Young Chemists of Serbia, Belgrade, Serbia, 29th October, 2022. In: Book of Abstracts", title = "Green extraction of bioactive compounds from chokeberry: screening of Natural Deep Eutectic Solvents and chemical profiling", url = "https://hdl.handle.net/21.15107/rcub_cherry_6216" }
Lazović, M. Č., Ristivojević, P., Trifković, J.,& Milojković-Opsenica, D.. (2022). Green extraction of bioactive compounds from chokeberry: screening of Natural Deep Eutectic Solvents and chemical profiling. in 8th Conference of Young Chemists of Serbia, Belgrade, Serbia, 29th October, 2022. In: Book of Abstracts. https://hdl.handle.net/21.15107/rcub_cherry_6216
Lazović MČ, Ristivojević P, Trifković J, Milojković-Opsenica D. Green extraction of bioactive compounds from chokeberry: screening of Natural Deep Eutectic Solvents and chemical profiling. in 8th Conference of Young Chemists of Serbia, Belgrade, Serbia, 29th October, 2022. In: Book of Abstracts. 2022;. https://hdl.handle.net/21.15107/rcub_cherry_6216 .
Lazović, Mila Č., Ristivojević, Petar, Trifković, Jelena , Milojković-Opsenica, Dušanka, "Green extraction of bioactive compounds from chokeberry: screening of Natural Deep Eutectic Solvents and chemical profiling" in 8th Conference of Young Chemists of Serbia, Belgrade, Serbia, 29th October, 2022. In: Book of Abstracts (2022), https://hdl.handle.net/21.15107/rcub_cherry_6216 .