Immobilization of glucoamylase via its carbohydrate moiety on macroporous poly(GMA-co-EGDMA)
Samo za registrovane korisnike
2007
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
We succeeded in the immobilization of 190 mg of periodate oxidized glucoamylase per gram of macroporous polymer. The covalently immobilized enzyme had a specific activity of 1100 U/g. The temperature and pH optimum as well as kinetic parameters were determined. The immobilized enzyme was tested in different types of reactors for hydrolysis of concentrated maltose and starch hydrolysate syrups. The DE value of 98.6, obtained the immobilized enzyme, was slightly higher than that for the soluble form, when acting on 20% substrates. During continuous use in a packed bed reactor over a period of 4 weeks the immobilized enzyme produced 1300 kg of glucose per 1 L of reactor volume without any decrease in its activity. (c) 2006 Elsevier Inc. All rights reserved.
Ključne reči:
amyloglucosidase / glycidyl methacrylate / immobilization / periodate / starchIzvor:
Enzyme and Microbial Technology, 2007, 40, 5, 1422-1426Izdavač:
- Elsevier Science Inc, New York
Finansiranje / projekti:
- Ispitivanje strukture i funkcije biološki važnih makromolekula u fiziološkim i patološkim stanjima (RS-MESTD-MPN2006-2010-142020)
DOI: 10.1016/j.enzmictec.2006.10.018
ISSN: 0141-0229
WoS: 000245519000061
Scopus: 2-s2.0-33847656715
Kolekcije
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Milosavic, N. AU - Prodanović, Radivoje AU - Jovanović, Slobodan AU - Vujčić, Zoran PY - 2007 UR - https://cherry.chem.bg.ac.rs/handle/123456789/826 AB - We succeeded in the immobilization of 190 mg of periodate oxidized glucoamylase per gram of macroporous polymer. The covalently immobilized enzyme had a specific activity of 1100 U/g. The temperature and pH optimum as well as kinetic parameters were determined. The immobilized enzyme was tested in different types of reactors for hydrolysis of concentrated maltose and starch hydrolysate syrups. The DE value of 98.6, obtained the immobilized enzyme, was slightly higher than that for the soluble form, when acting on 20% substrates. During continuous use in a packed bed reactor over a period of 4 weeks the immobilized enzyme produced 1300 kg of glucose per 1 L of reactor volume without any decrease in its activity. (c) 2006 Elsevier Inc. All rights reserved. PB - Elsevier Science Inc, New York T2 - Enzyme and Microbial Technology T1 - Immobilization of glucoamylase via its carbohydrate moiety on macroporous poly(GMA-co-EGDMA) VL - 40 IS - 5 SP - 1422 EP - 1426 DO - 10.1016/j.enzmictec.2006.10.018 ER -
@article{ author = "Milosavic, N. and Prodanović, Radivoje and Jovanović, Slobodan and Vujčić, Zoran", year = "2007", abstract = "We succeeded in the immobilization of 190 mg of periodate oxidized glucoamylase per gram of macroporous polymer. The covalently immobilized enzyme had a specific activity of 1100 U/g. The temperature and pH optimum as well as kinetic parameters were determined. The immobilized enzyme was tested in different types of reactors for hydrolysis of concentrated maltose and starch hydrolysate syrups. The DE value of 98.6, obtained the immobilized enzyme, was slightly higher than that for the soluble form, when acting on 20% substrates. During continuous use in a packed bed reactor over a period of 4 weeks the immobilized enzyme produced 1300 kg of glucose per 1 L of reactor volume without any decrease in its activity. (c) 2006 Elsevier Inc. All rights reserved.", publisher = "Elsevier Science Inc, New York", journal = "Enzyme and Microbial Technology", title = "Immobilization of glucoamylase via its carbohydrate moiety on macroporous poly(GMA-co-EGDMA)", volume = "40", number = "5", pages = "1422-1426", doi = "10.1016/j.enzmictec.2006.10.018" }
Milosavic, N., Prodanović, R., Jovanović, S.,& Vujčić, Z.. (2007). Immobilization of glucoamylase via its carbohydrate moiety on macroporous poly(GMA-co-EGDMA). in Enzyme and Microbial Technology Elsevier Science Inc, New York., 40(5), 1422-1426. https://doi.org/10.1016/j.enzmictec.2006.10.018
Milosavic N, Prodanović R, Jovanović S, Vujčić Z. Immobilization of glucoamylase via its carbohydrate moiety on macroporous poly(GMA-co-EGDMA). in Enzyme and Microbial Technology. 2007;40(5):1422-1426. doi:10.1016/j.enzmictec.2006.10.018 .
Milosavic, N., Prodanović, Radivoje, Jovanović, Slobodan, Vujčić, Zoran, "Immobilization of glucoamylase via its carbohydrate moiety on macroporous poly(GMA-co-EGDMA)" in Enzyme and Microbial Technology, 40, no. 5 (2007):1422-1426, https://doi.org/10.1016/j.enzmictec.2006.10.018 . .