TY  - JOUR
AU  - Ognjenović, Jana
AU  - Stojadinović, Marija M.
AU  - Milčić, Miloš K.
AU  - Apostolović, Danijela
AU  - Mihailović-Vesić, Jelena
AU  - Stambolić, Ivan
AU  - Atanasković-Marković, Marina
AU  - Simonovic, Miljan
AU  - Ćirković-Veličković, Tanja
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1820
AB  - Polyphenols, the potent plant secondary metabolites, have beneficial effects on human health, but the mechanism(s) by which these effects are exerted is not well understood. Here, we present the detailed analysis of the interactions between the major green tea catechin, epigallo-catechin 3-gallate (EGCG), and the major dietary protein and allergen, ovalbumin (OVA). We show that EGCG binds to the pocket that partly overlaps with the previously identified IgE-binding region in OVA, and that this interaction induces structural changes in the allergen. Moreover, our ex vivo studies reveal that OVA binds IgE and stimulates degranulation of basophils, and that its uptake by monocytes proceeds at a slower rate in the presence of EGCG. This study provides further evidence in support of the proposed mechanism by which EGCG interactions with the food allergens contribute to its diverse biological activities and may impair antigen uptake by antigen-presenting cells. (C) 2014 Elsevier Ltd. All rights reserved.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Interactions of epigallo-catechin 3-gallate and ovalbumin, the major allergen of egg white
VL  - 164
SP  - 36
EP  - 43
DO  - 10.1016/j.foodchem.2014.05.005
ER  - 

@article{
author = "Ognjenović, Jana and Stojadinović, Marija M. and Milčić, Miloš K. and Apostolović, Danijela and Mihailović-Vesić, Jelena and Stambolić, Ivan and Atanasković-Marković, Marina and Simonovic, Miljan and Ćirković-Veličković, Tanja",
year = "2014",
abstract = "Polyphenols, the potent plant secondary metabolites, have beneficial effects on human health, but the mechanism(s) by which these effects are exerted is not well understood. Here, we present the detailed analysis of the interactions between the major green tea catechin, epigallo-catechin 3-gallate (EGCG), and the major dietary protein and allergen, ovalbumin (OVA). We show that EGCG binds to the pocket that partly overlaps with the previously identified IgE-binding region in OVA, and that this interaction induces structural changes in the allergen. Moreover, our ex vivo studies reveal that OVA binds IgE and stimulates degranulation of basophils, and that its uptake by monocytes proceeds at a slower rate in the presence of EGCG. This study provides further evidence in support of the proposed mechanism by which EGCG interactions with the food allergens contribute to its diverse biological activities and may impair antigen uptake by antigen-presenting cells. (C) 2014 Elsevier Ltd. All rights reserved.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Interactions of epigallo-catechin 3-gallate and ovalbumin, the major allergen of egg white",
volume = "164",
pages = "36-43",
doi = "10.1016/j.foodchem.2014.05.005"
}

Ognjenović, J., Stojadinović, M. M., Milčić, M. K., Apostolović, D., Mihailović-Vesić, J., Stambolić, I., Atanasković-Marković, M., Simonovic, M.,& Ćirković-Veličković, T.. (2014). Interactions of epigallo-catechin 3-gallate and ovalbumin, the major allergen of egg white. in Food Chemistry
Elsevier Sci Ltd, Oxford., 164, 36-43.
https://doi.org/10.1016/j.foodchem.2014.05.005

Ognjenović J, Stojadinović MM, Milčić MK, Apostolović D, Mihailović-Vesić J, Stambolić I, Atanasković-Marković M, Simonovic M, Ćirković-Veličković T. Interactions of epigallo-catechin 3-gallate and ovalbumin, the major allergen of egg white. in Food Chemistry. 2014;164:36-43.
doi:10.1016/j.foodchem.2014.05.005 .

Ognjenović, Jana, Stojadinović, Marija M., Milčić, Miloš K., Apostolović, Danijela, Mihailović-Vesić, Jelena, Stambolić, Ivan, Atanasković-Marković, Marina, Simonovic, Miljan, Ćirković-Veličković, Tanja, "Interactions of epigallo-catechin 3-gallate and ovalbumin, the major allergen of egg white" in Food Chemistry, 164 (2014):36-43,
https://doi.org/10.1016/j.foodchem.2014.05.005 . .

TY  - THES
AU  - Ognjenović, Jana
PY  - 2013
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=1164
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:8019/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=45088271
UR  - http://nardus.mpn.gov.rs/123456789/3496
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2648
AB  - Učestalost pojave alergijskog sindroma u poslednjih par decenija je značajnouvećana, te se alergije i alergijska oboljenja danas često nazivaju „bolest 21. veka“.Prema trenutnim podacima, oko 25% svetske populacije pati od nekog oblika alergije,dok u ekonomski razvijenim zemljama taj procenat seže i do 40%. Postoji nekolikohipoteza koje pokušavaju da objasne ovaj trend porasta učestalosti alergijske reakcije,ali uzrok još uvek nije poznat.Alergijska reakcija se javlja kada IgE antitela alergične osobe prepoznajuspecifične proteine iz polena, tzv. alergene. Poleni drveća, trava i korovskih biljakasmatraju se jednim od najčešćih izvora alergena, te samim tim i izazivačima alergijskereakcije. Alergeni potencijal specifičnog polena ne zavisi samo od alergične osobe, veći od godišnjeg doba i geografskog regiona. Tako, za predeo severne Evropekarakteristična je alergija na polen breze (Betula verrucosa), dok je u predelu južneEvrope i Mediterana prisutna alergija na polen masline (Olea europea). Međutim, poredovih poznatih izvora alergena, nije izučeno da li poleni drugih široko raspostranjenihbiljnih vrsta izazivaju alergijske reakcije. U ovoj doktorskoj disertaciji je izučavanalergijski odgovor na polen korišćenjem dva model sistema. U prvom je izučavanaalergija humane populacije na polen lipe, dok je u drugom izučavan alergijski odgovorpopulacije kućnih pasa na polen kratke ambrozije.U ovoj doktorskoj disertaciji je, po našem saznanju, po prvi put urađenakarakterizacija alergenog potencijala polena lipe u humanoj populaciji. Takođe, u ovomradu prikazana je analiza ukrštene reaktivnosti polena lipe sa polenima masline i breze uhumanim populacijama iz različitih geografskih i klimatskih regiona. Naši rezultatiukazuju da je lipa potencijalni izvor alergena, da postoji visok stepen ukrštenereaktivnosti sa polenima drugih vrsta drveća i da je potrebno uvrstiti ovaj polen ukliničku paletu polena koja se koristi u alergološkoj dijagnostici. Takođe, u ovojdisertaciji potvrđeno je da polen kratke ambrozije u Srbiji uzrokuje atopični dermatitiskod populacije kućnih pasa, određeni su njegovi glavni alergeni izazivači, urađena je uporedna analiza profila alergijskih reakcija pasa i humanih pacijenata na polenambrozije, i ustanovljene sličnosti i razlike u karakteru i identitetu glavnih alergena...
AB  - The prevalence of allergic reactions has increased significantly in the last fewdecades, which prompted the scientific community to name allergies and allergicdisorders as “the disease of the 21st century”. According to the current data,approximately 25% of the world population suffers from some form of allergy, whereasthis number approaches 40% in the Western industrialized countries. Severalhypotheses had been put forward in an attempt to explain the basis for the increase inthe allergy prevalence, yet the cause remains unknown.An allergic reaction appears when the IgE antibodies of the allergic individualrecognize specific proteins from the pollen, which are called allergens. Perhaps the mostfrequent sources of allergens and causes of allergies are the pollens from trees, grassesand weeds. The allergenic potential of the particular pollen depends not only on theallergic individual, but also on the season and geographic region. For instance, allergyagainst the birch pollen (Betula verrucosa) is present in the Northern Europe, whereasthe allergic reaction to the olive tree pollen (Olea europea) is widespread in theSouthern Europe and Mediterranean. However, besides the well-known sources ofallergens, it is not clear if pollens from other plant species that are well distributedacross the continent are capable of eliciting the allergic response. In this doctoral thesis,the allergic response to pollen was studied by using two model systems. In one, theallergic response of human patients against the linden pollen was characterized, while inthe other, the canine allergy in Serbia against the pollen from short ragweed was studied.Herein, a detailed characterization of the allergenic potential of the linden pollenamong human patients is presented for the first time. Also, results supporting the crossreactivitybetween the pollens from the linden, birch, and olive trees in patients fromdistinct climate and geographic regions in Europe are shown. The findings suggest thatthe linden tree is a potential source of allergens, that there is a significant level of crossreactivitybetween the linden tree pollen and the pollens from other tree species, andthat it is advisable to incorporate the linden pollen into the clinical pallete of allergens...
PB  - Универзитет у Београду, Хемијски факултет
T2  - Универзитет у Београду
T1  - Imunološka karakterizacija polena lipa i modulacija imunskog odgovora prirodnim aromatičnim jedinjenjima
T1  - Immunological characterization of the linden pollens and modulation of the immune response by naturally occurring aromatic compounds
UR  - https://hdl.handle.net/21.15107/rcub_nardus_3496
ER  - 

@phdthesis{
author = "Ognjenović, Jana",
year = "2013",
abstract = "Učestalost pojave alergijskog sindroma u poslednjih par decenija je značajnouvećana, te se alergije i alergijska oboljenja danas često nazivaju „bolest 21. veka“.Prema trenutnim podacima, oko 25% svetske populacije pati od nekog oblika alergije,dok u ekonomski razvijenim zemljama taj procenat seže i do 40%. Postoji nekolikohipoteza koje pokušavaju da objasne ovaj trend porasta učestalosti alergijske reakcije,ali uzrok još uvek nije poznat.Alergijska reakcija se javlja kada IgE antitela alergične osobe prepoznajuspecifične proteine iz polena, tzv. alergene. Poleni drveća, trava i korovskih biljakasmatraju se jednim od najčešćih izvora alergena, te samim tim i izazivačima alergijskereakcije. Alergeni potencijal specifičnog polena ne zavisi samo od alergične osobe, veći od godišnjeg doba i geografskog regiona. Tako, za predeo severne Evropekarakteristična je alergija na polen breze (Betula verrucosa), dok je u predelu južneEvrope i Mediterana prisutna alergija na polen masline (Olea europea). Međutim, poredovih poznatih izvora alergena, nije izučeno da li poleni drugih široko raspostranjenihbiljnih vrsta izazivaju alergijske reakcije. U ovoj doktorskoj disertaciji je izučavanalergijski odgovor na polen korišćenjem dva model sistema. U prvom je izučavanaalergija humane populacije na polen lipe, dok je u drugom izučavan alergijski odgovorpopulacije kućnih pasa na polen kratke ambrozije.U ovoj doktorskoj disertaciji je, po našem saznanju, po prvi put urađenakarakterizacija alergenog potencijala polena lipe u humanoj populaciji. Takođe, u ovomradu prikazana je analiza ukrštene reaktivnosti polena lipe sa polenima masline i breze uhumanim populacijama iz različitih geografskih i klimatskih regiona. Naši rezultatiukazuju da je lipa potencijalni izvor alergena, da postoji visok stepen ukrštenereaktivnosti sa polenima drugih vrsta drveća i da je potrebno uvrstiti ovaj polen ukliničku paletu polena koja se koristi u alergološkoj dijagnostici. Takođe, u ovojdisertaciji potvrđeno je da polen kratke ambrozije u Srbiji uzrokuje atopični dermatitiskod populacije kućnih pasa, određeni su njegovi glavni alergeni izazivači, urađena je uporedna analiza profila alergijskih reakcija pasa i humanih pacijenata na polenambrozije, i ustanovljene sličnosti i razlike u karakteru i identitetu glavnih alergena..., The prevalence of allergic reactions has increased significantly in the last fewdecades, which prompted the scientific community to name allergies and allergicdisorders as “the disease of the 21st century”. According to the current data,approximately 25% of the world population suffers from some form of allergy, whereasthis number approaches 40% in the Western industrialized countries. Severalhypotheses had been put forward in an attempt to explain the basis for the increase inthe allergy prevalence, yet the cause remains unknown.An allergic reaction appears when the IgE antibodies of the allergic individualrecognize specific proteins from the pollen, which are called allergens. Perhaps the mostfrequent sources of allergens and causes of allergies are the pollens from trees, grassesand weeds. The allergenic potential of the particular pollen depends not only on theallergic individual, but also on the season and geographic region. For instance, allergyagainst the birch pollen (Betula verrucosa) is present in the Northern Europe, whereasthe allergic reaction to the olive tree pollen (Olea europea) is widespread in theSouthern Europe and Mediterranean. However, besides the well-known sources ofallergens, it is not clear if pollens from other plant species that are well distributedacross the continent are capable of eliciting the allergic response. In this doctoral thesis,the allergic response to pollen was studied by using two model systems. In one, theallergic response of human patients against the linden pollen was characterized, while inthe other, the canine allergy in Serbia against the pollen from short ragweed was studied.Herein, a detailed characterization of the allergenic potential of the linden pollenamong human patients is presented for the first time. Also, results supporting the crossreactivitybetween the pollens from the linden, birch, and olive trees in patients fromdistinct climate and geographic regions in Europe are shown. The findings suggest thatthe linden tree is a potential source of allergens, that there is a significant level of crossreactivitybetween the linden tree pollen and the pollens from other tree species, andthat it is advisable to incorporate the linden pollen into the clinical pallete of allergens...",
publisher = "Универзитет у Београду, Хемијски факултет",
journal = "Универзитет у Београду",
title = "Imunološka karakterizacija polena lipa i modulacija imunskog odgovora prirodnim aromatičnim jedinjenjima, Immunological characterization of the linden pollens and modulation of the immune response by naturally occurring aromatic compounds",
url = "https://hdl.handle.net/21.15107/rcub_nardus_3496"
}

Ognjenović, J.. (2013). Imunološka karakterizacija polena lipa i modulacija imunskog odgovora prirodnim aromatičnim jedinjenjima. in Универзитет у Београду
Универзитет у Београду, Хемијски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_3496

Ognjenović J. Imunološka karakterizacija polena lipa i modulacija imunskog odgovora prirodnim aromatičnim jedinjenjima. in Универзитет у Београду. 2013;.
https://hdl.handle.net/21.15107/rcub_nardus_3496 .

Ognjenović, Jana, "Imunološka karakterizacija polena lipa i modulacija imunskog odgovora prirodnim aromatičnim jedinjenjima" in Универзитет у Београду (2013),
https://hdl.handle.net/21.15107/rcub_nardus_3496 .

TY  - DATA
AU  - Stojadinović, Marija M.
AU  - Radosavljević, Jelena
AU  - Ognjenović, Jana
AU  - Mihailović-Vesić, Jelena
AU  - Prodić, Ivana
AU  - Stanić-Vučinić, Dragana
AU  - Ćirković-Veličković, Tanja
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2905
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Supplementary data for the article: Stojadinovic, M.; Radosavljevic, J.; Ognjenovic, J.; Vesic, J.; Prodic, I.; Stanic-Vucinic, D.; Cirkovic Velickovic, T. Binding Affinity between Dietary Polyphenols and β-Lactoglobulin Negatively Correlates with the Protein Susceptibility to Digestion and Total Antioxidant Activity of Complexes Formed. Food Chemistry 2013, 136 (3–4), 1263–1271. https://doi.org/10.1016/j.foodchem.2012.09.040
UR  - https://hdl.handle.net/21.15107/rcub_cherry_2905
ER  - 

@misc{
author = "Stojadinović, Marija M. and Radosavljević, Jelena and Ognjenović, Jana and Mihailović-Vesić, Jelena and Prodić, Ivana and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja",
year = "2013",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Supplementary data for the article: Stojadinovic, M.; Radosavljevic, J.; Ognjenovic, J.; Vesic, J.; Prodic, I.; Stanic-Vucinic, D.; Cirkovic Velickovic, T. Binding Affinity between Dietary Polyphenols and β-Lactoglobulin Negatively Correlates with the Protein Susceptibility to Digestion and Total Antioxidant Activity of Complexes Formed. Food Chemistry 2013, 136 (3–4), 1263–1271. https://doi.org/10.1016/j.foodchem.2012.09.040",
url = "https://hdl.handle.net/21.15107/rcub_cherry_2905"
}

Stojadinović, M. M., Radosavljević, J., Ognjenović, J., Mihailović-Vesić, J., Prodić, I., Stanić-Vučinić, D.,& Ćirković-Veličković, T.. (2013). Supplementary data for the article: Stojadinovic, M.; Radosavljevic, J.; Ognjenovic, J.; Vesic, J.; Prodic, I.; Stanic-Vucinic, D.; Cirkovic Velickovic, T. Binding Affinity between Dietary Polyphenols and β-Lactoglobulin Negatively Correlates with the Protein Susceptibility to Digestion and Total Antioxidant Activity of Complexes Formed. Food Chemistry 2013, 136 (3–4), 1263–1271. https://doi.org/10.1016/j.foodchem.2012.09.040. in Food Chemistry
Elsevier Sci Ltd, Oxford..
https://hdl.handle.net/21.15107/rcub_cherry_2905

Stojadinović MM, Radosavljević J, Ognjenović J, Mihailović-Vesić J, Prodić I, Stanić-Vučinić D, Ćirković-Veličković T. Supplementary data for the article: Stojadinovic, M.; Radosavljevic, J.; Ognjenovic, J.; Vesic, J.; Prodic, I.; Stanic-Vucinic, D.; Cirkovic Velickovic, T. Binding Affinity between Dietary Polyphenols and β-Lactoglobulin Negatively Correlates with the Protein Susceptibility to Digestion and Total Antioxidant Activity of Complexes Formed. Food Chemistry 2013, 136 (3–4), 1263–1271. https://doi.org/10.1016/j.foodchem.2012.09.040. in Food Chemistry. 2013;.
https://hdl.handle.net/21.15107/rcub_cherry_2905 .

Stojadinović, Marija M., Radosavljević, Jelena, Ognjenović, Jana, Mihailović-Vesić, Jelena, Prodić, Ivana, Stanić-Vučinić, Dragana, Ćirković-Veličković, Tanja, "Supplementary data for the article: Stojadinovic, M.; Radosavljevic, J.; Ognjenovic, J.; Vesic, J.; Prodic, I.; Stanic-Vucinic, D.; Cirkovic Velickovic, T. Binding Affinity between Dietary Polyphenols and β-Lactoglobulin Negatively Correlates with the Protein Susceptibility to Digestion and Total Antioxidant Activity of Complexes Formed. Food Chemistry 2013, 136 (3–4), 1263–1271. https://doi.org/10.1016/j.foodchem.2012.09.040" in Food Chemistry (2013),
https://hdl.handle.net/21.15107/rcub_cherry_2905 .

TY  - JOUR
AU  - Stojadinović, Marija M.
AU  - Radosavljević, Jelena
AU  - Ognjenović, Jana
AU  - Mihailović-Vesić, Jelena
AU  - Prodić, Ivana
AU  - Stanić-Vučinić, Dragana
AU  - Ćirković-Veličković, Tanja
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1577
AB  - Non-covalent interactions between beta-lactoglobulin (BLG) and polyphenol extracts of teas, coffee and cocoa were studied by fluorescence and CD spectroscopy at pH values of the gastrointestinal tract (GIT). The biological implications of non-covalent binding of polyphenols to BLG were investigated by in vitro pepsin and pancreatin digestibility assay and ABTS radical scavenging activity of complexes formed. The polyphenol-BLG systems were stable at pH values of the GIT. The most profound effect of pH on binding affinity was observed for polyphenol extracts rich in phenolic acids. Stronger non-covalent interactions delayed pepsin and pancreatin digestion of BLG and induced beta-sheet to alpha-helix transition at neutral pH. All polyphenols tested protected protein secondary structure at an extremely acidic pH of 1.2. A positive correlation was found between the strength of protein-polyphenol interactions and (a) half time of protein decay in gastric conditions (R-2 = 0.85), (b) masking of total antioxidant capacity of protein-polyphenol complexes (R-2 = 0.95).
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Binding affinity between dietary polyphenols and beta-lactoglobulin negatively correlates with the protein susceptibility to digestion and total antioxidant activity of complexes formed
VL  - 136
IS  - 3-4
SP  - 1263
EP  - 1271
DO  - 10.1016/j.foodchem.2012.09.040
ER  - 

@article{
author = "Stojadinović, Marija M. and Radosavljević, Jelena and Ognjenović, Jana and Mihailović-Vesić, Jelena and Prodić, Ivana and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja",
year = "2013",
abstract = "Non-covalent interactions between beta-lactoglobulin (BLG) and polyphenol extracts of teas, coffee and cocoa were studied by fluorescence and CD spectroscopy at pH values of the gastrointestinal tract (GIT). The biological implications of non-covalent binding of polyphenols to BLG were investigated by in vitro pepsin and pancreatin digestibility assay and ABTS radical scavenging activity of complexes formed. The polyphenol-BLG systems were stable at pH values of the GIT. The most profound effect of pH on binding affinity was observed for polyphenol extracts rich in phenolic acids. Stronger non-covalent interactions delayed pepsin and pancreatin digestion of BLG and induced beta-sheet to alpha-helix transition at neutral pH. All polyphenols tested protected protein secondary structure at an extremely acidic pH of 1.2. A positive correlation was found between the strength of protein-polyphenol interactions and (a) half time of protein decay in gastric conditions (R-2 = 0.85), (b) masking of total antioxidant capacity of protein-polyphenol complexes (R-2 = 0.95).",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Binding affinity between dietary polyphenols and beta-lactoglobulin negatively correlates with the protein susceptibility to digestion and total antioxidant activity of complexes formed",
volume = "136",
number = "3-4",
pages = "1263-1271",
doi = "10.1016/j.foodchem.2012.09.040"
}

Stojadinović, M. M., Radosavljević, J., Ognjenović, J., Mihailović-Vesić, J., Prodić, I., Stanić-Vučinić, D.,& Ćirković-Veličković, T.. (2013). Binding affinity between dietary polyphenols and beta-lactoglobulin negatively correlates with the protein susceptibility to digestion and total antioxidant activity of complexes formed. in Food Chemistry
Elsevier Sci Ltd, Oxford., 136(3-4), 1263-1271.
https://doi.org/10.1016/j.foodchem.2012.09.040

Stojadinović MM, Radosavljević J, Ognjenović J, Mihailović-Vesić J, Prodić I, Stanić-Vučinić D, Ćirković-Veličković T. Binding affinity between dietary polyphenols and beta-lactoglobulin negatively correlates with the protein susceptibility to digestion and total antioxidant activity of complexes formed. in Food Chemistry. 2013;136(3-4):1263-1271.
doi:10.1016/j.foodchem.2012.09.040 .

Stojadinović, Marija M., Radosavljević, Jelena, Ognjenović, Jana, Mihailović-Vesić, Jelena, Prodić, Ivana, Stanić-Vučinić, Dragana, Ćirković-Veličković, Tanja, "Binding affinity between dietary polyphenols and beta-lactoglobulin negatively correlates with the protein susceptibility to digestion and total antioxidant activity of complexes formed" in Food Chemistry, 136, no. 3-4 (2013):1263-1271,
https://doi.org/10.1016/j.foodchem.2012.09.040 . .

TY  - JOUR
AU  - Ognjenović, Jana
AU  - Milčić-Matić, Natalija
AU  - Smiljanić, Katarina
AU  - Vučković, Olga
AU  - Burazer, Lidija M.
AU  - Popović, Nikola
AU  - Stanić-Vučinić, Dragana
AU  - Ćirković-Veličković, Tanja
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1391
AB  - Canine atopic dermatitis (CAD) is an immune system disorder that affects 10-15% of the canine population. Short ragweed (Ambrosia artemisiifolia) pollen represents one of the major seasonal sources of allergenic pollen proteins in Europe, particularly in the Pannonian valley of the Balkan region. In Serbia, about 66% of atopic dogs showed a positive intradermal skin test with its pollen extract, which is second to house dust mites. Therefore, characterization of Ambrosia artemisiifolia pollen components, in terms of defining major and minor allergens that induce clinically manifested allergic reaction in dogs, is important for valid diagnosis and efficient therapy. This study has, for the first time, characterized and identified major Ambrosia artemisiifolia allergens in CAD, using an immunoproteomic approach. To assess the prevalence of specific IgE in electrophoretically separated ragweed pollen proteins, individual reactivity of sera from dogs with CAD was analyzed and compared to the reactivity of sera from healthy dogs in the non-reducing conditions, which were found optimal for specific canine IgE detection. A specific IgE band (38 kDa) was recognized as the most dominant allergen in CAD, occurring in 81% of positive dog's sera. 2-D immunoblotting followed by a mass spectrometry peptide fingerprint analyses with pooled canine and human atopic sera, revealed that 38 kDa major Ambrosia atremisiifolia allergens in CAD were all five isoallergens of the Amb a 1 group (antigen E), including the previously named Amb a 2 (antigen K). In contrast to canine sera, human atopic sera also recognized lower mass allergens such as the 13 fragment of Amb a 1 and profilins (Amb a 8 variants). The most prominent ragweed proteins in CAD, represent, as in humans, variants of all five isoallergens of the Amb a 1 group (pectate lyase): Amb a 1.0101 and its natural variant E1XUL2, Amb a 1.0202, 1.0304, 1.0402 and the natural variant of Amb a 1.0501, E1XUM0, as well as the a fragment of pollen allergen Amb a 1.0201.
PB  - Elsevier Science Bv, Amsterdam
T2  - Veterinary Immunology and Immunopathology
T1  - Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis
VL  - 155
IS  - 1-2
SP  - 38
EP  - 47
DO  - 10.1016/j.vetimm.2013.06.005
ER  - 

@article{
author = "Ognjenović, Jana and Milčić-Matić, Natalija and Smiljanić, Katarina and Vučković, Olga and Burazer, Lidija M. and Popović, Nikola and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja",
year = "2013",
abstract = "Canine atopic dermatitis (CAD) is an immune system disorder that affects 10-15% of the canine population. Short ragweed (Ambrosia artemisiifolia) pollen represents one of the major seasonal sources of allergenic pollen proteins in Europe, particularly in the Pannonian valley of the Balkan region. In Serbia, about 66% of atopic dogs showed a positive intradermal skin test with its pollen extract, which is second to house dust mites. Therefore, characterization of Ambrosia artemisiifolia pollen components, in terms of defining major and minor allergens that induce clinically manifested allergic reaction in dogs, is important for valid diagnosis and efficient therapy. This study has, for the first time, characterized and identified major Ambrosia artemisiifolia allergens in CAD, using an immunoproteomic approach. To assess the prevalence of specific IgE in electrophoretically separated ragweed pollen proteins, individual reactivity of sera from dogs with CAD was analyzed and compared to the reactivity of sera from healthy dogs in the non-reducing conditions, which were found optimal for specific canine IgE detection. A specific IgE band (38 kDa) was recognized as the most dominant allergen in CAD, occurring in 81% of positive dog's sera. 2-D immunoblotting followed by a mass spectrometry peptide fingerprint analyses with pooled canine and human atopic sera, revealed that 38 kDa major Ambrosia atremisiifolia allergens in CAD were all five isoallergens of the Amb a 1 group (antigen E), including the previously named Amb a 2 (antigen K). In contrast to canine sera, human atopic sera also recognized lower mass allergens such as the 13 fragment of Amb a 1 and profilins (Amb a 8 variants). The most prominent ragweed proteins in CAD, represent, as in humans, variants of all five isoallergens of the Amb a 1 group (pectate lyase): Amb a 1.0101 and its natural variant E1XUL2, Amb a 1.0202, 1.0304, 1.0402 and the natural variant of Amb a 1.0501, E1XUM0, as well as the a fragment of pollen allergen Amb a 1.0201.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Veterinary Immunology and Immunopathology",
title = "Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis",
volume = "155",
number = "1-2",
pages = "38-47",
doi = "10.1016/j.vetimm.2013.06.005"
}

Ognjenović, J., Milčić-Matić, N., Smiljanić, K., Vučković, O., Burazer, L. M., Popović, N., Stanić-Vučinić, D.,& Ćirković-Veličković, T.. (2013). Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis. in Veterinary Immunology and Immunopathology
Elsevier Science Bv, Amsterdam., 155(1-2), 38-47.
https://doi.org/10.1016/j.vetimm.2013.06.005

Ognjenović J, Milčić-Matić N, Smiljanić K, Vučković O, Burazer LM, Popović N, Stanić-Vučinić D, Ćirković-Veličković T. Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis. in Veterinary Immunology and Immunopathology. 2013;155(1-2):38-47.
doi:10.1016/j.vetimm.2013.06.005 .

Ognjenović, Jana, Milčić-Matić, Natalija, Smiljanić, Katarina, Vučković, Olga, Burazer, Lidija M., Popović, Nikola, Stanić-Vučinić, Dragana, Ćirković-Veličković, Tanja, "Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis" in Veterinary Immunology and Immunopathology, 155, no. 1-2 (2013):38-47,
https://doi.org/10.1016/j.vetimm.2013.06.005 . .

TY  - JOUR
AU  - Milčić-Matić, Natalija
AU  - Ognjenović, Jana
AU  - Burazer, Lidija M.
AU  - Blagojević, G.
AU  - Popović, Nikola
AU  - Lazarevic, M.
AU  - Stanić-Vučinić, Dragana
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1407
AB  - Common ragweed (Ambrosia atremisiifolia) is one of the most frequent causes of pollen-induced allergic reactions both in humans and dogs. It has not been defined yet, what is the major allergen(s) to which most dogs allergic to ragweed show a positive result on intradermal skin test (IDST). In the present study sensitization to Ambrosia artemisiifolia pollen allergens in dogs with atopic dermatitis was examined with both in vivo and in vitro tests, including IDST and serum allergen specific IgE test. Detection of specific-IgE antibodies against ragweed allergens by immunoblotting in the sera of allergic dogs was optimized, as well. Dogs that were positive, as judged by IDST reactions to ragweed pollen allergens, also had alergen specific IgE antibodies in their sera. Results indicate that major allergens of A. artemisifolia pollen in dogs are Amb a 1 and Amb a 2. Further characterization of ragweed allergens is needed before they could potentially be used in intradermal testing or allergen immunotherapy in affected dogs. Also, we evaluated new Favrots diagnostic criteria for canine atopic dermatitis in dogs allergic to Ambrosia atremisiifolia pollen. It might be concluded that proposed criteria are of great assistance for seting up suspected diagnosis of canine atopic dermatitis, after ruling out other pruritic dermatoses.
AB  - Kratka ambrozija (Ambrosia artemisiifolia) je jedan od najčešćih uzročnika alergijskih reakcija izazvanih polenom kod ljudi i pasa. Još uvek nije definisano koji je glavni alergen (i), na koji, većina pasa alergičnih na polen ambrozije, ispoljava pozitivnu reakciju na intradermalnom testu. U ovoj studiji je ispitana senzibilizacija na polen ove biljke kod pasa sa simptomima atopijskog dermatitisa in vivo i in vitro testovima, uključujući intradermalni test i dokazivanje prisustva alergen specifičnih IgE antitela u serumu. Optimizovani su uslovi za detekciju IgE specifičnih antitela iz seruma pasa alergičnih na polen ambrozije imunoblot tehnikom. Psi koji su imali pozitivnu reakciju na polen ove biljke na intradermalnom testu, takođe su imali specifična IgE antitela u serumu. Dobijeni rezultati ukazuju da su glavni alergeni Ambrosia artemisiifolia kod pasa Amb a 1 i Amb a 2. Neophodna je dalja karakterizacija alergena ambrozije kako bi se oni mogli primeniti pri rutinskom intradermalnom testiranju ili u alergen specifičnoj imunoterapiji obolelih pasa. Takođe je razmatrana i validnost Favrotovih dijagnostičkih kriterijuma kod pasa alergičnih na polen ambrozije. Može se zaključiti da su predloženi kriterijumi od velike pomoći u postavljanju suspektne dijagnoze atopijskog dermatitisa pasa, nakon isključenja drugih pruritičnih dermatoza.
PB  - De Gruyter Poland Sp Zoo, Warsaw
T2  - Acta Veterinaria, Beograd
T1  - Evaluation of Criteria for Diagnosis of Atopic Dermatitis and Detection of Allergen Specific Ige Antibodies in Dogs Allergic to Ambrosia Artemisiifolia Pollen
T1  - Evaluacija kriterijuma za dijagnozu atopijskog dermatitisa i detekcija alergen specifičnih IgE antitela kod pasa alergičnih na polen biljke Ambrosia artemisiifolia
VL  - 63
IS  - 4
SP  - 437
EP  - 451
DO  - 10.2298/AVB1304437M
ER  - 

@article{
author = "Milčić-Matić, Natalija and Ognjenović, Jana and Burazer, Lidija M. and Blagojević, G. and Popović, Nikola and Lazarevic, M. and Stanić-Vučinić, Dragana",
year = "2013",
abstract = "Common ragweed (Ambrosia atremisiifolia) is one of the most frequent causes of pollen-induced allergic reactions both in humans and dogs. It has not been defined yet, what is the major allergen(s) to which most dogs allergic to ragweed show a positive result on intradermal skin test (IDST). In the present study sensitization to Ambrosia artemisiifolia pollen allergens in dogs with atopic dermatitis was examined with both in vivo and in vitro tests, including IDST and serum allergen specific IgE test. Detection of specific-IgE antibodies against ragweed allergens by immunoblotting in the sera of allergic dogs was optimized, as well. Dogs that were positive, as judged by IDST reactions to ragweed pollen allergens, also had alergen specific IgE antibodies in their sera. Results indicate that major allergens of A. artemisifolia pollen in dogs are Amb a 1 and Amb a 2. Further characterization of ragweed allergens is needed before they could potentially be used in intradermal testing or allergen immunotherapy in affected dogs. Also, we evaluated new Favrots diagnostic criteria for canine atopic dermatitis in dogs allergic to Ambrosia atremisiifolia pollen. It might be concluded that proposed criteria are of great assistance for seting up suspected diagnosis of canine atopic dermatitis, after ruling out other pruritic dermatoses., Kratka ambrozija (Ambrosia artemisiifolia) je jedan od najčešćih uzročnika alergijskih reakcija izazvanih polenom kod ljudi i pasa. Još uvek nije definisano koji je glavni alergen (i), na koji, većina pasa alergičnih na polen ambrozije, ispoljava pozitivnu reakciju na intradermalnom testu. U ovoj studiji je ispitana senzibilizacija na polen ove biljke kod pasa sa simptomima atopijskog dermatitisa in vivo i in vitro testovima, uključujući intradermalni test i dokazivanje prisustva alergen specifičnih IgE antitela u serumu. Optimizovani su uslovi za detekciju IgE specifičnih antitela iz seruma pasa alergičnih na polen ambrozije imunoblot tehnikom. Psi koji su imali pozitivnu reakciju na polen ove biljke na intradermalnom testu, takođe su imali specifična IgE antitela u serumu. Dobijeni rezultati ukazuju da su glavni alergeni Ambrosia artemisiifolia kod pasa Amb a 1 i Amb a 2. Neophodna je dalja karakterizacija alergena ambrozije kako bi se oni mogli primeniti pri rutinskom intradermalnom testiranju ili u alergen specifičnoj imunoterapiji obolelih pasa. Takođe je razmatrana i validnost Favrotovih dijagnostičkih kriterijuma kod pasa alergičnih na polen ambrozije. Može se zaključiti da su predloženi kriterijumi od velike pomoći u postavljanju suspektne dijagnoze atopijskog dermatitisa pasa, nakon isključenja drugih pruritičnih dermatoza.",
publisher = "De Gruyter Poland Sp Zoo, Warsaw",
journal = "Acta Veterinaria, Beograd",
title = "Evaluation of Criteria for Diagnosis of Atopic Dermatitis and Detection of Allergen Specific Ige Antibodies in Dogs Allergic to Ambrosia Artemisiifolia Pollen, Evaluacija kriterijuma za dijagnozu atopijskog dermatitisa i detekcija alergen specifičnih IgE antitela kod pasa alergičnih na polen biljke Ambrosia artemisiifolia",
volume = "63",
number = "4",
pages = "437-451",
doi = "10.2298/AVB1304437M"
}

Milčić-Matić, N., Ognjenović, J., Burazer, L. M., Blagojević, G., Popović, N., Lazarevic, M.,& Stanić-Vučinić, D.. (2013). Evaluation of Criteria for Diagnosis of Atopic Dermatitis and Detection of Allergen Specific Ige Antibodies in Dogs Allergic to Ambrosia Artemisiifolia Pollen. in Acta Veterinaria, Beograd
De Gruyter Poland Sp Zoo, Warsaw., 63(4), 437-451.
https://doi.org/10.2298/AVB1304437M

Milčić-Matić N, Ognjenović J, Burazer LM, Blagojević G, Popović N, Lazarevic M, Stanić-Vučinić D. Evaluation of Criteria for Diagnosis of Atopic Dermatitis and Detection of Allergen Specific Ige Antibodies in Dogs Allergic to Ambrosia Artemisiifolia Pollen. in Acta Veterinaria, Beograd. 2013;63(4):437-451.
doi:10.2298/AVB1304437M .

Milčić-Matić, Natalija, Ognjenović, Jana, Burazer, Lidija M., Blagojević, G., Popović, Nikola, Lazarevic, M., Stanić-Vučinić, Dragana, "Evaluation of Criteria for Diagnosis of Atopic Dermatitis and Detection of Allergen Specific Ige Antibodies in Dogs Allergic to Ambrosia Artemisiifolia Pollen" in Acta Veterinaria, Beograd, 63, no. 4 (2013):437-451,
https://doi.org/10.2298/AVB1304437M . .

TY  - JOUR
AU  - Stanić-Vučinić, Dragana
AU  - Stojadinović, Marija M.
AU  - Atanasković-Marković, Marina
AU  - Ognjenović, Jana
AU  - Gronlund, Hans
AU  - van Hage, Marianne
AU  - Lantto, Raija
AU  - Sancho, Ana I.
AU  - Ćirković-Veličković, Tanja
PY  - 2012
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1561
AB  - Scope The aim of this work was to investigate the effects of high-intensity ultrasound (sonication), on the structure and allergenicity of the major cow's milk allergen, beta-lactoglobulin (BLG). Methods and results Structural changes upon sonication of BLG were monitored by circular dichroism spectroscopy, tryptophan emission fluorescence, hydrophobic dye and retinol binding, as well as digestibility and phenol-oxidase cross-linking capacity. Allergenicity was monitored in individual patients sera, basophil activation test, and skin prick testing in 41 cow's milk allergy patients. Uncontrolled local temperature changes induced modifications in BLG secondary structure accompanied by formation of dimers, trimers, and oligomers of BLG that were more digestible by pepsin and had reduced retinol binding. Controlled temperature conditions induced changes in secondary structure of BLG without causing formation of oligomers, or changing protein's capacity to bind retinol. Both sonicated forms of BLG had more exposed hydrophobic surfaces than native BLG and underwent facilitated cross-linking reaction with phenol-oxidase. Sonication had a minor effect on IgE-binding properties of BLG. Conclusion Sonication-induced structural changes in major whey allergen were not clinically significant in cow's milk allergy patients. Ultrasound can be a safe procedure for dairy processing as it maintains the nutritional value and does not increase allergenic potential of BLG.
PB  - Wiley-Blackwell, Hoboken
T2  - Molecular Nutrition and Food Research
T1  - Structural changes and allergenic properties of beta-lactoglobulin upon exposure to high-intensity ultrasound
VL  - 56
IS  - 12
SP  - 1894
EP  - 1905
DO  - 10.1002/mnfr.201200179
ER  - 

@article{
author = "Stanić-Vučinić, Dragana and Stojadinović, Marija M. and Atanasković-Marković, Marina and Ognjenović, Jana and Gronlund, Hans and van Hage, Marianne and Lantto, Raija and Sancho, Ana I. and Ćirković-Veličković, Tanja",
year = "2012",
abstract = "Scope The aim of this work was to investigate the effects of high-intensity ultrasound (sonication), on the structure and allergenicity of the major cow's milk allergen, beta-lactoglobulin (BLG). Methods and results Structural changes upon sonication of BLG were monitored by circular dichroism spectroscopy, tryptophan emission fluorescence, hydrophobic dye and retinol binding, as well as digestibility and phenol-oxidase cross-linking capacity. Allergenicity was monitored in individual patients sera, basophil activation test, and skin prick testing in 41 cow's milk allergy patients. Uncontrolled local temperature changes induced modifications in BLG secondary structure accompanied by formation of dimers, trimers, and oligomers of BLG that were more digestible by pepsin and had reduced retinol binding. Controlled temperature conditions induced changes in secondary structure of BLG without causing formation of oligomers, or changing protein's capacity to bind retinol. Both sonicated forms of BLG had more exposed hydrophobic surfaces than native BLG and underwent facilitated cross-linking reaction with phenol-oxidase. Sonication had a minor effect on IgE-binding properties of BLG. Conclusion Sonication-induced structural changes in major whey allergen were not clinically significant in cow's milk allergy patients. Ultrasound can be a safe procedure for dairy processing as it maintains the nutritional value and does not increase allergenic potential of BLG.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Molecular Nutrition and Food Research",
title = "Structural changes and allergenic properties of beta-lactoglobulin upon exposure to high-intensity ultrasound",
volume = "56",
number = "12",
pages = "1894-1905",
doi = "10.1002/mnfr.201200179"
}

Stanić-Vučinić, D., Stojadinović, M. M., Atanasković-Marković, M., Ognjenović, J., Gronlund, H., van Hage, M., Lantto, R., Sancho, A. I.,& Ćirković-Veličković, T.. (2012). Structural changes and allergenic properties of beta-lactoglobulin upon exposure to high-intensity ultrasound. in Molecular Nutrition and Food Research
Wiley-Blackwell, Hoboken., 56(12), 1894-1905.
https://doi.org/10.1002/mnfr.201200179

Stanić-Vučinić D, Stojadinović MM, Atanasković-Marković M, Ognjenović J, Gronlund H, van Hage M, Lantto R, Sancho AI, Ćirković-Veličković T. Structural changes and allergenic properties of beta-lactoglobulin upon exposure to high-intensity ultrasound. in Molecular Nutrition and Food Research. 2012;56(12):1894-1905.
doi:10.1002/mnfr.201200179 .

Stanić-Vučinić, Dragana, Stojadinović, Marija M., Atanasković-Marković, Marina, Ognjenović, Jana, Gronlund, Hans, van Hage, Marianne, Lantto, Raija, Sancho, Ana I., Ćirković-Veličković, Tanja, "Structural changes and allergenic properties of beta-lactoglobulin upon exposure to high-intensity ultrasound" in Molecular Nutrition and Food Research, 56, no. 12 (2012):1894-1905,
https://doi.org/10.1002/mnfr.201200179 . .

TY  - JOUR
AU  - Ognjenović, Jana
AU  - Tantoush, Ziyad Omar
AU  - Jankov, Ratko M.
AU  - Ćirković-Veličković, Tanja
AU  - Vukmirica, Jelena
PY  - 2012
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1531
AB  - The conditions required for the isolation of high quality total RNA from European linden (Tilia cordata) leaves and pollen were determined. Pure total RNA was isolated from linden leaves utilizing a Qiagen plant mini kit, while the total RNA isolated from linden pollen using this method was degraded. Successful isolation of total RNA from both linden pollen and leaves, however, was achieved following TRIzol (TM) preparation of the total RNA. The total RNA isolated using TRIzol (TM) was contaminated with genomic DNA but treatment with the enzyme DNase, in solution or on-column, efficiently removed the genomic DNA. Furthermore, the conditions for the elimination of genomic DNA contamination on-column and isolation of pure total RNA from leaves were optimized. The isolated total RNA from both leaves and pollen was used successfully in first-and second-strand cDNA synthesis reactions and in a reverse transcription polymerase chain reaction (RT-PCR), demonstrating that the total RNA isolated using this method was functional. In conclusion, pure and functional total RNA from T. cordata leaves and pollen (27.8 +/- 7.9 mu g g(-1) leaves; 25.7 +/- 1.1 mu g g(-1) pollen) could be obtained and was suitable for application in further molecular biology studies.
AB  - Uspostavljeni su uslovi za izolovanje ukupne RNK iz lišća i polena evropske lipe (Tilia cordata). Korišćenjem komercijalno dostupnog pribora za izolovanje RNK iz biljaka izolovana je čista ukupna RNK iz lišća lipe, dok je korišćenjem iste metode dobijena degradirana RNK iz polena lipe. Uspešno izolovanje RNK iz lišća i polena je dobijeno korišćenjem TRIzol reagensa. RNK izolovana ovim metodom je kontaminirana genomskom DNK, koja je uspešno eliminisana korišćenjem enzima DNaze. Dalje su optimizovani i uslovi uklanjanja genomske DNK pomoću DNaze. Izolovana ukupna RNK iz oba izvora je dalje uspešno iskorišćena za sintezu prvog i drugog lanca klonske DNK, kao i u reverzno-transkriptivnoj PCR reakciji, dokazujući time da je korišćenjem ovog metoda izolovana funkcionalna ukupna RNK. U zaključku, dobijena je čista i funkcionalna RNK iz lišća i polena T. cordata (27,8±7,9 μg g-1 lišća; 25,7±1,1 μg g-1 polena) koja se može koristiti u daljim molekularno-biološkim istraživanjima.
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Isolation of functional total RNA from Tilia cordata leaves and pollen
T1  - Izolovanje funkcionalne ukupne RNK iz lišća i polena lipe (Tilia cordata)
VL  - 77
IS  - 8
SP  - 1003
EP  - 1012
DO  - 10.2298/JSC111130018O
ER  - 

@article{
author = "Ognjenović, Jana and Tantoush, Ziyad Omar and Jankov, Ratko M. and Ćirković-Veličković, Tanja and Vukmirica, Jelena",
year = "2012",
abstract = "The conditions required for the isolation of high quality total RNA from European linden (Tilia cordata) leaves and pollen were determined. Pure total RNA was isolated from linden leaves utilizing a Qiagen plant mini kit, while the total RNA isolated from linden pollen using this method was degraded. Successful isolation of total RNA from both linden pollen and leaves, however, was achieved following TRIzol (TM) preparation of the total RNA. The total RNA isolated using TRIzol (TM) was contaminated with genomic DNA but treatment with the enzyme DNase, in solution or on-column, efficiently removed the genomic DNA. Furthermore, the conditions for the elimination of genomic DNA contamination on-column and isolation of pure total RNA from leaves were optimized. The isolated total RNA from both leaves and pollen was used successfully in first-and second-strand cDNA synthesis reactions and in a reverse transcription polymerase chain reaction (RT-PCR), demonstrating that the total RNA isolated using this method was functional. In conclusion, pure and functional total RNA from T. cordata leaves and pollen (27.8 +/- 7.9 mu g g(-1) leaves; 25.7 +/- 1.1 mu g g(-1) pollen) could be obtained and was suitable for application in further molecular biology studies., Uspostavljeni su uslovi za izolovanje ukupne RNK iz lišća i polena evropske lipe (Tilia cordata). Korišćenjem komercijalno dostupnog pribora za izolovanje RNK iz biljaka izolovana je čista ukupna RNK iz lišća lipe, dok je korišćenjem iste metode dobijena degradirana RNK iz polena lipe. Uspešno izolovanje RNK iz lišća i polena je dobijeno korišćenjem TRIzol reagensa. RNK izolovana ovim metodom je kontaminirana genomskom DNK, koja je uspešno eliminisana korišćenjem enzima DNaze. Dalje su optimizovani i uslovi uklanjanja genomske DNK pomoću DNaze. Izolovana ukupna RNK iz oba izvora je dalje uspešno iskorišćena za sintezu prvog i drugog lanca klonske DNK, kao i u reverzno-transkriptivnoj PCR reakciji, dokazujući time da je korišćenjem ovog metoda izolovana funkcionalna ukupna RNK. U zaključku, dobijena je čista i funkcionalna RNK iz lišća i polena T. cordata (27,8±7,9 μg g-1 lišća; 25,7±1,1 μg g-1 polena) koja se može koristiti u daljim molekularno-biološkim istraživanjima.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Isolation of functional total RNA from Tilia cordata leaves and pollen, Izolovanje funkcionalne ukupne RNK iz lišća i polena lipe (Tilia cordata)",
volume = "77",
number = "8",
pages = "1003-1012",
doi = "10.2298/JSC111130018O"
}

Ognjenović, J., Tantoush, Z. O., Jankov, R. M., Ćirković-Veličković, T.,& Vukmirica, J.. (2012). Isolation of functional total RNA from Tilia cordata leaves and pollen. in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 77(8), 1003-1012.
https://doi.org/10.2298/JSC111130018O

Ognjenović J, Tantoush ZO, Jankov RM, Ćirković-Veličković T, Vukmirica J. Isolation of functional total RNA from Tilia cordata leaves and pollen. in Journal of the Serbian Chemical Society. 2012;77(8):1003-1012.
doi:10.2298/JSC111130018O .

Ognjenović, Jana, Tantoush, Ziyad Omar, Jankov, Ratko M., Ćirković-Veličković, Tanja, Vukmirica, Jelena, "Isolation of functional total RNA from Tilia cordata leaves and pollen" in Journal of the Serbian Chemical Society, 77, no. 8 (2012):1003-1012,
https://doi.org/10.2298/JSC111130018O . .

TY  - JOUR
AU  - Tantoush, Ziyad
AU  - Stanić, Dragana
AU  - Stojadinović, Marija M.
AU  - Ognjenović, Jana
AU  - Mihajlovic, Luka
AU  - Atanasković-Marković, Marina
AU  - Ćirković-Veličković, Tanja
PY  - 2011
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1137
AB  - beta-Lactoglobulin (BLG) is an important nutrient of dairy products, but it represents a serious health risk in patients allergic to milk. Sour cherry (Prunus cerasus L) extract (SCE) is frequently added as a natural food colour in composite foods, such as fruit yogurt, ice creams, frappes and milkshakes. The aim of this study was to investigate the potential of laccase to cross-link BLG in the presence of an SCE and to characterise the obtained products for their bioactivity. Laccase cross-linked BLG in the presence of sour cherry phenolics. In a basophil-activation assay, the allergenicity of the cross-linked protein was shown to decrease in all nine cow's milk-allergic patients, while digestibility of the remaining monomeric BLG in simulated conditions of the gastrointestinal tract increased. Tryptic peptides became immediately available in BLG treated by laccase and laccase/SCE. The hydrolysates obtained by trypsin digestion of BLG/laccase/SCE showed an increase of 57% in radical-scavenging activity, compared to the control BLG. Enzymatic processing and usage of natural phenolic extracts as mediators of enzymatic reaction may improve BLG safety and the availability of peptides following digestion, while conserving its bioactivity.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Digestibility and allergenicity of beta-lactoglobulin following laccase-mediated cross-linking in the presence of sour cherry phenolics
VL  - 125
IS  - 1
SP  - 84
EP  - 91
DO  - 10.1016/j.foodchem.2010.08.040
ER  - 

@article{
author = "Tantoush, Ziyad and Stanić, Dragana and Stojadinović, Marija M. and Ognjenović, Jana and Mihajlovic, Luka and Atanasković-Marković, Marina and Ćirković-Veličković, Tanja",
year = "2011",
abstract = "beta-Lactoglobulin (BLG) is an important nutrient of dairy products, but it represents a serious health risk in patients allergic to milk. Sour cherry (Prunus cerasus L) extract (SCE) is frequently added as a natural food colour in composite foods, such as fruit yogurt, ice creams, frappes and milkshakes. The aim of this study was to investigate the potential of laccase to cross-link BLG in the presence of an SCE and to characterise the obtained products for their bioactivity. Laccase cross-linked BLG in the presence of sour cherry phenolics. In a basophil-activation assay, the allergenicity of the cross-linked protein was shown to decrease in all nine cow's milk-allergic patients, while digestibility of the remaining monomeric BLG in simulated conditions of the gastrointestinal tract increased. Tryptic peptides became immediately available in BLG treated by laccase and laccase/SCE. The hydrolysates obtained by trypsin digestion of BLG/laccase/SCE showed an increase of 57% in radical-scavenging activity, compared to the control BLG. Enzymatic processing and usage of natural phenolic extracts as mediators of enzymatic reaction may improve BLG safety and the availability of peptides following digestion, while conserving its bioactivity.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Digestibility and allergenicity of beta-lactoglobulin following laccase-mediated cross-linking in the presence of sour cherry phenolics",
volume = "125",
number = "1",
pages = "84-91",
doi = "10.1016/j.foodchem.2010.08.040"
}

Tantoush, Z., Stanić, D., Stojadinović, M. M., Ognjenović, J., Mihajlovic, L., Atanasković-Marković, M.,& Ćirković-Veličković, T.. (2011). Digestibility and allergenicity of beta-lactoglobulin following laccase-mediated cross-linking in the presence of sour cherry phenolics. in Food Chemistry
Elsevier Sci Ltd, Oxford., 125(1), 84-91.
https://doi.org/10.1016/j.foodchem.2010.08.040

Tantoush Z, Stanić D, Stojadinović MM, Ognjenović J, Mihajlovic L, Atanasković-Marković M, Ćirković-Veličković T. Digestibility and allergenicity of beta-lactoglobulin following laccase-mediated cross-linking in the presence of sour cherry phenolics. in Food Chemistry. 2011;125(1):84-91.
doi:10.1016/j.foodchem.2010.08.040 .

Tantoush, Ziyad, Stanić, Dragana, Stojadinović, Marija M., Ognjenović, Jana, Mihajlovic, Luka, Atanasković-Marković, Marina, Ćirković-Veličković, Tanja, "Digestibility and allergenicity of beta-lactoglobulin following laccase-mediated cross-linking in the presence of sour cherry phenolics" in Food Chemistry, 125, no. 1 (2011):84-91,
https://doi.org/10.1016/j.foodchem.2010.08.040 . .

TY  - JOUR
AU  - Tantoush, Ziyad
AU  - Mihajlovic, Luka
AU  - Kravić, Bojana
AU  - Ognjenović, Jana
AU  - Jankov, Ratko M.
AU  - Ćirković-Veličković, Tanja
AU  - Stanić-Vučinić, Dragana
PY  - 2011
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1174
AB  - beta-Lactoglobulin (BLG) is an important nutrient of dairy products and an important allergen in cow's milk allergy. The aim of this study was to investigate the potential of laccase to cross-link BLG in the presence of an apple phenolic extract (APE) and to characterize the obtained products for their digestibility by pepsin and pancreatin. The composition of the apple phenolics used for cross-linking was determined by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). The apple phenolic extract contained significant amounts of quercetin glycosides, catechins and chlorogenic acid. The laccase cross-linked BLG in the presence of apple phenolics. The polymerization rendered the protein insoluble in the reaction mixture. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the cross-linking reaction mixture revealed a heterogeneous mixture of high molecular masses (cross-linked BLG), with a fraction of the BLG remaining monomeric. Enzymatic processing of BLG by laccase and apple polyphenols as mediators can decrease the biphasal pepsin pancreatin digestibility of the monomeric and cross-linked protein, thus decreasing its nutritional value. In addition, reduced BLG digestibility can decrease its allergenic potential. Apple polyphenols can find usage in the creation of new, more functional food products, designed to prevent obesity and hypersensitivity-related disorders.
AB  - β-Laktoglobulin (BLG) je važan nutrijent mlečnih proizvoda i važan alergen kod alergija na kravlje mleko. Cilj ove studije je bilo ispitivanje potencijala lakaze da unakrsno poveže BLG u prisustvu fenolnog ekstrakta jabuke (APE), kao i karakterizacija dobijenih proizvoda sa aspekta njihove digestibilnosti pepsinom i pankreatinom. Kompozicija fenola jabuke korišćenih za unakrsno povezivanje određena je pomoću LC-ESI-MS. Fenolni ekstrakt jabuke sadrži znatne količine glikozida kvercetina, katehine i hlorogensku kiselinu. BLG je unakrsno povezan lakazom u prisustvu fenola jabuke, pri čemu je polimerizacija učinila BLG nerastvornim u reakcionoj smeši. SDS-PAGE analiza pokazala je da reakciona smeša sadrži heterogenu smešu velikih molekulskih masa (unakrsno povezan BLG), kao i deo zaostalog monomernog BLG. Enzimsko procesovanje BLG lakazom, u prisustvu polifenola jabuke kao medijatora, može smanjiti bifaznu pepsin-pankreatinsku digestibilnost kako monomernog, tako i unakrsno povezanog BLG, i na taj način smanjiti njegovu nutritivnu vrednost. Takođe, smanjena digestibilnost BLG može smanjiti njegov alergeni potencijal. Polifenoli jabuke mogu se koristiti za kreiranje novih, funkcionalnijih prehrambenih proizvoda, dizajniranih za prevenciju kako gojaznosti, tako i bolesti vezanih za preosetljivost.
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Digestibility of beta-lactoglobulin following cross-linking by Trametes versicolor laccase and apple polyphenols
T1  - Digestibilnost β-laktoglobulina nakon njegovog unakrsnog povezivanja dejstvom lakaze iz Trametes versicolor i polifenola iz jabuke
VL  - 76
IS  - 6
SP  - 847
EP  - 855
DO  - 10.2298/JSC101201077T
ER  - 

@article{
author = "Tantoush, Ziyad and Mihajlovic, Luka and Kravić, Bojana and Ognjenović, Jana and Jankov, Ratko M. and Ćirković-Veličković, Tanja and Stanić-Vučinić, Dragana",
year = "2011",
abstract = "beta-Lactoglobulin (BLG) is an important nutrient of dairy products and an important allergen in cow's milk allergy. The aim of this study was to investigate the potential of laccase to cross-link BLG in the presence of an apple phenolic extract (APE) and to characterize the obtained products for their digestibility by pepsin and pancreatin. The composition of the apple phenolics used for cross-linking was determined by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). The apple phenolic extract contained significant amounts of quercetin glycosides, catechins and chlorogenic acid. The laccase cross-linked BLG in the presence of apple phenolics. The polymerization rendered the protein insoluble in the reaction mixture. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the cross-linking reaction mixture revealed a heterogeneous mixture of high molecular masses (cross-linked BLG), with a fraction of the BLG remaining monomeric. Enzymatic processing of BLG by laccase and apple polyphenols as mediators can decrease the biphasal pepsin pancreatin digestibility of the monomeric and cross-linked protein, thus decreasing its nutritional value. In addition, reduced BLG digestibility can decrease its allergenic potential. Apple polyphenols can find usage in the creation of new, more functional food products, designed to prevent obesity and hypersensitivity-related disorders., β-Laktoglobulin (BLG) je važan nutrijent mlečnih proizvoda i važan alergen kod alergija na kravlje mleko. Cilj ove studije je bilo ispitivanje potencijala lakaze da unakrsno poveže BLG u prisustvu fenolnog ekstrakta jabuke (APE), kao i karakterizacija dobijenih proizvoda sa aspekta njihove digestibilnosti pepsinom i pankreatinom. Kompozicija fenola jabuke korišćenih za unakrsno povezivanje određena je pomoću LC-ESI-MS. Fenolni ekstrakt jabuke sadrži znatne količine glikozida kvercetina, katehine i hlorogensku kiselinu. BLG je unakrsno povezan lakazom u prisustvu fenola jabuke, pri čemu je polimerizacija učinila BLG nerastvornim u reakcionoj smeši. SDS-PAGE analiza pokazala je da reakciona smeša sadrži heterogenu smešu velikih molekulskih masa (unakrsno povezan BLG), kao i deo zaostalog monomernog BLG. Enzimsko procesovanje BLG lakazom, u prisustvu polifenola jabuke kao medijatora, može smanjiti bifaznu pepsin-pankreatinsku digestibilnost kako monomernog, tako i unakrsno povezanog BLG, i na taj način smanjiti njegovu nutritivnu vrednost. Takođe, smanjena digestibilnost BLG može smanjiti njegov alergeni potencijal. Polifenoli jabuke mogu se koristiti za kreiranje novih, funkcionalnijih prehrambenih proizvoda, dizajniranih za prevenciju kako gojaznosti, tako i bolesti vezanih za preosetljivost.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Digestibility of beta-lactoglobulin following cross-linking by Trametes versicolor laccase and apple polyphenols, Digestibilnost β-laktoglobulina nakon njegovog unakrsnog povezivanja dejstvom lakaze iz Trametes versicolor i polifenola iz jabuke",
volume = "76",
number = "6",
pages = "847-855",
doi = "10.2298/JSC101201077T"
}

Tantoush, Z., Mihajlovic, L., Kravić, B., Ognjenović, J., Jankov, R. M., Ćirković-Veličković, T.,& Stanić-Vučinić, D.. (2011). Digestibility of beta-lactoglobulin following cross-linking by Trametes versicolor laccase and apple polyphenols. in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 76(6), 847-855.
https://doi.org/10.2298/JSC101201077T

Tantoush Z, Mihajlovic L, Kravić B, Ognjenović J, Jankov RM, Ćirković-Veličković T, Stanić-Vučinić D. Digestibility of beta-lactoglobulin following cross-linking by Trametes versicolor laccase and apple polyphenols. in Journal of the Serbian Chemical Society. 2011;76(6):847-855.
doi:10.2298/JSC101201077T .

Tantoush, Ziyad, Mihajlovic, Luka, Kravić, Bojana, Ognjenović, Jana, Jankov, Ratko M., Ćirković-Veličković, Tanja, Stanić-Vučinić, Dragana, "Digestibility of beta-lactoglobulin following cross-linking by Trametes versicolor laccase and apple polyphenols" in Journal of the Serbian Chemical Society, 76, no. 6 (2011):847-855,
https://doi.org/10.2298/JSC101201077T . .