TY  - JOUR
AU  - Descamps, Julie
AU  - Zhao, Yiran
AU  - Goudeau, Bertrand
AU  - Manojlović, Dragan D.
AU  - Loget, Gabriel
AU  - Šojić, Nešo
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6441
AB  - Electrochemiluminescence (ECL) is evolving rapidly from a purely analytical technique into a powerful microscopy. Herein, we report the imaging of single cells by photoinduced ECL (PECL; λem = 620 nm) stimulated by an incident near-infrared light (λexc = 1050 nm). The cells were grown on a metal–insulator–semiconductor (MIS) n-Si/SiOx/Ir photoanode that exhibited stable and bright PECL emission. The large anti-Stokes shift allowed for the recording of well-resolved images of cells with high sensitivity. PECL microscopy is demonstrated at a remarkably low onset potential of 0.8 V; this contrasts with classic ECL, which is blind at this potential. Two imaging modes are reported: (i) photoinduced positive ECL (PECL+), showing the cell membranes labeled with the [Ru(bpy)3]2+ complex; and (ii) photoinduced shadow label-free ECL (PECL−) of cell morphology, with the luminophore in the solution. Finally, by adding a new dimension with the near-infrared light stimulus, PECL microscopy should find promising applications to image and study single photoactive nanoparticles and biological entities.
PB  - Royal Society of Chemistry
T2  - Chemical Science
T1  - Infrared photoinduced electrochemiluminescence microscopy of single cells
VL  - 15
IS  - 6
SP  - 2055
EP  - 2061
DO  - 10.1039/D3SC05983A
ER  - 

@article{
author = "Descamps, Julie and Zhao, Yiran and Goudeau, Bertrand and Manojlović, Dragan D. and Loget, Gabriel and Šojić, Nešo",
year = "2024",
abstract = "Electrochemiluminescence (ECL) is evolving rapidly from a purely analytical technique into a powerful microscopy. Herein, we report the imaging of single cells by photoinduced ECL (PECL; λem = 620 nm) stimulated by an incident near-infrared light (λexc = 1050 nm). The cells were grown on a metal–insulator–semiconductor (MIS) n-Si/SiOx/Ir photoanode that exhibited stable and bright PECL emission. The large anti-Stokes shift allowed for the recording of well-resolved images of cells with high sensitivity. PECL microscopy is demonstrated at a remarkably low onset potential of 0.8 V; this contrasts with classic ECL, which is blind at this potential. Two imaging modes are reported: (i) photoinduced positive ECL (PECL+), showing the cell membranes labeled with the [Ru(bpy)3]2+ complex; and (ii) photoinduced shadow label-free ECL (PECL−) of cell morphology, with the luminophore in the solution. Finally, by adding a new dimension with the near-infrared light stimulus, PECL microscopy should find promising applications to image and study single photoactive nanoparticles and biological entities.",
publisher = "Royal Society of Chemistry",
journal = "Chemical Science",
title = "Infrared photoinduced electrochemiluminescence microscopy of single cells",
volume = "15",
number = "6",
pages = "2055-2061",
doi = "10.1039/D3SC05983A"
}

Descamps, J., Zhao, Y., Goudeau, B., Manojlović, D. D., Loget, G.,& Šojić, N.. (2024). Infrared photoinduced electrochemiluminescence microscopy of single cells. in Chemical Science
Royal Society of Chemistry., 15(6), 2055-2061.
https://doi.org/10.1039/D3SC05983A

Descamps J, Zhao Y, Goudeau B, Manojlović DD, Loget G, Šojić N. Infrared photoinduced electrochemiluminescence microscopy of single cells. in Chemical Science. 2024;15(6):2055-2061.
doi:10.1039/D3SC05983A .

Descamps, Julie, Zhao, Yiran, Goudeau, Bertrand, Manojlović, Dragan D., Loget, Gabriel, Šojić, Nešo, "Infrared photoinduced electrochemiluminescence microscopy of single cells" in Chemical Science, 15, no. 6 (2024):2055-2061,
https://doi.org/10.1039/D3SC05983A . .