The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate
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2008
Authors
Nikodinović-Runić, JasminaKenny, Shane T.
Babu, Ramesh P.
Woods, Trevor
Blau, Werner J.
O'Connor, Kevin E.
Article (Published version)
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Here, we report the use of petrochemical aromatic hydrocarbons as a feedstock for the biotechnological conversion into valuable biodegradable plastic polymers-polyhydroxyalkanoates (PHAs). We assessed the ability of the known Pseudomonas putida species that are able to utilize benzene, toluene, ethylbenzene, p-xylene (BTEX) compounds as a sole carbon and energy source for their ability to produce PHA from the single substrates. P. putida F1 is able to accumulate medium-chain-length (mcl) PHA when supplied with toluene, benzene, or ethylbenzene. P. putida mt-2 accumulates mcl-PHA when supplied with toluene or p-xylene. The highest level of PHA accumulated by cultures in shake flask was 26% cell dry weight for P. putida mt-2 supplied with p-xylene. A synthetic mixture of benzene, toluene, ethylbenzene, p-xylene, and styrene (BTEXS) which mimics the aromatic fraction of mixed plastic pyrolysis oil was supplied to a defined mixed culture of P. putida F1, mt-2, and CA-3 in the shake flasks ...and fermentation experiments. PHA was accumulated to 24% and to 36% of the cell dry weight of the shake flask and fermentation grown cultures respectively. In addition a three-fold higher cell density was achieved with the mixed culture grown in the bioreactor compared to shake flask experiments. A run in the 5-l fermentor resulted in the utilization of 59.6 g (67.5 ml) of the BTEXS mixture and the production of 6 g of mcl-PHA. The monomer composition of PHA accumulated by the mixed culture was the same as that accumulated by single strains supplied with single substrates with 3-hydroxydecanoic acid occurring as the predominant monomer. The purified polymer was partially crystalline with an average molecular weight of 86.9 kDa. It has a thermal degradation temperature of 350 degrees C and a glass transition temperature of -48.5 degrees C.
Source:
Applied Microbiology and Biotechnology, 2008, 80, 4, 665-673Publisher:
- Springer, New York
DOI: 10.1007/s00253-008-1593-0
ISSN: 0175-7598
PubMed: 18629491
WoS: 000258881400013
Scopus: 2-s2.0-50849084452
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Inovacioni centar / Innovation CentreTY - JOUR AU - Nikodinović-Runić, Jasmina AU - Kenny, Shane T. AU - Babu, Ramesh P. AU - Woods, Trevor AU - Blau, Werner J. AU - O'Connor, Kevin E. PY - 2008 UR - https://cherry.chem.bg.ac.rs/handle/123456789/967 AB - Here, we report the use of petrochemical aromatic hydrocarbons as a feedstock for the biotechnological conversion into valuable biodegradable plastic polymers-polyhydroxyalkanoates (PHAs). We assessed the ability of the known Pseudomonas putida species that are able to utilize benzene, toluene, ethylbenzene, p-xylene (BTEX) compounds as a sole carbon and energy source for their ability to produce PHA from the single substrates. P. putida F1 is able to accumulate medium-chain-length (mcl) PHA when supplied with toluene, benzene, or ethylbenzene. P. putida mt-2 accumulates mcl-PHA when supplied with toluene or p-xylene. The highest level of PHA accumulated by cultures in shake flask was 26% cell dry weight for P. putida mt-2 supplied with p-xylene. A synthetic mixture of benzene, toluene, ethylbenzene, p-xylene, and styrene (BTEXS) which mimics the aromatic fraction of mixed plastic pyrolysis oil was supplied to a defined mixed culture of P. putida F1, mt-2, and CA-3 in the shake flasks and fermentation experiments. PHA was accumulated to 24% and to 36% of the cell dry weight of the shake flask and fermentation grown cultures respectively. In addition a three-fold higher cell density was achieved with the mixed culture grown in the bioreactor compared to shake flask experiments. A run in the 5-l fermentor resulted in the utilization of 59.6 g (67.5 ml) of the BTEXS mixture and the production of 6 g of mcl-PHA. The monomer composition of PHA accumulated by the mixed culture was the same as that accumulated by single strains supplied with single substrates with 3-hydroxydecanoic acid occurring as the predominant monomer. The purified polymer was partially crystalline with an average molecular weight of 86.9 kDa. It has a thermal degradation temperature of 350 degrees C and a glass transition temperature of -48.5 degrees C. PB - Springer, New York T2 - Applied Microbiology and Biotechnology T1 - The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate VL - 80 IS - 4 SP - 665 EP - 673 DO - 10.1007/s00253-008-1593-0 ER -
@article{ author = "Nikodinović-Runić, Jasmina and Kenny, Shane T. and Babu, Ramesh P. and Woods, Trevor and Blau, Werner J. and O'Connor, Kevin E.", year = "2008", abstract = "Here, we report the use of petrochemical aromatic hydrocarbons as a feedstock for the biotechnological conversion into valuable biodegradable plastic polymers-polyhydroxyalkanoates (PHAs). We assessed the ability of the known Pseudomonas putida species that are able to utilize benzene, toluene, ethylbenzene, p-xylene (BTEX) compounds as a sole carbon and energy source for their ability to produce PHA from the single substrates. P. putida F1 is able to accumulate medium-chain-length (mcl) PHA when supplied with toluene, benzene, or ethylbenzene. P. putida mt-2 accumulates mcl-PHA when supplied with toluene or p-xylene. The highest level of PHA accumulated by cultures in shake flask was 26% cell dry weight for P. putida mt-2 supplied with p-xylene. A synthetic mixture of benzene, toluene, ethylbenzene, p-xylene, and styrene (BTEXS) which mimics the aromatic fraction of mixed plastic pyrolysis oil was supplied to a defined mixed culture of P. putida F1, mt-2, and CA-3 in the shake flasks and fermentation experiments. PHA was accumulated to 24% and to 36% of the cell dry weight of the shake flask and fermentation grown cultures respectively. In addition a three-fold higher cell density was achieved with the mixed culture grown in the bioreactor compared to shake flask experiments. A run in the 5-l fermentor resulted in the utilization of 59.6 g (67.5 ml) of the BTEXS mixture and the production of 6 g of mcl-PHA. The monomer composition of PHA accumulated by the mixed culture was the same as that accumulated by single strains supplied with single substrates with 3-hydroxydecanoic acid occurring as the predominant monomer. The purified polymer was partially crystalline with an average molecular weight of 86.9 kDa. It has a thermal degradation temperature of 350 degrees C and a glass transition temperature of -48.5 degrees C.", publisher = "Springer, New York", journal = "Applied Microbiology and Biotechnology", title = "The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate", volume = "80", number = "4", pages = "665-673", doi = "10.1007/s00253-008-1593-0" }
Nikodinović-Runić, J., Kenny, S. T., Babu, R. P., Woods, T., Blau, W. J.,& O'Connor, K. E.. (2008). The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate. in Applied Microbiology and Biotechnology Springer, New York., 80(4), 665-673. https://doi.org/10.1007/s00253-008-1593-0
Nikodinović-Runić J, Kenny ST, Babu RP, Woods T, Blau WJ, O'Connor KE. The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate. in Applied Microbiology and Biotechnology. 2008;80(4):665-673. doi:10.1007/s00253-008-1593-0 .
Nikodinović-Runić, Jasmina, Kenny, Shane T., Babu, Ramesh P., Woods, Trevor, Blau, Werner J., O'Connor, Kevin E., "The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate" in Applied Microbiology and Biotechnology, 80, no. 4 (2008):665-673, https://doi.org/10.1007/s00253-008-1593-0 . .