TY  - JOUR
AU  - Rašković, Brankica
AU  - Bozovic, Olga
AU  - Prodanović, Radivoje
AU  - Niketić, Vesna
AU  - Polović, Natalija
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1902
AB  - A novel collagenolytic serine protease was identified and then purified (along with ficin) to apparent homogeneity from the latex of fig (Ficus carica, var. Brown Turkey) by two step chromatographic procedure using gel and covalent chromatography. The enzyme is a monomeric protein of molecular mass of 41 +/- 9 kDa as estimated by analytical gel filtration chromatography. It is an acidic protein with a pI value of approximately 5 and optimal activity at pH 8.0-8.5 and temperature 60 degrees C. The enzymatic activity was strongly inhibited by PMSF and Pefabloc SC, indicating that the enzyme is a serine protease. The enzyme showed specificity towards gelatin and collagen (215 GDU/mg and 24.8 CDU/mg, respectively) and non-specific protease activity (0.18 U/mg against casein). The enzyme was stable and retained full activity over a broad range of pH and temperature. The fig latex collagenolytic protease is potentially useful as a non-microbial enzyme with collagenolytic activity for various applications in the fields of biochemistry, biotechnology and medicine. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.
PB  - Soc Bioscience Bioengineering Japan, Osaka
T2  - Journal of Bioscience and Bioengineering
T1  - Identification, purification and characterization of a novel collagenolytic serine protease from fig (Ficus carica var. Brown Turkey) latex
VL  - 118
IS  - 6
SP  - 622
EP  - 627
DO  - 10.1016/j.jbiosc.2014.05.020
ER  - 

@article{
author = "Rašković, Brankica and Bozovic, Olga and Prodanović, Radivoje and Niketić, Vesna and Polović, Natalija",
year = "2014",
abstract = "A novel collagenolytic serine protease was identified and then purified (along with ficin) to apparent homogeneity from the latex of fig (Ficus carica, var. Brown Turkey) by two step chromatographic procedure using gel and covalent chromatography. The enzyme is a monomeric protein of molecular mass of 41 +/- 9 kDa as estimated by analytical gel filtration chromatography. It is an acidic protein with a pI value of approximately 5 and optimal activity at pH 8.0-8.5 and temperature 60 degrees C. The enzymatic activity was strongly inhibited by PMSF and Pefabloc SC, indicating that the enzyme is a serine protease. The enzyme showed specificity towards gelatin and collagen (215 GDU/mg and 24.8 CDU/mg, respectively) and non-specific protease activity (0.18 U/mg against casein). The enzyme was stable and retained full activity over a broad range of pH and temperature. The fig latex collagenolytic protease is potentially useful as a non-microbial enzyme with collagenolytic activity for various applications in the fields of biochemistry, biotechnology and medicine. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.",
publisher = "Soc Bioscience Bioengineering Japan, Osaka",
journal = "Journal of Bioscience and Bioengineering",
title = "Identification, purification and characterization of a novel collagenolytic serine protease from fig (Ficus carica var. Brown Turkey) latex",
volume = "118",
number = "6",
pages = "622-627",
doi = "10.1016/j.jbiosc.2014.05.020"
}

Rašković, B., Bozovic, O., Prodanović, R., Niketić, V.,& Polović, N.. (2014). Identification, purification and characterization of a novel collagenolytic serine protease from fig (Ficus carica var. Brown Turkey) latex. in Journal of Bioscience and Bioengineering
Soc Bioscience Bioengineering Japan, Osaka., 118(6), 622-627.
https://doi.org/10.1016/j.jbiosc.2014.05.020

Rašković B, Bozovic O, Prodanović R, Niketić V, Polović N. Identification, purification and characterization of a novel collagenolytic serine protease from fig (Ficus carica var. Brown Turkey) latex. in Journal of Bioscience and Bioengineering. 2014;118(6):622-627.
doi:10.1016/j.jbiosc.2014.05.020 .

Rašković, Brankica, Bozovic, Olga, Prodanović, Radivoje, Niketić, Vesna, Polović, Natalija, "Identification, purification and characterization of a novel collagenolytic serine protease from fig (Ficus carica var. Brown Turkey) latex" in Journal of Bioscience and Bioengineering, 118, no. 6 (2014):622-627,
https://doi.org/10.1016/j.jbiosc.2014.05.020 . .